Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker

• WB

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Western blot - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (AB13552)

Western blot analysis of bovine brain tissue extract, probed with ab13552. Western blot analysis of bovine brain tissue extract, probed with ab13552.

All lanes:

Western blot - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552)

Predicted band size: 80 kDa

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• Flow Cyt

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Flow Cytometry - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (AB13552)

Overlay histogram showing K562 cells stained with ab13552 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13552, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (AB13552)

Immunocytochemistry/ Immunofluorescence analysis of cells labeling PSD95 with ab13552 at 1/100 dilution. Cells were fixed with 00% cold methanol for 10 minutes at -20°C. A FITC Goat Anti-Mouse antibody at 1/50 was used as secondary. The image shows Filamentouslike staining.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (AB13552)

Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue* labelling PSD95 with ab13552 at 1ug/ml followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Positive staining in human cerebral cortex.

The section was incubated with ab13552 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0 epitope retrieval solution1) for 20 mins.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

• ICC/IF

AbReview17765****

Immunocytochemistry/ Immunofluorescence - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (AB13552)

Central Panel : ab13552 staining PSD95 in rat primary hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS-BSA 3%) for 30 minutes at 20°C. An Cy5^®-conjugated Donkey polyclonal to mouse IgG (dilution 1/500) was used as secondary antibody.
Left-hand panel : Homer GFP.
Right-hand panel : Merge.

This image is courtesy of an anonymous Abreview

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (AB13552)

ab13552 at 1/100 dilution staining PSD95 in mouse backskin tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer. The image show basal, hair follicle and dermal staining.

• WB

Supplier Data

Western blot - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (AB13552)

All lanes:

Western blot - Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552) at 1/1000 dilution

All lanes:

Rat brain membrane lysate.

Predicted band size: 80 kDa

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