Rabbit Recombinant Monoclonal PSF1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] - BSA and Azide free (AB240240), expandable thumbnail](https://content.abcam.com/products/images/psf1-antibody-epr13359-bsa-and-azide-free-ab240240--immunocytochemistry-immunofluorescence-img96507.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] - BSA and Azide free (AB240240)")
![Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] - BSA and Azide free (AB240240), expandable thumbnail](https://content.abcam.com/products/images/psf1-antibody-epr13359-bsa-and-azide-free-ab240240--immunocytochemistry-immunofluorescence-img13506.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] - BSA and Azide free (AB240240)")
![Flow Cytometry (Intracellular) - Anti-PSF1 antibody [EPR13359] - BSA and Azide free (AB240240), expandable thumbnail](https://content.abcam.com/products/images/psf1-antibody-epr13359-bsa-and-azide-free-ab240240--flow-cytometry-intracellular-img414480.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PSF1 antibody [EPR13359] - BSA and Azide free (AB240240)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Not recommended | Expected | Tested | Tested |
| Mouse | Not recommended | Expected | Predicted | Predicted |
| Rat | Not recommended | Expected | Predicted | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA.
Alternative names
DNA replication complex GINS protein PSF1, GINS complex subunit 1, PSF1, KIAA0186, GINS1
Recommended products
Rabbit Recombinant Monoclonal PSF1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.
Alternative names
DNA replication complex GINS protein PSF1, GINS complex subunit 1, PSF1, KIAA0186, GINS1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR13359
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab240240 is the carrier-free version of Anti-PSF1 antibody [EPR13359] ab181112.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] - BSA and Azide free (ab240240), expandable thumbnail](https://content.abcam.com/products/images/psf1-antibody-epr13359-bsa-and-azide-free-ab240240--immunocytochemistry-immunofluorescence-img96507.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] - BSA and Azide free (ab240240)")
Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] - BSA and Azide free (ab240240)
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PSF1 with purified Anti-PSF1 antibody [EPR13359] ab181112 at 1:100 dilution (7.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PSF1 antibody [EPR13359] ab181112).
![Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] - BSA and Azide free (ab240240), expandable thumbnail](https://content.abcam.com/products/images/psf1-antibody-epr13359-bsa-and-azide-free-ab240240--immunocytochemistry-immunofluorescence-img13506.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] - BSA and Azide free (ab240240)")
Immunocytochemistry/ Immunofluorescence - Anti-PSF1 antibody [EPR13359] - BSA and Azide free (ab240240)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling PSF1 with Anti-PSF1 antibody [EPR13359] ab181112 (unpurified) at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PSF1 antibody [EPR13359] ab181112).
![Flow Cytometry (Intracellular) - Anti-PSF1 antibody [EPR13359] - BSA and Azide free (ab240240), expandable thumbnail](https://content.abcam.com/products/images/psf1-antibody-epr13359-bsa-and-azide-free-ab240240--flow-cytometry-intracellular-img414480.jpg "Flow Cytometry (Intracellular) - Anti-PSF1 antibody [EPR13359] - BSA and Azide free (ab240240)")
Flow Cytometry (Intracellular) - Anti-PSF1 antibody [EPR13359] - BSA and Azide free (ab240240)
Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling PSF1 with purified Anti-PSF1 antibody [EPR13359] ab181112 at 1/70 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PSF1 antibody [EPR13359] ab181112).
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