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AB88613

Anti-PRUNE antibody

4

(2 Reviews)

|

(4 Publications)

Mouse Polyclonal PRUNE antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRUNE1.

View Alternative Names

PRUNE, PRUNE1, Exopolyphosphatase PRUNE1, Drosophila-related expressed sequence 17, HTcD37, Protein prune homolog 1, DRES-17, DRES17, hPrune

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-PRUNE antibody (AB88613)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PRUNE antibody (AB88613)

ab88613, at 10 μg/ml, staining PRUNE in HeLa cell by Immunofluorescence.

Western blot - Anti-PRUNE antibody (AB88613)
  • WB

Unknown

Western blot - Anti-PRUNE antibody (AB88613)

All lanes:

Western blot - Anti-PRUNE antibody (ab88613) at 1 µg/mL

All lanes:

Raw 264.7 cell lysate at 50 µg

Predicted band size: 50 kDa

Observed band size: 60 kDa

false

Western blot - Anti-PRUNE antibody (AB88613)
  • WB

Unknown

Western blot - Anti-PRUNE antibody (AB88613)

All lanes:

Western blot - Anti-PRUNE antibody (ab88613) at 1 µg/mL

Lane 1:

PRUNE transfected 293T cell lysate at 25 µg

Lane 2:

Non transfected 293T cell lysate at 25 µg

Predicted band size: 50 kDa

Observed band size: 60 kDa

false

Western blot - Anti-PRUNE antibody (AB88613)
  • WB

Unknown

Western blot - Anti-PRUNE antibody (AB88613)

All lanes:

Western blot - Anti-PRUNE antibody (ab88613) at 1 µg/mL

All lanes:

NIH/3T3 cell lysate at 50 µg

Predicted band size: 50 kDa

Observed band size: 60 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PRUNE1.

Q86TP1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, migration and differentiation, and acts as a negative regulator of NME1. Plays a role in the regulation of neurogenesis (PubMed : 28334956). Involved in the regulation of microtubule polymerization (PubMed : 28334956).
See full target information PRUNE1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38612726

2024

Targeting Group 3 Medulloblastoma by the Anti-PRUNE-1 and Anti-LSD1/KDM1A Epigenetic Molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Bibbò,Fatemeh Asadzadeh,Angelo Boccia,Carmen Sorice,Orazio Bianco,Carmen Daniela Saccà,Barbara Majello,Vittoria Donofrio,Delfina Bifano,Lucia De Martino,Lucia Quaglietta,Adriana Cristofano,Eugenio Maria Covelli,Giuseppe Cinalli,Veronica Ferrucci,Pasqualino De Antonellis,Massimo Zollo

Cells 12: PubMed36831191

2023

Generation of Conditional Knockout Alleles for PRUNE-1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Wu,Louise R Simard,Hao Ding

Veterinary dermatology 30:446-447 PubMed31286578

2019

Evaluation of the expression and distribution of Prune-1 in the skin of healthy dogs.

Applications

Unspecified application

Species

Unspecified reactive species

Domenico Santoro,Linda Archer,Antonio Di Loria

Brain : a journal of neurology 140:940-952 PubMed28334956

2017

PRUNE is crucial for normal brain development and mutated in microcephaly with neurodevelopmental impairment.

Applications

IF

Species

Unspecified reactive species

Massimo Zollo,Mustafa Ahmed,Veronica Ferrucci,Vincenzo Salpietro,Fatemeh Asadzadeh,Marianeve Carotenuto,Reza Maroofian,Ahmed Al-Amri,Royana Singh,Iolanda Scognamiglio,Majid Mojarrad,Luca Musella,Angela Duilio,Angela Di Somma,Ender Karaca,Anna Rajab,Aisha Al-Khayat,Tribhuvan Mohan Mohapatra,Atieh Eslahi,Farah Ashrafzadeh,Lettie E Rawlins,Rajniti Prasad,Rashmi Gupta,Preeti Kumari,Mona Srivastava,Flora Cozzolino,Sunil Kumar Rai,Maria Monti,Gaurav V Harlalka,Michael A Simpson,Philip Rich,Fatema Al-Salmi,Michael A Patton,Barry A Chioza,Stephanie Efthymiou,Francesca Granata,Gabriella Di Rosa,Sarah Wiethoff,Eugenia Borgione,Carmela Scuderi,Kshitij Mankad,Michael G Hanna,Piero Pucci,Henry Houlden,James R Lupski,Andrew H Crosby,Emma L Baple
View all publications
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Product promise

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