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AB247361

Anti-PSGL-1 antibody [EPR1967Y] - BSA and Azide free

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(2 Publications)

Rabbit Recombinant Monoclonal PSGL-1 antibody. Carrier free. Suitable for WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 2 publications.

View Alternative Names

CD162, P-selectin glycoprotein ligand 1, PSGL-1, Selectin P ligand, SELPLG

1 Images
Western blot - Anti-PSGL-1 antibody [EPR1967Y] - BSA and Azide free (AB247361)
  • WB

Unknown

Western blot - Anti-PSGL-1 antibody [EPR1967Y] - BSA and Azide free (AB247361)

This data was developed using ab68143, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PSGL-1 antibody [EPR1967Y] (<a href='/en-us/products/primary-antibodies/psgl-1-antibody-epr1967y-ab68143'>ab68143</a>) at 1/500 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

Recombinant PSGL-1 protein at 5ng

Secondary

All lanes:

HRP conjugated Goat anti-rabit at 1/2000 dilution

Predicted band size: 43 kDa

Observed band size: 120 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1967Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab247361 is the carrier-free version of ab68143.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.. (Microbial infection) Acts as a receptor for enterovirus 71.
See full target information SELPLG
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 18:477 PubMed37393232

2023

CircTBX5 knockdown modulates the miR-558/MyD88 axis to alleviate IL-1β-induced inflammation, apoptosis and extracellular matrix degradation in chondrocytes via inactivating the NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wei,Hongjie Mu,Qiaoyi Cui,Peng Yu,Tong Liu,Tao Wang,Lin Sheng

Respiratory research 14:47 PubMed23635004

2013

Adhesion molecules in subjects with COPD and healthy non-smokers: a cross sectional parallel group study.

Applications

IHC-G

Species

Unspecified reactive species

Kristin Blidberg,Lena Palmberg,Anna James,Bo Billing,Elisabeth Henriksson,Ann-Sofie Lantz,Kjell Larsson,Barbro Dahlén
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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