Rabbit Recombinant Monoclonal PSIP1/LEDGF antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: PBS
IHC-P | ICC/IF | IP | Flow Cyt | WB | |
---|---|---|---|---|---|
Human | Tested | Tested | Not recommended | Not recommended | Tested |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.
DFS70, LEDGF, PSIP2, PSIP1, PC4 and SFRS1-interacting protein, CLL-associated antigen KW-7, Dense fine speckles 70 kDa protein, Lens epithelium-derived growth factor, Transcriptional coactivator p75/p52, DFS 70
Rabbit Recombinant Monoclonal PSIP1/LEDGF antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: PBS
ab249943 is the carrier-free version of Anti-PSIP1/LEDGF antibody [EPR11890] ab177159.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using the same antibody clone in a different buffer formulation (Anti-PSIP1/LEDGF antibody [EPR11890] ab177159).
Lanes 1-3: Merged signal (red and green). Green - Anti-PSIP1/LEDGF antibody [EPR11890] ab177159 observed at 75 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
Anti-PSIP1/LEDGF antibody [EPR11890] ab177159 Anti-PSIP1/LEDGF antibody [EPR11890] was shown to specifically react with PSIP1/LEDGF in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human PSIP1 (LEDGF) knockout HEK-293T cell line ab266464 (knockout cell lysate Human PSIP1 (LEDGF) knockout HEK-293T cell lysate ab258144) was used. Wild-type and PSIP1/LEDGF knockout samples were subjected to SDS-PAGE. Anti-PSIP1/LEDGF antibody [EPR11890] ab177159 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (Anti-PSIP1/LEDGF antibody [EPR11890] ab177159) at 1/1000 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: PSIP1 knockout HEK293T cell lysate at 20 µg
Lane 2: Western blot - Human PSIP1 (LEDGF) knockout HEK-293T cell line (Human PSIP1 (LEDGF) knockout HEK-293T cell line ab266464)
Lane 3: Jurkat cell lysate at 20 µg
All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 60 kDa
Observed band size: 75 kDa
This data was developed using Anti-PSIP1/LEDGF antibody [EPR11890] ab177159, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of K562 cells labeling PSIP1/LEDGF with Anti-PSIP1/LEDGF antibody [EPR11890] ab177159 at 1/100 dilution.
This data was developed using Anti-PSIP1/LEDGF antibody [EPR11890] ab177159, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling PSIP1/LEDGF with Anti-PSIP1/LEDGF antibody [EPR11890] ab177159 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using Anti-PSIP1/LEDGF antibody [EPR11890] ab177159, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PSIP1/LEDGF with Anti-PSIP1/LEDGF antibody [EPR11890] ab177159 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using Anti-PSIP1/LEDGF antibody [EPR11890] ab177159, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (Anti-PSIP1/LEDGF antibody [EPR11890] ab177159) at 1/1000 dilution
Lane 1: Jurkat cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: K562 cell lysate at 10 µg
All lanes: Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 60 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com