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AB181112

Anti-PSF1 antibody [EPR13359]

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(7 Publications)

Rabbit Recombinant Monoclonal PSF1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

View Alternative Names

KIAA0186, PSF1, GINS1, DNA replication complex GINS protein PSF1, GINS complex subunit 1

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (AB181112)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (AB181112)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PSF1 with purified ab181112 at 1/100 dilution (7.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Flow Cytometry (Intracellular) - Anti-PSF1 antibody [EPR13359] (AB181112)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PSF1 antibody [EPR13359] (AB181112)

Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling PSF1 with purified ab181112 at 1/70 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (AB181112)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] (AB181112)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling PSF1 with ab181112 (unpurified) at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

Western blot - Anti-PSF1 antibody [EPR13359] (AB181112)
  • WB

Lab

Western blot - Anti-PSF1 antibody [EPR13359] (AB181112)

All lanes:

Western blot - Anti-PSF1 antibody [EPR13359] (ab181112) at 1/10000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

Human fetal heart lysates at 15 µg

Lane 3:

Mouse heart lysates at 15 µg

Lane 4:

Rat heart lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

Western blot - Anti-PSF1 antibody [EPR13359] (AB181112)
  • WB

Supplier Data

Western blot - Anti-PSF1 antibody [EPR13359] (AB181112)

All lanes:

Western blot - Anti-PSF1 antibody [EPR13359] (ab181112) at 1/20000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 23 kDa,57 kDa

Observed band size: 23 kDa

false

  • Carrier free

    Anti-PSF1 antibody [EPR13359] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13359

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p><strong>For unpurified format use at 1/250 - 1/500 dilution.</strong></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p><strong>For unpurified format use at 1/250 - 1/500 dilution.</strong></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p><strong>For unpurified format use at 1/250 - 1/500 dilution.</strong></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks (PubMed : 17417653, PubMed : 28414293). GINS complex is a core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232).
See full target information GINS1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 41:111836 PubMed36516748

2022

Increased replication origin firing links replication stress to whole chromosomal instability in human cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolas Böhly,Ann-Kathrin Schmidt,Xiaoxiao Zhang,Benjamin O Slusarenko,Magdalena Hennecke,Maik Kschischo,Holger Bastians

Frontiers in cell and developmental biology 9:711894 PubMed34414190

2021

GINS1 Induced Sorafenib Resistance by Promoting Cancer Stem Properties in Human Hepatocellular Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Li,Lina Wu,Hong Zhang,Xijuan Liu,Zilei Wang,Bin Dong,Guang Cao

OncoTargets and therapy 13:11433-11444 PubMed33192076

2020

Expression Profile of GINS Complex Predicts the Prognosis of Pancreatic Cancer Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Fanqin Bu,Xiaojian Zhu,Xuan Yi,Chen Luo,Kang Lin,Jinfeng Zhu,Cegui Hu,Zitao Liu,Jiefeng Zhao,Chao Huang,Wenjun Zhang,Jun Huang

Nature communications 11:3951 PubMed32769987

2020

DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Marina A Bellani,Ryan C James,Durga Pokharel,Yongqing Zhang,John J Reynolds,Gavin S McNee,Andrew P Jackson,Grant S Stewart,Michael M Seidman

Nature communications 10:2426 PubMed31160578

2019

The ORC ubiquitin ligase OBI1 promotes DNA replication origin firing.

Applications

Unspecified application

Species

Unspecified reactive species

Philippe Coulombe,Joelle Nassar,Isabelle Peiffer,Slavica Stanojcic,Yvon Sterkers,Axel Delamarre,Stéphane Bocquet,Marcel Méchali

Cell reports 27:1794-1808.e5 PubMed31067464

2019

Remodeling of Interstrand Crosslink Proximal Replisomes Is Dependent on ATR, FANCM, and FANCD2.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Huang,Jing Zhang,Marina A Bellani,Durga Pokharel,Julia Gichimu,Ryan C James,Himabindu Gali,Chen Ling,Zhijiang Yan,Dongyi Xu,Junjie Chen,Amom Ruhikanta Meetei,Lei Li,Weidong Wang,Michael M Seidman

Molecular cell 72:650-660.e8 PubMed30392930

2018

Nuclear PGK1 Alleviates ADP-Dependent Inhibition of CDC7 to Promote DNA Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Xinjian Li,Xu Qian,Hongfei Jiang,Yan Xia,Yanhua Zheng,Jing Li,Bi-Jun Huang,Jing Fang,Chao-Nan Qian,Tao Jiang,Yi-Xin Zeng,Zhimin Lu
View all publications
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Product promise

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