Rabbit Recombinant Monoclonal PRSS2 antibody. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Western blot - Anti-PRSS2 antibody [EPR25648-5] (AB302603), expandable thumbnail](https://content.abcam.com/products/images/prss2-antibody-epr25648-5-ab302603--western-blot-img401141.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PRSS2 antibody [EPR25648-5] (AB302603)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (AB302603), expandable thumbnail](https://content.abcam.com/products/images/prss2-antibody-epr25648-5-ab302603--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401176.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (AB302603)")
![Western blot - Anti-PRSS2 antibody [EPR25648-5] (AB302603), expandable thumbnail](https://content.abcam.com/products/images/prss2-antibody-epr25648-5-ab302603--western-blot-img401140.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PRSS2 antibody [EPR25648-5] (AB302603)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (AB302603), expandable thumbnail](https://content.abcam.com/products/images/prss2-antibody-epr25648-5-ab302603--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401175.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (AB302603)")
![Western blot - Anti-PRSS2 antibody [EPR25648-5] (AB302603), expandable thumbnail](https://content.abcam.com/products/images/prss2-antibody-epr25648-5-ab302603--western-blot-img401139.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PRSS2 antibody [EPR25648-5] (AB302603)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt (Intra) | IHC-P | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
In the ileum, may be involved in defensin processing, including DEFA5.
Alternative names
TRY2, TRYP2, PRSS2, Trypsin-2, Anionic trypsinogen, Serine protease 2, Trypsin II
Recommended products
Rabbit Recombinant Monoclonal PRSS2 antibody. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR25648-5
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-PRSS2 antibody [EPR25648-5] (ab302603), expandable thumbnail](https://content.abcam.com/products/images/prss2-antibody-epr25648-5-ab302603--western-blot-img401141.jpg "Western blot - Anti-PRSS2 antibody [EPR25648-5] (ab302603)")
Western blot - Anti-PRSS2 antibody [EPR25648-5] (ab302603)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The identity of the band at approximately 80 kDa is unknown.
Exposure time: 3 minutesAll lanes: Western blot - Anti-PRSS2 antibody [EPR25648-5] (ab302603) at 1/1000 dilution
All lanes: HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell fresh lysate 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 30 kDa
Exposure time: 3min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (ab302603), expandable thumbnail](https://content.abcam.com/products/images/prss2-antibody-epr25648-5-ab302603--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401176.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (ab302603)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (ab302603)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PRSS2 with ab302603 at 1/2000 (0.256 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Negative control: no staining on human kidney. The section was incubated with ab302603 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Western blot - Anti-PRSS2 antibody [EPR25648-5] (ab302603), expandable thumbnail](https://content.abcam.com/products/images/prss2-antibody-epr25648-5-ab302603--western-blot-img401140.jpg "Western blot - Anti-PRSS2 antibody [EPR25648-5] (ab302603)")
Western blot - Anti-PRSS2 antibody [EPR25648-5] (ab302603)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with human PRSS1, PRSS3 and PRSS3P2.
Exposure time: 15 secondsAll lanes: Western blot - Anti-PRSS2 antibody [EPR25648-5] (ab302603) at 1/1000 dilution
Lane 1: His-tagged PRSS1 recombinant protein 10 ng
Lane 2: His-tagged PRSS2 recombinant protein 10 ng
Lane 3: His-tagged PRSS3 recombinant protein 10 ng
Lane 4: His-tagged PRSS3P2 recombinant protein 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 25 kDa
Exposure time: 15s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (ab302603), expandable thumbnail](https://content.abcam.com/products/images/prss2-antibody-epr25648-5-ab302603--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401175.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (ab302603)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (ab302603)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PRSS2 with ab302603 at 1/2000 (0.256 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Postitive staining on acinar cells of human pancreas. The section was incubated with ab302603 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Western blot - Anti-PRSS2 antibody [EPR25648-5] (ab302603), expandable thumbnail](https://content.abcam.com/products/images/prss2-antibody-epr25648-5-ab302603--western-blot-img401139.jpg "Western blot - Anti-PRSS2 antibody [EPR25648-5] (ab302603)")
Western blot - Anti-PRSS2 antibody [EPR25648-5] (ab302603)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative controls: kidney, testis (PMID: 9736042)
Exposure time: 3.25 secondsAll lanes: Western blot - Anti-PRSS2 antibody [EPR25648-5] (ab302603) at 1/1000 dilution
Lane 1: Human pancreas tissue lysate 5 µg
Lane 2: Human kidney tissue lysate 5 µg
Lane 3: Human testis tissue lysate 5 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 30 kDa
Exposure time: 3.25s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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