Rabbit Recombinant Monoclonal PRSS2 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Recombinant full length protein - Human samples.
Images
![Western blot - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (AB302605), expandable thumbnail](https://content.abcam.com/products/images/prss2prss3-antibody-epr25648-13-ab302605--western-blot-img401137.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (AB302605)")
![Western blot - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (AB302605), expandable thumbnail](https://content.abcam.com/products/images/prss2prss3-antibody-epr25648-13-ab302605--western-blot-img401138.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (AB302605)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (AB302605), expandable thumbnail](https://content.abcam.com/products/images/prss2prss3-antibody-epr25648-13-ab302605--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401174.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (AB302605)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (AB302605), expandable thumbnail](https://content.abcam.com/products/images/prss2prss3-antibody-epr25648-13-ab302605--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401173.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (AB302605)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Recombinant full length protein - Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
Target data
Function
In the ileum, may be involved in defensin processing, including DEFA5.
Additional Targets
PRSS3
Alternative names
TRY2, TRYP2, PRSS2, Trypsin-2, Anionic trypsinogen, Serine protease 2, Trypsin II
Recommended products
Rabbit Recombinant Monoclonal PRSS2 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Recombinant full length protein - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR25648-13
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (ab302605), expandable thumbnail](https://content.abcam.com/products/images/prss2prss3-antibody-epr25648-13-ab302605--western-blot-img401137.jpg "Western blot - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (ab302605)")
Western blot - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (ab302605)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
Negative control: kidney, testis (PMID: 9736042).
All lanes: Western blot - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (ab302605) at 1/1000 dilution
Lane 1: Human pancreas tissue lysate at 5 µg
Lane 2: Human kidney tissue lysate at 5 µg
Lane 3: Human testis tissue lysate at 5 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 30 kDa
Exposure time: 3min
![Western blot - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (ab302605), expandable thumbnail](https://content.abcam.com/products/images/prss2prss3-antibody-epr25648-13-ab302605--western-blot-img401138.jpg "Western blot - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (ab302605)")
Western blot - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (ab302605)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody reacts with human PRSS2 and PRSS3 but not PRSS1 PRSS3P2.
Exposure time: 15 secondsAll lanes: Western blot - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (ab302605) at 1/1000 dilution
Lane 1: His-tagged PRSS1 recombinant protein 10 ng
Lane 2: His-tagged PRSS2 recombinant protein 10 ng
Lane 3: His-tagged PRSS3 recombinant protein 10 ng
Lane 4: His-tagged PRSS3P2 recombinant protein 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 30 kDa
Exposure time: 15s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (ab302605), expandable thumbnail](https://content.abcam.com/products/images/prss2prss3-antibody-epr25648-13-ab302605--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401174.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (ab302605)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (ab302605)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PRSS2+PRSS3 with ab302605 at 1/2000 (0.257 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Negative control: no staining on human kidney. The section was incubated with ab302605 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (ab302605), expandable thumbnail](https://content.abcam.com/products/images/prss2prss3-antibody-epr25648-13-ab302605--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img401173.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (ab302605)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (ab302605)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PRSS2+PRSS3 with ab302605 at 1/2000 (0.257 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on human pancreas. The section was incubated with ab302605 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Downloads
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- Visit the general protocols
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