Anti-PRSS2 antibody [EPR25648-5]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (AB302603)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PRSS2 with ab302603 at 1/2000 (0.256 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining on human kidney. The section was incubated with ab302603 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (AB302603)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PRSS2 with ab302603 at 1/2000 (0.256 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Postitive staining on acinar cells of human pancreas. The section was incubated with ab302603 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-PRSS2 antibody [EPR25648-5] (AB302603)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative controls : kidney, testis (PMID : 9736042) Exposure time : 3.25 seconds

All lanes:

Western blot - Anti-PRSS2 antibody [EPR25648-5] (ab302603) at 1/1000 dilution

Lane 1:

Human pancreas tissue lysate 5 µg

Lane 2:

Human kidney tissue lysate 5 µg

Lane 3:

Human testis tissue lysate 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa

false

Exposure time: 3.25s

• WB

Supplier Data

Western blot - Anti-PRSS2 antibody [EPR25648-5] (AB302603)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The identity of the band at approximately 80 kDa is unknown. Exposure time : 3 minutes

All lanes:

Western blot - Anti-PRSS2 antibody [EPR25648-5] (ab302603) at 1/1000 dilution

All lanes:

HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell fresh lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-PRSS2 antibody [EPR25648-5] (AB302603)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This antibody does not cross-react with human PRSS1, PRSS3 and PRSS3P2.

All lanes:

Western blot - Anti-PRSS2 antibody [EPR25648-5] (ab302603) at 1/1000 dilution

Lane 1:

His-tagged PRSS1 recombinant protein 10 ng

Lane 2:

PRSS2 recombinant protein without a tag at 10 ng

Lane 3:

His-tagged PRSS3 recombinant protein at 10 ng

Lane 4:

His-tagged PRSS3P2 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 25 kDa

false

Exposure time: 15s