Mouse Polyclonal PRUNE antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRUNE1.
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Publications
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- Cells 12:2023Generation of Conditional Knockout Alleles for PRUNE-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoli Wu,Louise R Simard,Hao DingPubMed 36831191
- Veterinary dermatology 30:446-4472019Evaluation of the expression and distribution of Prune-1 in the skin of healthy dogs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDomenico Santoro,Linda Archer,Antonio Di LoriaPubMed 31286578
- Brain : a journal of neurology 140:940-9522017PRUNE is crucial for normal brain development and mutated in microcephaly with neurodevelopmental impairment.Applications:IFReactive species:Unspecified reactive speciesMassimo Zollo,Mustafa Ahmed,Veronica Ferrucci,Vincenzo Salpietro,Fatemeh Asadzadeh,Marianeve Carotenuto,Reza Maroofian,Ahmed Al-Amri,Royana Singh,Iolanda Scognamiglio,Majid Mojarrad,Luca Musella,Angela Duilio,Angela Di Somma,Ender Karaca,Anna Rajab,Aisha Al-Khayat,Tribhuvan Mohan Mohapatra,Atieh Eslahi,Farah Ashrafzadeh,Lettie E Rawlins,Rajniti Prasad,Rashmi Gupta,Preeti Kumari,Mona Srivastava,Flora Cozzolino,Sunil Kumar Rai,Maria Monti,Gaurav V Harlalka,Michael A Simpson,Philip Rich,Fatema Al-Salmi,Michael A Patton,Barry A Chioza,Stephanie Efthymiou,Francesca Granata,Gabriella Di Rosa,Sarah Wiethoff,Eugenia Borgione,Carmela Scuderi,Kshitij Mankad,Michael G Hanna,Piero Pucci,Henry Houlden,James R Lupski,Andrew H Crosby,Emma L BaplePubMed 28334956
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human PRUNE1. Database link Q86TP1
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, migration and differentiation, and acts as a negative regulator of NME1. Plays a role in the regulation of neurogenesis (PubMed:28334956). Involved in the regulation of microtubule polymerization (PubMed:28334956).
Alternative names
PRUNE, PRUNE1, Exopolyphosphatase PRUNE1, Drosophila-related expressed sequence 17, HTcD37, Protein prune homolog 1, DRES-17, DRES17, hPrune
Recommended products
Mouse Polyclonal PRUNE antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRUNE1.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human PRUNE1. Database link Q86TP1
- Carrier free
- Yes
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-PRUNE antibody (ab88613)
All lanes: Western blot - Anti-PRUNE antibody (ab88613) at 1 µg/mL
All lanes: Raw 264.7 cell lysate at 50 µg
Predicted band size: 50 kDa
Observed band size: 60 kDa
Western blot - Anti-PRUNE antibody (ab88613)
All lanes: Western blot - Anti-PRUNE antibody (ab88613) at 1 µg/mL
All lanes: NIH/3T3 cell lysate at 50 µg
Predicted band size: 50 kDa
Observed band size: 60 kDa
Western blot - Anti-PRUNE antibody (ab88613)
All lanes: Western blot - Anti-PRUNE antibody (ab88613) at 1 µg/mL
Lane 1: PRUNE transfected 293T cell lysate at 25 µg
Lane 2: Non transfected 293T cell lysate at 25 µg
Predicted band size: 50 kDa
Observed band size: 60 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PRUNE antibody (ab88613)
ab88613, at 10 μg/ml, staining PRUNE in HeLa cell by Immunofluorescence.
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