Rabbit Recombinant Monoclonal PRRS virus Nucleocapsid protein antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Transfected cell line - PRRSV, PRRSV samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-PRRS virus Nucleocapsid protein antibody [HL2046] (AB308405), expandable thumbnail](https://content.abcam.com/products/images/prrs-virus-nucleocapsid-protein-antibody-hl2046-ab308405--immunocytochemistry-immunofluorescence-img440694.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PRRS virus Nucleocapsid protein antibody [HL2046] (AB308405)")
![Western blot - Anti-PRRS virus Nucleocapsid protein antibody [HL2046] (AB308405), expandable thumbnail](https://content.abcam.com/products/images/prrs-virus-nucleocapsid-protein-antibody-hl2046-ab308405--western-blot-img441344.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PRRS virus Nucleocapsid protein antibody [HL2046] (AB308405)")
![Western blot - Anti-PRRS virus Nucleocapsid protein antibody [HL2046] (AB308405), expandable thumbnail](https://content.abcam.com/products/images/prrs-virus-nucleocapsid-protein-antibody-hl2046-ab308405--western-blot-img408185.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PRRS virus Nucleocapsid protein antibody [HL2046] (AB308405)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| PRRSV | Tested | Expected |
| Transfected cell line - PRRSV | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - PRRSV | Dilution info 1/500 - 1/3000 | Notes - |
Species PRRSV | Dilution info 1/500 - 1/3000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - PRRSV | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species PRRSV | Dilution info Use at an assay dependent concentration. | Notes - |
Alternative names
Porcine reproductive and respiratory syndrome virus
Rabbit Recombinant Monoclonal PRRS virus Nucleocapsid protein antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Transfected cell line - PRRSV, PRRSV samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Carrier free
- Yes
- Clone number
- HL2046
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-PRRS virus Nucleocapsid protein antibody [HL2046] (ab308405), expandable thumbnail](https://content.abcam.com/products/images/prrs-virus-nucleocapsid-protein-antibody-hl2046-ab308405--immunocytochemistry-immunofluorescence-img440694.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PRRS virus Nucleocapsid protein antibody [HL2046] (ab308405)")
Immunocytochemistry/ Immunofluorescence - Anti-PRRS virus Nucleocapsid protein antibody [HL2046] (ab308405)
Immunofluorescence analysis of 4% paraformaldehyde fixed Mock and transfected 293T cells labelling PRRS virus Nucleocapsid protein using ab308405 at a 1/500 dilution at RT for 15 min (Green). The nuclear counter stain is DAPI (Blue).
![Western blot - Anti-PRRS virus Nucleocapsid protein antibody [HL2046] (ab308405), expandable thumbnail](https://content.abcam.com/products/images/prrs-virus-nucleocapsid-protein-antibody-hl2046-ab308405--western-blot-img441344.jpg "Western blot - Anti-PRRS virus Nucleocapsid protein antibody [HL2046] (ab308405)")
Western blot - Anti-PRRS virus Nucleocapsid protein antibody [HL2046] (ab308405)
Samples were seperated by 15% SDS-PAGE.
All lanes: Western blot - Anti-PRRS virus Nucleocapsid protein antibody [HL2046] (ab308405) at 1/1000 dilution
Lane 1: Non-transfected (–) 293T whole cell extracts at 30 µg
Lane 2: Transfected (+) 293T whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 14 kDa
![Western blot - Anti-PRRS virus Nucleocapsid protein antibody [HL2046] (ab308405), expandable thumbnail](https://content.abcam.com/products/images/prrs-virus-nucleocapsid-protein-antibody-hl2046-ab308405--western-blot-img408185.jpg "Western blot - Anti-PRRS virus Nucleocapsid protein antibody [HL2046] (ab308405)")
Western blot - Anti-PRRS virus Nucleocapsid protein antibody [HL2046] (ab308405)
Non-infected (–) and infected (+) Marc-145 whole cell lysate (5 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PRRS virus Nucleocapsid protein antibody [HL2046] (ab308405) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-PRRS virus Nucleocapsid protein antibody [HL2046] (ab308405) at 1/1000 dilution
Lane 1: Non-infected (–) Marc-145 whole cell lysate at 5 µg
Lanes 2 - 3: Infected (+) Marc-145 whole cell lysate at 5 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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