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AB302604

Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free)

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Rabbit Recombinant Monoclonal PRSS2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

TRY2, TRYP2, PRSS2, Trypsin-2, Anionic trypsinogen, Serine protease 2, Trypsin II

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)

This data was developed using ab302603, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PRSS2 with ab302603 at 1/2000 (0.256 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining on human kidney. The section was incubated with ab302603 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)

This data was developed using ab302603, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PRSS2 with ab302603 at 1/2000 (0.256 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Postitive staining on acinar cells of human pancreas. The section was incubated with ab302603 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Western blot - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)
  • WB

Supplier Data

Western blot - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)

This data was developed using ab302603, the same antibody clone in a different buffer Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative controls : kidney, testis (PMID : 9736042) Exposure time : 3.25 seconds

All lanes:

Western blot - Anti-PRSS2 antibody [EPR25648-5] (<a href='/en-us/products/primary-antibodies/prss2-antibody-epr25648-5-ab302603'>ab302603</a>) at 1/1000 dilution

Lane 1:

Human pancreas tissue lysate 5 µg

Lane 2:

Human kidney tissue lysate 5 µg

Lane 3:

Human testis tissue lysate 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa

false

Exposure time: 3.25s

Western blot - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)
  • WB

Supplier Data

Western blot - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)

This data was developed using ab302603, the same antibody clone in a different buffer Blocking and diluting buffer and concentration : 5% NFDM/TBST The identity of the band at approximately 80 kDa is unknown. Exposure time : 3 minutes

All lanes:

Western blot - Anti-PRSS2 antibody [EPR25648-5] (<a href='/en-us/products/primary-antibodies/prss2-antibody-epr25648-5-ab302603'>ab302603</a>) at 1/1000 dilution

All lanes:

HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell fresh lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa

false

Exposure time: 3min

Western blot - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)
  • WB

Supplier Data

Western blot - Anti-PRSS2 antibody [EPR25648-5] (BSA and Azide free) (AB302604)

This data was developed using ab302603, the same antibody clone in a different buffer.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human PRSS1, PRSS3 and PRSS3P2.

All lanes:

Western blot - Anti-PRSS2 antibody [EPR25648-5] (<a href='/en-us/products/primary-antibodies/prss2-antibody-epr25648-5-ab302603'>ab302603</a>) at 1/1000 dilution

Lane 1:

His-tagged PRSS1 recombinant protein 10 ng

Lane 2:

PRSS2 recombinant protein without a tag at 10 ng

Lane 3:

His-tagged PRSS3 recombinant protein at 10 ng

Lane 4:

His-tagged PRSS3P2 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 25 kDa

false

Exposure time: 15s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25648-5

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Primary Antibodies

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Assay Kits

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Primary Antibodies

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Reactivity data

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

In the ileum, may be involved in defensin processing, including DEFA5.
See full target information PRSS2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com