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AB16496

Anti-PSD95 (phospho T19) antibody

3

(3 Reviews)

|

(4 Publications)

Rabbit Polyclonal PSD95 phospho T19 antibody. Suitable for WB and reacts with Mouse samples. Cited in 4 publications.

View Alternative Names

Dlgh4, Psd95, Dlg4, Disks large homolog 4, Postsynaptic density protein 95, Synapse-associated protein 90, PSD-95, SAP-90, SAP90

1 Images
Western blot - Anti-PSD95 (phospho T19) antibody (AB16496)
  • WB

Lab

Western blot - Anti-PSD95 (phospho T19) antibody (AB16496)

In WB, we recommend blocking in milk. Blocking with BSA has been seen to give high background with this antibody.

Lanes 1 and 3:

ab16496 at 10 µg

Lane 2:

ab16496

Lane 1:

Mouse Brain at 10 µg

Lane 2:

Mouse Brain at 10 µg with with modified blocking peptide

Lane 3:

Mouse Brain at 10 µg with with non-modified blocking peptide

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/5000 dilution

Predicted band size: 80 kDa

Observed band size: 90 kDa

true

Exposure time: 20min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB172628

Anti-PSD95 (phospho T19) antibody [EPR2619(2)]

RabMAb|Recombinant

Western blot - Anti-PSD95 (phospho T19) antibody [EPR2619(2)] (AB172628)

  • 5
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Primary Antibodies

AB18258

Anti-PSD95 antibody - Synaptic Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-PSD95 antibody - Synaptic Marker (AB18258)

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Primary Antibodies

AB76127

Anti-PSD95 (phospho S418) antibody [EPR2618Y]

RabMAb|Recombinant

Western blot - Anti-PSD95 (phospho S418) antibody [EPR2618Y] (AB76127)

  • 0
  • 0
Primary Antibodies

AB249719

Anti-PSD95 (phospho T19) antibody [EPR2619(2)] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-PSD95 (phospho T19) antibody [EPR2619(2)] - BSA and Azide free (AB249719)

  • 0
  • 0

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Primary Antibodies

AB32127

Anti-Synaptophysin antibody [YE269] - Synaptic Marker

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Western blot - Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB32127)

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Primary Antibodies

AB108319

Anti-BDNF antibody [EPR1292]

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Western blot - Anti-BDNF antibody [EPR1292] (AB108319)

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Primary Antibodies

AB254349

Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker

BOND RX™ Validated|Lab Essentials|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (AB254349)

  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Phosphorylation of PSD95 dynamically regulates the clustering of PSD95/NMDA receptors at synapses, providing a possible mechanism for rapid changes in density and/or number of receptor at synapses.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Postsynaptic scaffolding protein that plays a critical role in synaptogenesis and synaptic plasticity by providing a platform for the postsynaptic clustering of crucial synaptic proteins (PubMed : 15358775, PubMed : 9853749). Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression (Probable). Under basal conditions, cooperates with FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane through FYN-mediated phosphorylation of ZDHHC5 and its subsequent inhibition of association with endocytic proteins (By similarity).
See full target information Dlg4 phospho T19
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:2849 PubMed33990590

2021

NMDAR-dependent long-term depression is associated with increased short term plasticity through autophagy mediated loss of PSD-95.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Compans,Come Camus,Emmanouela Kallergi,Silvia Sposini,Magalie Martineau,Corey Butler,Adel Kechkar,Remco V Klaassen,Natacha Retailleau,Terrence J Sejnowski,August B Smit,Jean-Baptiste Sibarita,Thomas M Bartol,David Perrais,Vassiliki Nikoletopoulou,Daniel Choquet,Eric Hosy

Frontiers in molecular neuroscience 13:31 PubMed32256312

2020

An Alternative Pin1 Binding and Isomerization Site in the N-Terminus Domain of PSD-95.

Applications

Unspecified application

Species

Unspecified reactive species

Jary Y Delgado

Alcoholism, clinical and experimental research 43:2079-2089 PubMed31403700

2019

Systemic Administration of the Cyclin-Dependent Kinase Inhibitor (S)-CR8 Selectively Reduces Escalated Ethanol Intake in Dependent Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Scott P Goulding,Giordano de Guglielmo,Lieselot L G Carrette,Olivier George,Candice Contet

Journal of neurochemistry 146:133-144 PubMed29337352

2018

Genetic removal of eIF2α kinase PERK in mice enables hippocampal L-LTP independent of mTORC1 activity.

Applications

WB

Species

Mouse

Helena R Zimmermann,Wenzhong Yang,Brenna C Beckelman,Nicole P Kasica,Xueyan Zhou,Lucas Dufresne Galli,Alexey G Ryazanov,Tao Ma
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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