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Rabbit Recombinant Monoclonal PSAP antibody. Suitable for WB, IHC-P, ICC/IF, IP and reacts with Human samples.

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Images

Western blot - Anti-PSAP antibody [EPR25649-20] (AB308122), expandable thumbnail
  • Western blot - Anti-PSAP antibody [EPR25649-20] (AB308122), expandable thumbnail
  • Western blot - Anti-PSAP antibody [EPR25649-20] (AB308122), expandable thumbnail
  • Immunoprecipitation - Anti-PSAP antibody [EPR25649-20] (AB308122), expandable thumbnail
  • Immunohistochemistry - Anti-PSAP antibody [EPR25649-20] (AB308122), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFIPFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases.Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12).ProsaposinBehaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling.Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal PSAP antibody. Suitable for WB, IHC-P, ICC/IF, IP and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR25649-20

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-PSAP antibody [EPR25649-20] (ab308122), expandable thumbnail

    Western blot - Anti-PSAP antibody [EPR25649-20] (ab308122)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    Lysates were freshly made and used immediately to minimize protein degradation.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15743835) (PMID: 15353235).

    Exposure time:

    Lane 1: 180 seconds

    Lane 2: 15 seconds

    All lanes: Western blot - Anti-PSAP antibody [EPR25649-20] (ab308122) at 1/1000 dilution

    Lane 1: HeLa whole cell lysate at 20 µg

    Lane 2: PC-3 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 70 kDa

  • Western blot - Anti-PSAP antibody [EPR25649-20] (ab308122), expandable thumbnail

    Western blot - Anti-PSAP antibody [EPR25649-20] (ab308122)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    The band below 70 kDa may be caused by degradation.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PSAP antibody [EPR25649-20] (ab308122) at 1/1000 dilution

    All lanes: Human cerebellum tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 70 kDa

    Exposure time: 180s

  • Western blot - Anti-PSAP antibody [EPR25649-20] (ab308122), expandable thumbnail

    Western blot - Anti-PSAP antibody [EPR25649-20] (ab308122)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15743835) (PMID: 15353235).

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PSAP antibody [EPR25649-20] (ab308122) at 1/1000 dilution

    Lane 1: 293T transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: 293T transfected with siRNA specifically targeting PSAP whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 70 kDa

    Exposure time: 103s

  • Immunoprecipitation - Anti-PSAP antibody [EPR25649-20] (ab308122), expandable thumbnail

    Immunoprecipitation - Anti-PSAP antibody [EPR25649-20] (ab308122)

    PSAP was immunoprecipitated from 0.35 mg PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate with ab308122 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308122 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: PC-3 whole cell lysate

    Lane 2: ab308122 IP in PC-3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab308122 in PC-3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds.

    All lanes: Immunoprecipitation - Anti-PSAP antibody [EPR25649-20] (ab308122) at 1/30 dilution

    Lane 1: PC-3 whole cell lysate at 20 µg

    Lane 2: PC-3 whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 70 kDa

    Exposure time: 8s

  • Immunohistochemistry - Anti-PSAP antibody [EPR25649-20] (ab308122), expandable thumbnail

    Immunohistochemistry - Anti-PSAP antibody [EPR25649-20] (ab308122)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling PSAP with ab308122 at 1/2000 followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection). Positive cytoplasmic staining on human spleen. The section was incubated with ab308122 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-PSAP antibody [EPR25649-20] (ab308122), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PSAP antibody [EPR25649-20] (ab308122)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labelling PSAP with ab308122 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).
    Confocal image showing cytoplasmic staining in PC-3 cell line.
    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Immunohistochemistry - Anti-PSAP antibody [EPR25649-20] (ab308122), expandable thumbnail

    Immunohistochemistry - Anti-PSAP antibody [EPR25649-20] (ab308122)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling PSAP with ab308122 at 1/2000 followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection). Positive cytoplasmic staining on human cerebrum. The section was incubated with ab308122 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-PSAP antibody [EPR25649-20] (ab308122), expandable thumbnail

    Western blot - Anti-PSAP antibody [EPR25649-20] (ab308122)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15743835) (PMID: 15353235).

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    Exposure time: 180 seconds.

    All lanes: Western blot - Anti-PSAP antibody [EPR25649-20] (ab308122) at 1/1000 dilution

    Lane 1: HeLa transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HeLa transfected with siRNA specifically targeting PSAP whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 70 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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