Rabbit Recombinant Monoclonal PSGL-1 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, ICC/IF, IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (AB255290), expandable thumbnail](https://content.abcam.com/products/images/psgl-1-antibody-epr22504-36-bsa-and-azide-free-ab255290--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117864.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (AB255290)")
![Flow Cytometry - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (AB255290), expandable thumbnail](https://content.abcam.com/products/images/psgl-1-antibody-epr22504-36-bsa-and-azide-free-ab255290--flow-cytometry-img67690.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (AB255290)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (AB255290), expandable thumbnail](https://content.abcam.com/products/images/psgl-1-antibody-epr22504-36-bsa-and-azide-free-ab255290--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img28162.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (AB255290)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (AB255290), expandable thumbnail](https://content.abcam.com/products/images/psgl-1-antibody-epr22504-36-bsa-and-azide-free-ab255290--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img85445.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (AB255290)")
![Immunoprecipitation - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (AB255290), expandable thumbnail](https://content.abcam.com/products/images/psgl-1-antibody-epr22504-36-bsa-and-azide-free-ab255290--immunoprecipitation-img29213.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (AB255290)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
5 products for Alternative Product
2 products for Alternative Version
Target data
Function
A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.(Microbial infection) Acts as a receptor for enterovirus 71.
Alternative names
P-selectin glycoprotein ligand 1, PSGL-1, Selectin P ligand, SELPLG
Recommended products
Rabbit Recombinant Monoclonal PSGL-1 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, ICC/IF, IHC-P and reacts with Human samples.
Alternative names
P-selectin glycoprotein ligand 1, PSGL-1, Selectin P ligand, SELPLG
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR22504-36
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab255290 is the carrier-free version of Anti-PSGL-1 antibody [EPR22504-36] ab227836.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290), expandable thumbnail](https://content.abcam.com/products/images/psgl-1-antibody-epr22504-36-bsa-and-azide-free-ab255290--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117864.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PSGL-1 with Anti-PSGL-1 antibody [EPR22504-36] ab227836 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous staining on human tonsil (PMID: 19594630, 16567389). The section was incubated with Anti-PSGL-1 antibody [EPR22504-36] ab227836 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PSGL-1 antibody [EPR22504-36] ab227836).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Flow Cytometry - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290), expandable thumbnail](https://content.abcam.com/products/images/psgl-1-antibody-epr22504-36-bsa-and-azide-free-ab255290--flow-cytometry-img67690.jpg "Flow Cytometry - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)")
Flow Cytometry - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)
Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cell line labeling PSGL-1 using Anti-PSGL-1 antibody [EPR22504-36] ab227836 at 1/500 dilution (Right). Cells were stained with rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left) or Anti-PSGL-1 antibody [EPR22504-36] ab227836 (Right). Then stained with anti-CD3 conjugated to Alexa Fluor® 647. Gated on viable cells. The secondary antibody was a Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed ab150097) at 1/2000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PSGL-1 antibody [EPR22504-36] ab227836).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290), expandable thumbnail](https://content.abcam.com/products/images/psgl-1-antibody-epr22504-36-bsa-and-azide-free-ab255290--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img28162.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling PSGL-1 with Anti-PSGL-1 antibody [EPR22504-36] ab227836 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous staining on infiltrated leukocytes of human colon (PMID: 19594630). The section was incubated with Anti-PSGL-1 antibody [EPR22504-36] ab227836 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PSGL-1 antibody [EPR22504-36] ab227836).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290), expandable thumbnail](https://content.abcam.com/products/images/psgl-1-antibody-epr22504-36-bsa-and-azide-free-ab255290--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img85445.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling PSGL-1 with Anti-PSGL-1 antibody [EPR22504-36] ab227836 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on microglial cells of human cerebrum is observed. The section was incubated with Anti-PSGL-1 antibody [EPR22504-36] ab227836 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PSGL-1 antibody [EPR22504-36] ab227836).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290), expandable thumbnail](https://content.abcam.com/products/images/psgl-1-antibody-epr22504-36-bsa-and-azide-free-ab255290--immunoprecipitation-img29213.jpg "Immunoprecipitation - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)")
Immunoprecipitation - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)
PSGL-1 was immunoprecipitated form 0.35 mg of Jurkat (human T cell leukemia T lymphocyte) whole cell lysate using Anti-PSGL-1 antibody [EPR22504-36] ab227836 at 1/30 dilution. Western blot was performed on the immunoprecipitate using Anti-PSGL-1 antibody [EPR22504-36] ab227836 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used as the secondary at a 1/1000 dilution.
Lane 1: Jurkat whole cell lysate μg (input).
Lane 2: Anti-PSGL-1 antibody [EPR22504-36] ab227836 IP in jurkat whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PSGL-1 antibody [EPR22504-36] ab227836 in Jurkat whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PSGL-1 antibody [EPR22504-36] ab227836).
The expression profile observed is consistent with what has been described in the literature (PMID: 9660879; PMID: 28515724). PSGL-1(P-selectin is the P-selectin glycoprotein ligand-1) is a 125KD glycoprtein and the 250KD band represents the dimer.
All lanes: Immunoprecipitation - Anti-PSGL-1 antibody [EPR22504-36] (Anti-PSGL-1 antibody [EPR22504-36] ab227836)
Predicted band size: 43 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290), expandable thumbnail](https://content.abcam.com/products/images/psgl-1-antibody-epr22504-36-bsa-and-azide-free-ab255290--immunocytochemistry-immunofluorescence-img18927.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)")
Immunocytochemistry/ Immunofluorescence - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (human T cell leukemia T lymphocyte) cells stained for PSGL-1 using Anti-PSGL-1 antibody [EPR22504-36] ab227836 (Green) at 1/50 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. Confocal image showing membranous staining in Jurkat cells. The nuclear counterstain was DAPI (Blue). Tubulin was stained using the Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PSGL-1 antibody [EPR22504-36] ab227836).
![Immunocytochemistry/ Immunofluorescence - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290), expandable thumbnail](https://content.abcam.com/products/images/psgl-1-antibody-epr22504-36-bsa-and-azide-free-ab255290--immunocytochemistry-immunofluorescence-img18927.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PSGL-1 antibody [EPR22504-36] - BSA and Azide free (ab255290)")
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