Rabbit Recombinant Monoclonal PSF1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 6 publications.
Images
![Western blot - Anti-PSF1 antibody [EPR13359] (AB181112), expandable thumbnail](https://content.abcam.com/products/images/psf1-antibody-epr13359-ab181112--western-blot-img95154.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PSF1 antibody [EPR13359] (AB181112)")
![Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (AB181112), expandable thumbnail](https://content.abcam.com/products/images/psf1-antibody-epr13359-ab181112--immunocytochemistry-immunofluorescence-img96507.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (AB181112)")
![Western blot - Anti-PSF1 antibody [EPR13359] (AB181112), expandable thumbnail](https://content.abcam.com/products/images/psf1-antibody-epr13359-ab181112--western-blot-img125998.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PSF1 antibody [EPR13359] (AB181112)")
![Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (AB181112), expandable thumbnail](https://content.abcam.com/products/images/psf1-antibody-epr13359-ab181112--immunocytochemistry-immunofluorescence-img13506.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (AB181112)")
![Flow Cytometry (Intracellular) - Anti-PSF1 antibody [EPR13359] (AB181112), expandable thumbnail](https://content.abcam.com/products/images/psf1-antibody-epr13359-ab181112--flow-cytometry-intracellular-img414480.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PSF1 antibody [EPR13359] (AB181112)")
Publications
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- Frontiers in cell and developmental biology 9:7118942021GINS1 Induced Sorafenib Resistance by Promoting Cancer Stem Properties in Human Hepatocellular Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSheng Li,Lina Wu,Hong Zhang,Xijuan Liu,Zilei Wang,Bin Dong,Guang CaoPubMed 34414190
- OncoTargets and therapy 13:11433-114442020Expression Profile of GINS Complex Predicts the Prognosis of Pancreatic Cancer Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFanqin Bu et. al.PubMed 33192076
- Nature communications 11:39512020DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Zhang et. al.PubMed 32769987
- Nature communications 10:24262019The ORC ubiquitin ligase OBI1 promotes DNA replication origin firing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhilippe Coulombe et. al.PubMed 31160578
- Cell reports 27:1794-1808.e52019Remodeling of Interstrand Crosslink Proximal Replisomes Is Dependent on ATR, FANCM, and FANCD2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Huang,Jing Zhang,Marina A Bellani,Durga Pokharel,Julia Gichimu,Ryan C James,Himabindu Gali,Chen Ling,Zhijiang Yan,Dongyi Xu,Junjie Chen,Amom Ruhikanta Meetei,Lei Li,Weidong Wang,Michael M SeidmanPubMed 31067464
- Molecular cell 72:650-660.e82018Nuclear PGK1 Alleviates ADP-Dependent Inhibition of CDC7 to Promote DNA Replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinjian Li,Xu Qian,Hongfei Jiang,Yan Xia,Yanhua Zheng,Jing Li,Bi-Jun Huang,Jing Fang,Chao-Nan Qian,Tao Jiang,Yi-Xin Zeng,Zhimin LuPubMed 30392930
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Tested |
| Mouse | Not recommended | Tested | Expected | Expected |
| Rat | Not recommended | Tested | Expected | Expected |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Species Mouse | Dilution info 1/10000 - 1/50000 | Notes - |
Species Rat | Dilution info 1/10000 - 1/50000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes For unpurified format use at 1/250 - 1/500 dilution. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes For unpurified format use at 1/250 - 1/500 dilution. |
Species Rat | Dilution info 1/100 | Notes For unpurified format use at 1/250 - 1/500 dilution. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks (PubMed:17417653, PubMed:28414293). GINS complex is a core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232).
Alternative names
KIAA0186, PSF1, GINS1, DNA replication complex GINS protein PSF1, GINS complex subunit 1
Recommended products
Rabbit Recombinant Monoclonal PSF1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR13359
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-PSF1 antibody [EPR13359] (ab181112), expandable thumbnail](https://content.abcam.com/products/images/psf1-antibody-epr13359-ab181112--western-blot-img95154.jpg "Western blot - Anti-PSF1 antibody [EPR13359] (ab181112)")
Western blot - Anti-PSF1 antibody [EPR13359] (ab181112)
All lanes: Western blot - Anti-PSF1 antibody [EPR13359] (ab181112) at 1/10000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2: Human fetal heart lysates at 15 µg
Lane 3: Mouse heart lysates at 15 µg
Lane 4: Rat heart lysates at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (ab181112), expandable thumbnail](https://content.abcam.com/products/images/psf1-antibody-epr13359-ab181112--immunocytochemistry-immunofluorescence-img96507.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (ab181112)")
Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (ab181112)
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PSF1 with purified ab181112 at 1/100 dilution (7.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Western blot - Anti-PSF1 antibody [EPR13359] (ab181112), expandable thumbnail](https://content.abcam.com/products/images/psf1-antibody-epr13359-ab181112--western-blot-img125998.jpg "Western blot - Anti-PSF1 antibody [EPR13359] (ab181112)")
Western blot - Anti-PSF1 antibody [EPR13359] (ab181112)
All lanes: Western blot - Anti-PSF1 antibody [EPR13359] (ab181112) at 1/20000 dilution
Lane 1: Jurkat cell lysate at 20 µg
Lane 2: HeLa cell lysate at 20 µg
Lane 3: HepG2 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 23 kDa, 57 kDa
Observed band size: 23 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (ab181112), expandable thumbnail](https://content.abcam.com/products/images/psf1-antibody-epr13359-ab181112--immunocytochemistry-immunofluorescence-img13506.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (ab181112)")
Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (ab181112)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling PSF1 with ab181112 (unpurified) at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
![Flow Cytometry (Intracellular) - Anti-PSF1 antibody [EPR13359] (ab181112), expandable thumbnail](https://content.abcam.com/products/images/psf1-antibody-epr13359-ab181112--flow-cytometry-intracellular-img414480.jpg "Flow Cytometry (Intracellular) - Anti-PSF1 antibody [EPR13359] (ab181112)")
Flow Cytometry (Intracellular) - Anti-PSF1 antibody [EPR13359] (ab181112)
Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling PSF1 with purified ab181112 at 1/70 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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