Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (ab316974)

Rabbit Recombinant Monoclonal ompF antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP, I-ELISA, IHC-P and reacts with Transfected cell lysate - Pseudomonas aeruginosa, Transfected cell line - Pseudomonas aeruginosa, Recombinant full length protein - Pseudomonas aeruginosa samples.

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Images

Western blot - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (AB316974), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	ICC/IF	Flow Cyt (Intra)	IP	I-ELISA	IHC-P
Pseudomonas aeruginosa	Predicted	Predicted	Predicted	Predicted	Predicted	Predicted
Recombinant full length protein - Pseudomonas aeruginosa	Not recommended	Not recommended	Not recommended	Not recommended	Tested	Not recommended
Transfected cell line - Pseudomonas aeruginosa	Not recommended	Tested	Tested	Not recommended	Not recommended	Tested
Transfected cell lysate - Pseudomonas aeruginosa	Tested	Not recommended	Not recommended	Tested	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell lysate - Pseudomonas aeruginosa	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Pseudomonas aeruginosa	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line - Pseudomonas aeruginosa, Recombinant full length protein - Pseudomonas aeruginosa	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line - Pseudomonas aeruginosa	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Pseudomonas aeruginosa	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Pseudomonas aeruginosa, Recombinant full length protein - Pseudomonas aeruginosa	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line - Pseudomonas aeruginosa	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Pseudomonas aeruginosa	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Pseudomonas aeruginosa, Recombinant full length protein - Pseudomonas aeruginosa	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell lysate - Pseudomonas aeruginosa	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Pseudomonas aeruginosa	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line - Pseudomonas aeruginosa, Recombinant full length protein - Pseudomonas aeruginosa	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant full length protein - Pseudomonas aeruginosa	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Pseudomonas aeruginosa	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Pseudomonas aeruginosa, Transfected cell line - Pseudomonas aeruginosa	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line - Pseudomonas aeruginosa	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Pseudomonas aeruginosa	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Pseudomonas aeruginosa, Recombinant full length protein - Pseudomonas aeruginosa	Dilution info -	Notes -

Target data

See full target information oprF

Function

Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium.

Alternative names

PA1777, oprF, Outer membrane porin F

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR28629-91
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab316974 is the carrier-free version of Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Pseudomonas Outer Membrane Porin F (OprF) is a protein in Pseudomonas aeruginosa with a molecular mass of approximately 38 kDa. It forms channels across the bacterial outer membrane and allows small hydrophilic molecules to pass functioning as a non-specific diffusion channel. OprF is essential for maintaining the structural integrity of the outer membrane and it is majorly expressed in the outer membrane of Pseudomonas species.

Biological function summary

The Pseudomonas Outer Membrane Porin F plays an important role in nutrient uptake and waste removal. As a part of the outer membrane complex OprF works closely with other porins facilitating the transport of ions and small molecules. It contributes to the permeability of the bacterial membrane which is critical for the bacteria's adaptability and survival in diverse environments.

Pathways

OprF is significant in the general porin-mediated transport pathways that facilitate the exchange of essential nutrients and ions. OprF interacts with other porin proteins forming part of a porous barrier that aligns with the bacteria's need for environmental adaptation. Its role is critical in the context of biofilm formation pathways and antibiotic resistance mechanisms often partnering with OprD porin in these processes.

Associated diseases and disorders

OprF is linked with chronic infections particularly in cystic fibrosis patients where Pseudomonas aeruginosa displays increased pathogenicity. OprF interacts with proteins like AlgC promoting biofilm formation that contributes to the bacterium's resistance to treatment. Its involvement in multidrug resistance also aligns with its role in chronic lung infections complicating the treatment strategies for affected individuals.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Western blot - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (ab316974)
This data was developed using Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973, the same antibody clone in a different buffer formulation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] (Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg with NFDM/TBST
Lane 2: 293T transfected with a Pseudomonas Outer membrane porin F expression vector containing a myc-His-tag®, whole cell lysate at 20 µg with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 38 kDa, 36 kDa
Exposure time: 26s
Immunoprecipitation - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (ab316974)
This data was developed using Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973, the same antibody clone in a different buffer formulation.
Pseudomonas Outer membrane porin F was immunoprecipitated from 0.35 mg 293T(human embryonic kidney epithelial cell) cells transfected with a Pseudomonas Outer membrane porin F expression vector containing a myc-His-tag®, whole cell lysate with Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: 293T(human embryonic kidney epithelial cell) cells transfected with a Pseudomonas Outer membrane porin F expression vector containing a myc-His-tag®, whole cell lysate

Lane 2: Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973 IP in 293T(human embryonic kidney epithelial cell) cells transfected with a Pseudomonas Outer membrane porin F expression vector containing a myc-His-tag®, whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973 in 293T cells transfected with a Pseudomonas Outer membrane porin F expression vector containing a myc-His-tag®, whole cell lysate
All lanes: Immunoprecipitation - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] (Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973) at 1/30 dilution
All lanes: 293T(human embryonic kidney epithelial cell) cells transfected with a Pseudomonas Outer membrane porin F expression vector containing a myc-His-tag®, whole cell lysate at 5 µg with NFDM/TBST
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 10s
Immunocytochemistry/ Immunofluorescence - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (ab316974)
This data was developed using Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Pseudomonas Outer membrane porin F with Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973 at 1/2000 (0.244 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in 293T cells transfected with the Pseudomonas outer membrane porin F vector containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-Myc tag antibody [9E10] ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Indirect ELISA - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (ab316974)
This data was developed using Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Pseudomonas Outer membrane porin F.
Antigen concentration: 1000 ng/ml
Flow Cytometry (Intracellular) - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (ab316974)
This data was developed using Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T (human embryonic kidney epithelial cell) cells transfected with a Pseudomonas Outer membrane porin F expression vector containing a Myc tag (Middle) / 293T cells transfected with an empty expression vector containing a Myc tag (Right) cells labelling Pseudomonas Outer membrane porin F with Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973 at 1/5000 dilution (0.01 ug)/Middle and Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Cells were stained with anti-myc tag conjugated to Alexa Fluor®647.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (ab316974)
This data was developed using Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Pseudomonas Outer membrane porin F expression vector containing a myc-His-tag®. (B) HEK-293T transfected with an empty vector containing a myc-His-tag®. tissue labeling Pseudomonas Outer membrane porin F with Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973 at 1/5000 (0.098 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Pseudomonas Outer membrane porin F expression vector containing a myc-His-tag®, no staining in (B) HEK-293T transfected with an empty vector containing a myc-His-tag®.

The section was incubated with Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (ab316974)
This data was developed using Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Pseudomonas Outer membrane porin F with Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973 at 1/5000 (0.098 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in human cerebrum.

The section was incubated with Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (ab316974)
This data was developed using Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Pseudomonas Outer membrane porin F with Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973 at 1/5000 (0.098 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in rat cerebrum.

The section was incubated with Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (ab316974)
This data was developed using Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Pseudomonas Outer membrane porin F with Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973 at 1/5000 (0.098 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in mouse cerebrum.

The section was incubated with Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] ab316973 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free

Images

Key facts

Immunogen

Reactivity data

WB

TestedTested

PredictedPredicted

Not recommendedNot recommended

ICC/IF

TestedTested

PredictedPredicted

Not recommendedNot recommended

Flow Cyt (Intra)

TestedTested

PredictedPredicted

Not recommendedNot recommended

IP

TestedTested

PredictedPredicted

Not recommendedNot recommended

I-ELISA

TestedTested

PredictedPredicted

Not recommendedNot recommended

IHC-P

TestedTested

PredictedPredicted

Not recommendedNot recommended

Target data

Function

Alternative names

Recommended products

Key facts

Storage

Notes

Supplementary info

Product promise

Tested

Expected

Predicted

Not recommended

9 product images

Western blot - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (ab316974)

Immunoprecipitation - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (ab316974)

Immunocytochemistry/ Immunofluorescence - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (ab316974)

Indirect ELISA - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (ab316974)

Flow Cytometry (Intracellular) - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (ab316974)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (ab316974)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (ab316974)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (ab316974)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (ab316974)

Downloads

Product protocols

Tested
Tested

Predicted
Predicted

Not recommended
Not recommended

Tested
Tested

Predicted
Predicted

Not recommended
Not recommended

Tested
Tested

Predicted
Predicted

Not recommended
Not recommended

Tested
Tested

Predicted
Predicted

Not recommended
Not recommended

Tested
Tested

Predicted
Predicted

Not recommended
Not recommended

Tested
Tested

Predicted
Predicted

Not recommended
Not recommended