Anti-PSAP antibody [EPR25649-20] - BSA and Azide free

8 product images

• 

  Western blot - Anti-PSAP antibody [EPR25649-20] - BSA and Azide free (ab308123)

  This data was developed using Anti-PSAP antibody [EPR25649-20] ab308122, the same antibody clone in a different buffer formulation.
  

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

  Lysates were freshly made and used immediately to minimize protein degradation.

  The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15743835) (PMID: 15353235).

  Exposure time:

  Lane 1: 180 seconds

  Lane 2: 15 seconds

  All lanes: Western blot - Anti-PSAP antibody [EPR25649-20] (Anti-PSAP antibody [EPR25649-20] ab308122) at 1/1000 dilution

  Lane 1: HeLa whole cell lysate at 20 µg

  Lane 2: PC-3 whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Developed using the ECL technique.

  Observed band size: 70 kDa

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  

  This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

  Lysates were freshly made and used immediately to minimize protein degradation.

  The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15743835) (PMID: 15353235).

  Exposure time:

  Lane 1: 180 seconds

  Lane 2: 15 seconds

• 

  Western blot - Anti-PSAP antibody [EPR25649-20] - BSA and Azide free (ab308123)

  This data was developed using Anti-PSAP antibody [EPR25649-20] ab308122, the same antibody clone in a different buffer formulation.
  

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

  The band below 70 kDa may be caused by degradation.

  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

  All lanes: Western blot - Anti-PSAP antibody [EPR25649-20] (Anti-PSAP antibody [EPR25649-20] ab308122) at 1/1000 dilution

  All lanes: Human cerebellum tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Developed using the ECL technique.

  Observed band size: 70 kDa

  Exposure time: 180s

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  

  This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

  The band below 70 kDa may be caused by degradation.

  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

• 

  Western blot - Anti-PSAP antibody [EPR25649-20] - BSA and Azide free (ab308123)

  This data was developed using Anti-PSAP antibody [EPR25649-20] ab308122, the same antibody clone in a different buffer formulation.
  

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15743835) (PMID: 15353235).

  This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

  All lanes: Western blot - Anti-PSAP antibody [EPR25649-20] (Anti-PSAP antibody [EPR25649-20] ab308122) at 1/1000 dilution

  Lane 1: 293T transfected with scrambled siRNA control whole cell lysate at 20 µg

  Lane 2: 293T transfected with siRNA specifically targeting PSAP whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Developed using the ECL technique.

  Observed band size: 70 kDa

  Exposure time: 103s

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  

  The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15743835) (PMID: 15353235).

  This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

• 

  Immunoprecipitation - Anti-PSAP antibody [EPR25649-20] - BSA and Azide free (ab308123)

  This data was developed using Anti-PSAP antibody [EPR25649-20] ab308122, the same antibody clone in a different buffer formulation.
  
  PSAP was immunoprecipitated from 0.35 mg PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate with Anti-PSAP antibody [EPR25649-20] ab308122 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-PSAP antibody [EPR25649-20] ab308122 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
  
  Lane 1: PC-3 whole cell lysate
  
  Lane 2: Anti-PSAP antibody [EPR25649-20] ab308122 IP in PC-3 whole cell lysate
  
  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PSAP antibody [EPR25649-20] ab308122 in PC-3 whole cell lysate
  
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  
  Exposure time: 8 seconds.

  All lanes: Immunoprecipitation - Anti-PSAP antibody [EPR25649-20] (Anti-PSAP antibody [EPR25649-20] ab308122) at 1/30 dilution

  Lane 1: PC-3 whole cell lysate at 20 µg

  Lane 2: PC-3 whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Observed band size: 70 kDa

  Exposure time: 8s

  PSAP was immunoprecipitated from 0.35 mg PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate with Anti-PSAP antibody [EPR25649-20] ab308122 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-PSAP antibody [EPR25649-20] ab308122 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
  
  Lane 1: PC-3 whole cell lysate
  
  Lane 2: Anti-PSAP antibody [EPR25649-20] ab308122 IP in PC-3 whole cell lysate
  
  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PSAP antibody [EPR25649-20] ab308122 in PC-3 whole cell lysate
  
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  
  Exposure time: 8 seconds.

• 

  Immunohistochemistry - Anti-PSAP antibody [EPR25649-20] - BSA and Azide free (ab308123)

  This data was produced using Anti-PSAP antibody [EPR25649-20] ab308122, the same antibody clone but in a different formulation.
  
  Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling PSAP with Anti-PSAP antibody [EPR25649-20] ab308122 at 1/2000 followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection). Positive cytoplasmic staining on human spleen. The section was incubated with Anti-PSAP antibody [EPR25649-20] ab308122 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-PSAP antibody [EPR25649-20] - BSA and Azide free (ab308123)

  This data was produced using Anti-PSAP antibody [EPR25649-20] ab308122, the same antibody clone but in a different formulation.
  
  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labelling PSAP with Anti-PSAP antibody [EPR25649-20] ab308122 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).
  Confocal image showing cytoplasmic staining in PC-3 cell line.
  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).
  
  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• 

  Immunohistochemistry - Anti-PSAP antibody [EPR25649-20] - BSA and Azide free (ab308123)

  This data was produced using Anti-PSAP antibody [EPR25649-20] ab308122, the same antibody clone but in a different formulation.
  
  Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling PSAP with Anti-PSAP antibody [EPR25649-20] ab308122 at 1/2000 followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection). Positive cytoplasmic staining on human cerebrum. The section was incubated with Anti-PSAP antibody [EPR25649-20] ab308122 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Western blot - Anti-PSAP antibody [EPR25649-20] - BSA and Azide free (ab308123)

  This data was developed using Anti-PSAP antibody [EPR25649-20] ab308122, the same antibody clone in a different buffer formulation.
  
  Blocking and diluting buffer and concentration: The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15743835) (PMID: 15353235).
  
  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
  
  Exposure time: 180 seconds

  All lanes: Western blot - Anti-PSAP antibody [EPR25649-20] (Anti-PSAP antibody [EPR25649-20] ab308122) at 1/1000 dilution

  Lane 1: HeLa transfected with scrambled siRNA control whole cell lysate at 20 µg

  Lane 2: HeLa transfected with siRNA specifically targeting PSAP whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 70 kDa

  Exposure time: 180s

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  
  The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15743835) (PMID: 15353235).
  
  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
  
  Exposure time: 180 seconds.