AB183524

Anti-PSF1 antibody

Name: Anti-PSF1 antibody (ab183524) | Abcam
Brand: Abcam Limited
SKU: AB183524
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal PSF1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human GINS1 aa 100 to C-terminus.

View Alternative Names

KIAA0186, PSF1, GINS1, DNA replication complex GINS protein PSF1, GINS complex subunit 1

2 Images

Supplier Data

Immunoprecipitation - Anti-PSF1 antibody (AB183524)

Detection of PSF1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab183524 at 6 μg/mg lysate for IP (Lane 1). For WB detection ab183524 was used at 1 μg/ml. Lane 2 represents control IgG IP.

All lanes:

Immunoprecipitation - Anti-PSF1 antibody (ab183524)

Predicted band size: 23 kDa

true

Exposure time: 30s

Supplier Data

Western blot - Anti-PSF1 antibody (AB183524)

All lanes:

Western blot - Anti-PSF1 antibody (ab183524) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 23 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human GINS1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14691

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common marmoset": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab183524 was affinity purified using an epitope specific to PSF1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks (PubMed : 17417653, PubMed : 28414293). GINS complex is a core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232).

See full target information GINS1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer therapeutics 23:1568-1585 PubMed38982858

2024

Identification of ATP-Competitive Human CMG Helicase Inhibitors for Cancer Intervention that Disrupt CMG-Replisome Function.

Applications

Unspecified application

Species

Unspecified reactive species

Shengyan Xiang,Kendall C Craig,Xingju Luo,Darcy L Welch,Renan B Ferreira,Harshani R Lawrence,Nicholas J Lawrence,Damon R Reed,Mark G Alexandrow

iScience 25:104952 PubMed36065190

2022

GINS1 promotes the proliferation and migration of glioma cells through USP15-mediated deubiquitination of TOP2A.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Yang,Xiaocen Liu,Xiaolong Zhu,Mengying Zhang,Yingying Wang,Mingzhe Ma,Kun Lv

JCI insight 7: PubMed35603789

2022

Hypomorphic GINS3 variants alter DNA replication and cause Meier-Gorlin syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Mary E McQuaid,Kashif Ahmed,Stephanie Tran,Justine Rousseau,Ranad Shaheen,Kristin D Kernohan,Kyoko E Yuki,Prerna Grover,Ema S Dreseris,Sameen Ahmed,Lucie Dupuis,Jennifer Stimec,Mary Shago,Zuhair N Al-Hassnan,Roch Tremblay,Philipp G Maass,Michael D Wilson,Eyal Grunebaum,Kym M Boycott,François-Michel Boisvert,Sateesh Maddirevula,Eissa A Faqeih,Fahad Almanjomi,Zaheer Ullah Khan,Fowzan S Alkuraya,Philippe M Campeau,Peter Kannu,Eric I Campos,Hugo Wurtele

Molecular medicine reports 17:5336-5343 PubMed29363718

2018

Human placenta mesenchymal stem cells suppress airway inflammation in asthmatic rats by modulating Notch signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Li,Tiantian Qu,Lijun Tian,Tingting Han,Yongjun Jin,Yuesi Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PSF1 antibody

Immunoprecipitation - Anti-PSF1 antibody (AB183524)

Western blot - Anti-PSF1 antibody (AB183524)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise