Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (AB316974)

This data was developed using ab316973, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Pseudomonas Outer membrane porin F expression vector containing a myc-His-tag®. (B) HEK-293T transfected with an empty vector containing a myc-His-tag®. tissue labeling Pseudomonas Outer membrane porin F with ab316973 at 1/5000 (0.098 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Pseudomonas Outer membrane porin F expression vector containing a myc-His-tag®, no staining in (B) HEK-293T transfected with an empty vector containing a myc-His-tag®.
The section was incubated with ab316973 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (AB316974)

This data was developed using ab316973, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Pseudomonas Outer membrane porin F with ab316973 at 1/5000 (0.098 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human cerebrum.
The section was incubated with ab316973 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (AB316974)

This data was developed using ab316973, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Pseudomonas Outer membrane porin F with ab316973 at 1/2000 (0.244 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells transfected with the Pseudomonas outer membrane porin F vector containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (AB316974)

This data was developed using ab316973, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T (human embryonic kidney epithelial cell) cells transfected with a Pseudomonas Outer membrane porin F expression vector containing a Myc tag (Middle) / 293T cells transfected with an empty expression vector containing a Myc tag (Right) cells labelling Pseudomonas Outer membrane porin F with ab316973 at 1/5000 dilution (0.01 ug)/Middle and Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were stained with anti-myc tag conjugated to Alexa Fluor®647.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (AB316974)

This data was developed using ab316973, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Pseudomonas Outer membrane porin F with ab316973 at 1/5000 (0.098 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in rat cerebrum.
The section was incubated with ab316973 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (AB316974)

This data was developed using ab316973, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Pseudomonas Outer membrane porin F with ab316973 at 1/5000 (0.098 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in mouse cerebrum.
The section was incubated with ab316973 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (AB316974)

This data was developed using ab316973, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab316973 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Pseudomonas Outer membrane porin F.

Antigen concentration : 1000 ng/ml

• IP

Supplier Data

Immunoprecipitation - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (AB316974)

This data was developed using ab316973, the same antibody clone in a different buffer formulation.

Pseudomonas Outer membrane porin F was immunoprecipitated from 0.35 mg 293T(human embryonic kidney epithelial cell) cells transfected with a Pseudomonas Outer membrane porin F expression vector containing a myc-His-tag®, whole cell lysate with ab316973 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316973 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : 293T(human embryonic kidney epithelial cell) cells transfected with a Pseudomonas Outer membrane porin F expression vector containing a myc-His-tag®, whole cell lysate
Lane 2 : ab316973 IP in 293T(human embryonic kidney epithelial cell) cells transfected with a Pseudomonas Outer membrane porin F expression vector containing a myc-His-tag®, whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab316973 in 293T cells transfected with a Pseudomonas Outer membrane porin F expression vector containing a myc-His-tag®, whole cell lysate

All lanes:

Immunoprecipitation - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] (<a href='/en-us/products/primary-antibodies/pseudomonas-outer-membrane-porin-f-antibody-epr28629-91-ab316973'>ab316973</a>) at 1/30 dilution

All lanes:

293T(human embryonic kidney epithelial cell) cells transfected with a Pseudomonas Outer membrane porin F expression vector containing a myc-His-tag®, whole cell lysate at 5 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] - BSA and Azide free (AB316974)

This data was developed using ab316973, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Pseudomonas Outer membrane porin F antibody [EPR28629-91] (<a href='/en-us/products/primary-antibodies/pseudomonas-outer-membrane-porin-f-antibody-epr28629-91-ab316973'>ab316973</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with a Pseudomonas Outer membrane porin F expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 38 kDa,36 kDa

false

Exposure time: 26s