AB327154

Anti-PRRX1 Antibody [CL14471]

Name: Anti-PRRX1 Antibody [CL14471] (ab327154) | Abcam
Brand: Abcam Limited
SKU: AB327154
Price: 600 USD
Availability: InStock

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Mouse Monoclonal PRX-1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PRRX1.

View Alternative Names

PMX1, PRRX1, Paired mesoderm homeobox protein 1, Homeobox protein PHOX1, Paired-related homeobox protein 1, PRX-1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRRX1 Antibody [CL14471] (AB327154)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRRX1 Antibody [CL14471] (AB327154)

Immunohistochemical staining of human prostate using ab327154 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in glandular cells.

Immunocytochemistry/ Immunofluorescence - Anti-PRRX1 Antibody [CL14471] (AB327154)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PRRX1 Antibody [CL14471] (AB327154)

Immunofluorescence staining in U-138MG cell line using ab327154 at 2–10 µg/ml, showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRRX1 Antibody [CL14471] (AB327154)

Immunohistochemical staining of human endometrium using ab327154 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in glandular cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRRX1 Antibody [CL14471] (AB327154)

Immunohistochemical staining of human uterine cervix using ab327154 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in squamous epithelial cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRRX1 Antibody [CL14471] (AB327154)

Immunohistochemistry analysis in human cervix, uterine and liver tissues using ab327154 at 1 : 50–1 : 200 dilution. Corresponding PRRX1 RNA-seq data are presented for the same tissues.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRRX1 Antibody [CL14471] (AB327154)

Immunohistochemical staining of human liver using ab327154 at 1 : 50–1 : 200 dilution, showing no nuclear positivity in hepatocytes as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL14471

Isotype

IgG3

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human PRRX1.

P54821

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Master transcription factor of stromal fibroblasts for myofibroblastic lineage progression. Orchestrates the functional drift of fibroblasts into myofibroblastic phenotype via TGF-beta signaling by remodeling a super-enhancer landscape. Through this function, plays an essential role in wound healing process (PubMed : 35589735). Acts as a transcriptional regulator of muscle creatine kinase (MCK) and so has a role in the establishment of diverse mesodermal muscle types. The protein binds to an A/T-rich element in the muscle creatine enhancer (By similarity). May play a role in homeostasis and regeneration of bone, white adipose tissue and derm (By similarity).. Isoform 1. Transcriptional activator, when transfected in fibroblastic or myoblastic cell lines. This activity may be masked by the C-terminal OAR domain.. Isoform 2. Transcriptional repressor, when transfected in fibroblastic or myoblastic cell lines.

See full target information PRRX1

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Product promise

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