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Rabbit Recombinant Monoclonal PSGL-1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt and reacts with Transfected cell line - Mouse, Mouse, Rat samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow CytIP
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Tested
Not recommended
Rat
Tested
Tested
Expected
Expected
Not recommended
Transfected cell line - Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Transfected cell line - Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human, Transfected cell line - Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell line - Mouse, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell line - Mouse, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Transfected cell line - Mouse, Human

Dilution info

-

Notes

-

Target data

Function

A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E- and P-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.

Alternative names

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Rabbit Recombinant Monoclonal PSGL-1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt and reacts with Transfected cell line - Mouse, Mouse, Rat samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR28170-27

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

  • Flow Cytometry - Anti-PSGL-1 antibody [EPR28170-27] (ab316113), expandable thumbnail

    Flow Cytometry - Anti-PSGL-1 antibody [EPR28170-27] (ab316113)

    Flow cytometric analysis of Mouse splenocytes cells labelling PSGL-1 with ab316113 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

    Gated on viable cells. Cells are co-stained with CD3 conjugated to Alexa Fluor®647.

  • Immunocytochemistry/ Immunofluorescence - Anti-PSGL-1 antibody [EPR28170-27] (ab316113), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PSGL-1 antibody [EPR28170-27] (ab316113)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocyte cells labelling PSGL-1 with ab316113 at 1/50 (10.08 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).

    Confocal image showing membrane staining in a subset of mouse splenocytes.

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Anti-rat CD3 mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain at 1/100 (5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR28170-27] (ab316113), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR28170-27] (ab316113)

    Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling PSGL-1 with ab316113 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: weak staining on rat skeletal muscle. The section was incubated with ab316113 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR28170-27] (ab316113), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR28170-27] (ab316113)

    Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling PSGL-1 with ab316113 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: weak staining on mouse skeletal muscle (PMID: 8639776). The section was incubated with ab316113 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR28170-27] (ab316113), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR28170-27] (ab316113)

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse PSGL-1 expression vector containing a Myc-His tag. (B) HEK-293T transfected with empty vector containing a Myc-His tag. tissue labeling PSGL-1 with ab316113 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on HEK-293T transfected with a Myc-His-tagged mouse PSGL-1 construct (image A). No staining on HEK-293T transfected with empty plasmid (image B). The section was incubated with ab316113 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR28170-27] (ab316113), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR28170-27] (ab316113)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling PSGL-1 with ab316113 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat spleen. The section was incubated with ab316113 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR28170-27] (ab316113), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR28170-27] (ab316113)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling PSGL-1 with ab316113 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on immune cells of rat colon. The section was incubated with ab316113 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR28170-27] (ab316113), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR28170-27] (ab316113)

    Immunohistochemical analysis of paraffin-embedded Mouse glioblastoma tissue labeling PSGL-1 with ab316113 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse glioblastoma. The section was incubated with ab316113 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR28170-27] (ab316113), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR28170-27] (ab316113)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling PSGL-1 with ab316113 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse spleen. The section was incubated with ab316113 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR28170-27] (ab316113), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSGL-1 antibody [EPR28170-27] (ab316113)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling PSGL-1 with ab316113 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on immune cells of mouse colon. The section was incubated with ab316113 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-PSGL-1 antibody [EPR28170-27] (ab316113), expandable thumbnail

    Western blot - Anti-PSGL-1 antibody [EPR28170-27] (ab316113)

    Low expression: skeletal muscle, kidney (PMID 8639776).

    The molecular weight observed is consistent with what has been described in the literature (PMID: 18519846, 9660879).

    PSGL-1(P-selectin is the P-selectin glycoprotein ligand-1) is a 125KD glycoprotein and the 250KD band represents the dimer.

    The identity of the bands below 75 kDa are unknown.

    In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-PSGL-1 antibody [EPR28170-27] (AB316113) at 1/1000 dilution

    Lane 1: Rat spleen tissue lysate at 20 µg

    Lane 2: Rat lymph node tissue lysate at 20 µg

    Lane 3: Rat skeletal muscle tissue lysate at 20 µg

    Lane 4: Rat kidney tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Observed band size: 125 kDa, 250 kDa, 124 kDa

    Exposure time: 103s

  • Western blot - Anti-PSGL-1 antibody [EPR28170-27] (ab316113), expandable thumbnail

    Western blot - Anti-PSGL-1 antibody [EPR28170-27] (ab316113)

    Low expression: EL4.IL-2 (PMID: 34090013), NIH/3T3; skeletal muscle (PMID 8639776), kidney (PMID 8639776).

    The molecular weight observed is consistent with what has been described in the literature (PMID: 18519846, 9660879).

    PSGL-1(P-selectin is the P-selectin glycoprotein ligand-1) is a 125KD glycoprotein and the 250KD band represents the dimer.

    The identity of the bands below 75 kDa are unknown.

    In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

    Exposure time: Lane 1-4 : 81 seconds; Lane 5-9 : 10 seconds

    All lanes: Western blot - Anti-PSGL-1 antibody [EPR28170-27] (AB316113) at 1/1000 dilution

    Lane 1: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 2: A20 (mouse reticum sarcoma B lymphocyte) whole cell lysate at 20 µg

    Lane 3: EL4.IL-2 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

    Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 5: Mouse spleen tissue lysate at 20 µg

    Lane 6: Mouse lymph node tissue lysate at 20 µg

    Lane 7: Mouse thymus tissue lysate at 20 µg

    Lane 8: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 9: Mouse kidney tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Observed band size: 125 kDa, 250 kDa, 124 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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