Rabbit Recombinant Monoclonal PSD93 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse, Human, Rat, Recombinant fragment - Human samples.
pH: 7.2 - 7.4
Constituents: 100% PBS
ICC/IF | IP | WB | Flow Cyt (Intra) | IHC-P | |
---|---|---|---|---|---|
Human | Not recommended | Tested | Tested | Not recommended | Tested |
Mouse | Not recommended | Tested | Tested | Not recommended | Tested |
Rat | Not recommended | Expected | Tested | Not recommended | Tested |
Recombinant fragment - Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human, Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses (By similarity).
Disks large homolog 2, Channel-associated protein of synapse-110, Postsynaptic density protein PSD-93, Chapsyn-110, DLG2
Rabbit Recombinant Monoclonal PSD93 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse, Human, Rat, Recombinant fragment - Human samples.
pH: 7.2 - 7.4
Constituents: 100% PBS
DLG2 is an alternative name for PSD93.
ab289997 is the carrier-free version of Anti-PSD93 antibody [EPR26468-112] ab289972.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-PSD93 antibody [EPR26468-112] ab289972, the same antibody clone in a different buffer formulation.
PSD93 was immunoprecipitated from Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with Anti-PSD93 antibody [EPR26468-112] ab289972 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-PSD93 antibody [EPR26468-112] ab289972 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug
Lane 2: Anti-PSD93 antibody [EPR26468-112] ab289972 IP in Neuro-2a whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PSD93 antibody [EPR26468-112] ab289972 in Neuro-2a whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
All lanes: Immunoprecipitation - Anti-PSD93 antibody [EPR26468-112] (Anti-PSD93 antibody [EPR26468-112] ab289972)
Predicted band size: 97 kDa
Observed band size: 25-110 kDa
This data was developed using Anti-PSD93 antibody [EPR26468-112] ab289972, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling PSD93 with Anti-PSD93 antibody [EPR26468-112] ab289972 at 1/500 followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on human cerebrum. The section was incubated with Anti-PSD93 antibody [EPR26468-112] ab289972 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS instead of primary antibody, followed by LeicaDS9800 (Bond™, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using Anti-PSD93 antibody [EPR26468-112] ab289972, the same antibody clone in a different buffer formulation.
PSD93 was immunoprecipitated from SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with Anti-PSD93 antibody [EPR26468-112] ab289972 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-PSD93 antibody [EPR26468-112] ab289972 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 10 ug
Lane 2: Anti-PSD93 antibody [EPR26468-112] ab289972 IP in SH-SY5Y whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PSD93 antibody [EPR26468-112] ab289972 in SH-SY5Y whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
All lanes: Immunoprecipitation - Anti-PSD93 antibody [EPR26468-112] (Anti-PSD93 antibody [EPR26468-112] ab289972)
Predicted band size: 97 kDa
Observed band size: 25-110 kDa
This data was developed using Anti-PSD93 antibody [EPR26468-112] ab289972, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
DLG2 has many splicing forms. The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 14724236).
Negative control: spleen (PMID: 7590743)
Exposure time: 15 seconds
All lanes: Western blot - Anti-PSD93 antibody [EPR26468-112] (Anti-PSD93 antibody [EPR26468-112] ab289972) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse spleen tissue lysate at 20 µg
Lane 3: Rat brain tissue lysate at 20 µg
Lane 4: Rat spleen tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 97 kDa
Observed band size: 25-110 kDa
This data was developed using Anti-PSD93 antibody [EPR26468-112] ab289972, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling PSD93 with Anti-PSD93 antibody [EPR26468-112] ab289972 at 1/500 followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with Anti-PSD93 antibody [EPR26468-112] ab289972 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: PBS instead of primary antibody, followed by LeicaDS9800 (Bond™, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using Anti-PSD93 antibody [EPR26468-112] ab289972, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling PSD93 with Anti-PSD93 antibody [EPR26468-112] ab289972 at 1/500 followed by LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on mouse cerebrum. The section was incubated with Anti-PSD93 antibody [EPR26468-112] ab289972 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using Anti-PSD93 antibody [EPR26468-112] ab289972, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with human DLG1.
Both recombinant proteins were made in-house and expressed from E.coli expression systems.
All lanes: Western blot - Anti-PSD93 antibody [EPR26468-112] (Anti-PSD93 antibody [EPR26468-112] ab289972) at 1/1000 dilution
Lane 1: His-tagged Human DLG2 recombinant protein (20ng)
Lane 2: His-tagged Human DLG1 recombinant protein (20ng)
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 97 kDa
Observed band size: 25-110 kDa
This data was developed using Anti-PSD93 antibody [EPR26468-112] ab289972, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling PSD93 with Anti-PSD93 antibody [EPR26468-112] ab289972 at 1/500 (1.222 followed by LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Negative control: No staining on human cardiac muscle. The section was incubated with Anti-PSD93 antibody [EPR26468-112] ab289972 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin..
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
This data was developed using Anti-PSD93 antibody [EPR26468-112] ab289972, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
DLG2 has many splicing forms. The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 14724236).
Exposure time: 5.5 seconds
All lanes: Western blot - Anti-PSD93 antibody [EPR26468-112] (Anti-PSD93 antibody [EPR26468-112] ab289972) at 1/1000 dilution
All lanes: Human cerebellum tissue lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 97 kDa
Observed band size: 25-110 kDa
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