Anti-PRSS2+PRSS3 antibody [EPR25648-13] (BSA and Azide free)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (BSA and Azide free) (AB302606)

This data was developed using ab302605, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PRSS2+PRSS3 with ab302605 at 1/2000 (0.257 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining on human kidney. The section was incubated with ab302605 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (BSA and Azide free) (AB302606)

This data was developed using ab302605, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PRSS2+PRSS3 with ab302605 at 1/2000 (0.257 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human pancreas. The section was incubated with ab302605 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (BSA and Azide free) (AB302606)

This data was developed using ab302605, the same antibody clone in a different buffer Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody reacts with human PRSS2 and PRSS3 but not PRSS1 PRSS3P2. Exposure time : 15 seconds

All lanes:

Western blot - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (<a href='/en-us/products/primary-antibodies/prss2prss3-antibody-epr25648-13-ab302605'>ab302605</a>) at 1/1000 dilution

Lane 1:

His-tagged PRSS1 recombinant protein 10 ng

Lane 2:

His-tagged PRSS2 recombinant protein 10 ng

Lane 3:

His-tagged PRSS3 recombinant protein 10 ng

Lane 4:

His-tagged PRSS3P2 recombinant protein 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (BSA and Azide free) (AB302606)

This data was developed using ab302605, the same antibody clone in a different buffer

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

Negative control : kidney, testis (PMID : 9736042)

All lanes:

Western blot - Anti-PRSS2+PRSS3 antibody [EPR25648-13] (<a href='/en-us/products/primary-antibodies/prss2prss3-antibody-epr25648-13-ab302605'>ab302605</a>) at 1/1000 dilution

Lane 1:

Human pancreas tissue lysate at 5 µg

Lane 2:

Human kidney tissue lysate at 5 µg

Lane 3:

Human testis tissue lysate at 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30 kDa

true

Exposure time: 3min