Name: Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker
SKU: ab238135
Rating: 5 (4 reviews)

Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker is a rabbit recombinant monoclonal antibody that is used to detect PSD95 in IHC-Fr, IHC-P, IP, Western blot. Suitable for Human, Mouse, Rat samples.

- Specificity confirmed with Dlg4 knockout cell line validation
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Cited in over 20 publications

Related conjugates and formulations

ab269863
Carrier free
Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free
ab302806
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Alexa Fluor® 568 Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker
ab302566
Conjugated
Alexa Fluor® 647 Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker
ab302571
Conjugated
Alexa Fluor® 555 Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker
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Carrier free
Anti-PSD95 antibody [EPR23124-118] – Goat IgG (Chimeric) – BSA and Azide Free

Images

Western blot - Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (AB238135), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	IHC-P	ICC/IF	IHC-Fr
Human	Expected	Tested	Expected	Not recommended	Expected
Mouse	Tested	Tested	Tested	Not recommended	Tested
Rat	Expected	Tested	Tested	Not recommended	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/2000	Notes -
Species Rat	Dilution info 1/2000	Notes -
Species Human	Dilution info 1/2000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -
Species Rat	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -
Species Rat	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information Dlg4

Function

Postsynaptic scaffolding protein that plays a critical role in synaptogenesis and synaptic plasticity by providing a platform for the postsynaptic clustering of crucial synaptic proteins (PubMed:15358775, PubMed:9853749). Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression (Probable). Under basal conditions, cooperates with FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane through FYN-mediated phosphorylation of ZDHHC5 and its subsequent inhibition of association with endocytic proteins (By similarity).

Alternative names

Dlgh4, Psd95, Dlg4, Disks large homolog 4, Postsynaptic density protein 95, Synapse-associated protein 90, PSD-95, SAP-90, SAP90

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR23124-118
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (ab238135) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-Fr, IHC-P, IP, WB in human, mouse, rat samples.

Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (ab238135) has been cited over 24 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality manufacturing and validation processes ensure Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (ab238135) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

The specificity of Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (ab238135) has been confirmed by testing in knockout samples.

Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (ab238135) has 4 independent reviews from customers.

Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (ab238135) specifically detects PSD95 (UniProt ID: Q62108; Molecular weight: 80kDa) and is sold in 100 uL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EPR23124-118 - Anti-PSD95 antibody [EPR23124-118] - BSA and Azide free ab269863.

Antibody clone EPR23124-118 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 568 (Anti-DNA PKcs antibody [Y393] ab32566, Anti-PYK2 antibody [YE353] ab32571, ab3286).

Post Synaptic Density 95 kDa (PSD-95), also known as synapse-associated protein 90 kDa (SAP90), is a vital protein located in the postsynaptic density of neurons. It plays a key role in synaptic signaling and plasticity by organizing and anchoring various synaptic proteins, such as NMDA receptors and potassium channels. Changes in PSD-95 expression are associated with several neurological and psychiatric disorders, including schizophrenia, autism, and Alzheimer's disease (AD). Research on PSD-95 is crucial for understanding synaptic function and dysfunction, emphasizing its importance in the study of neurodegenerative and neuropsychiatric diseases.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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9 product images

Western blot - Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (ab238135)
Lanes 1 - 4: Merged signal (red and green). Green - ab238135 observed at 75 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab238135 was shown to react with PSD95 in wild-type SH-SY5Y cells in Western blot with loss of signal observed in DLG4 knockout cell line Human PSD95 knockout SH-SY5Y cell line ab280043 (DLG4 knockout cell lysate Human PSD95 knockout SH-SY5Y cell lysate ab280102). Wild-type SH-SY5Y and DLG4 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab238135 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (ab238135) at 1/2000 dilution
Lane 1: Wild-type SH-SY5Y cell lysate at 20 µg
Lane 2: DLG4 knockout SH-SY5Y cell lysate at 20 µg
Lane 2: Western blot - Human PSD95 knockout SH-SY5Y cell line (Human PSD95 knockout SH-SY5Y cell line ab280043)
Lane 3: HeLa cell lysate at 20 µg
Lane 4: HL-60 cell lysate at 20 µg
Secondary
All lanes: Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 75 kDa
Western blot - Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (ab238135)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The molecular weight observed is consistent with what has been described in the literature (PMID: 20118925)
Exposure time: 48 seconds
All lanes: Western blot - Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (ab238135) at 1/2000 dilution
Lane 1: Human cerebellum lysate at 20 µg
Lane 2: Human brain lysate at 20 µg
Secondary
All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 80 kDa
Observed band size: 100 kDa, 75 kDa
Immunoprecipitation - Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (ab238135)
PSD95 was immunoprecipitated from 0.35 mg Mouse brain lysate with ab238135 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238135 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain lysate 10μg
Lane 2: ab238135 IP in Mouse brain lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab238135 in Mouse brain lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
All lanes: Immunoprecipitation - Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (ab238135)
Predicted band size: 47 kDa, 80 kDa
Immunohistochemistry (Frozen sections) - Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (ab238135)
Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat retina tissue labeling VSIG4 with ab238135 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (ab238135)
Immunohistochemical analysis of paraffin-embedded Rat retina tissue labeling PSD95 with ab238135 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on outer plexiform layer of rat retina is observed. The section was incubated with ab238135 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins.
Immunohistochemistry (Frozen sections) - Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (ab238135)
Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse retina tissue labeling VSIG4 with ab238135 at 1/100 (5.76 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.
Western blot - Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (ab238135)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The molecular weight observed is consistent with what has been described in the literature (PMID: 20118925).
Exposure time: 15 seconds
All lanes: Western blot - Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (ab238135) at 1/2000 dilution
Lane 1: Mouse brain lysate at 20 µg
Lane 2: Mouse cerebellum lysate at 20 µg
Lane 3: Rat brain lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 80 kDa
Observed band size: 100 kDa, 75 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (ab238135)
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling PSD95 with ab238135 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on basket cells of mouse cerebellum is observed. The section was incubated with ab238135 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins.
Western blot - Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (ab238135)
Western blot: Anti-DLG4 antibody [EPR23124-118] (ab238135) staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab238135 was shown to bind specifically to DLG4. A band was observed at 80 kDa in wild-type U-87 MG cell lysates with no signal observed at this size in DLG4 knockout cell line. To generate this image, wild-type and DLG4 knockout U-87 MG cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker (ab238135) at 1/2000 dilution
Lane 1: Wild-type U-87 MG cell lysate at 20 µg
Lane 2: Western blot - Human DLG4 knockout U-87 MG cell line (Human DLG4 knockout U-87 MG cell line ab306652)
Lane 2: DLG4 knockout U-87 MG cell lysate at 20 µg
Lane 3: Wild-type SH-SY5Y cell lysate at 20 µg
Lane 4: DLG4 knockout SH-SY5Y Human PSD95 knockout SH-SY5Y cell line ab280043 cell lysate at 20 µg
Secondary
Lanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution
Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.