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AB76104

Anti-PSMA antibody [EP3253]

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(6 Publications)

Rabbit Recombinant Monoclonal PSMA antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.

View Alternative Names

FOLH, NAALAD1, PSM, PSMA, GIG27, FOLH1, Glutamate carboxypeptidase 2, Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I, Prostate-specific membrane antigen, Pteroylpoly-gamma-glutamate carboxypeptidase, FGCP, GCPII, mGCP, NAALADase I

4 Images
Flow Cytometry (Intracellular) - Anti-PSMA antibody [EP3253] (AB76104)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PSMA antibody [EP3253] (AB76104)

Intracellular Flow Cytometry analysis of LNCaP (Human prostatecarcinoma epithelial cell) cells labeling PSMA with Purified ab76104 at 1/20 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-PSMA antibody [EP3253] (AB76104)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PSMA antibody [EP3253] (AB76104)

Immunocytochemistry/ Immunofluorescence analysis of LNCaP (Human prostatecarcinoma epithelial cell) cells labeling PSMA with Purified ab76104 at 1/50 (3.36 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunoprecipitation - Anti-PSMA antibody [EP3253] (AB76104)
  • IP

Unknown

Immunoprecipitation - Anti-PSMA antibody [EP3253] (AB76104)

Purified ab76104 at 1/20 dilution (1ug) immunoprecipitating PSMA in LNCaP whole cell lysate.
Lane 1 (input) : LNCaP (Human prostatecarcinoma epithelial cell) whole cell lysate (10μg)
Lane 2 (+) : ab76104 + LNCaP whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab76104 in LNCaP whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 110 kDa

All lanes:

Immunoprecipitation - Anti-PSMA antibody [EP3253] (ab76104)

Predicted band size: 84 kDa

false

Western blot - Anti-PSMA antibody [EP3253] (AB76104)
  • WB

Unknown

Western blot - Anti-PSMA antibody [EP3253] (AB76104)

Blocking/Diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-PSMA antibody [EP3253] (ab76104) at 1/1000 dilution

All lanes:

LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 84 kDa

Observed band size: 110 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP3253

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression.. Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
See full target information FOLH1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2401095 PubMed38946578

2024

Genetically Engineered Membrane-Coated Nanoparticles for Enhanced Prostate-Specific Membrane Antigen Targeting and Ferroptosis Treatment of Castration-Resistant Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Li,Hongji Li,Keying Zhang,Chao Xu,Jingwei Wang,Zeyu Li,Yike Zhou,Shaojie Liu,Xiaolong Zhao,Zhengxuan Li,Fa Yang,Wei Hu,Yuming Jing,Peng Wu,Jingliang Zhang,Changhong Shi,Rui Zhang,Wenkai Jiang,Nianzeng Xing,Weihong Wen,Donghui Han,Weijun Qin

Journal of immunology research 2023:3804605 PubMed37767202

2023

Super Enhancer Regulatory Gene FYB1 Promotes the Progression of T Cell Acute Lymphoblastic Leukemia by Activating IGLL1.

Applications

Unspecified application

Species

Unspecified reactive species

Kunlong Zhang,Jun Lu,Fang Fang,Yongping Zhang,Juanjuan Yu,Yanfang Tao,Wenyuan Liu,Lihui Lu,Zimu Zhang,Xinran Chu,Jianwei Wang,Xiaolu Li,Yuanyuan Tian,Zhiheng Li,Qian Li,Xu Sang,Li Ma,Ningling Wang,Jian Pan,Shaoyan Hu

Small (Weinheim an der Bergstrasse, Germany) 19:e2204956 PubMed36840671

2023

Nanoparticles Targeted to Fibroblast Activation Protein Outperform PSMA for MRI Delineation of Primary Prostate Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole Dmochowska,Valentina Milanova,Ramesh Mukkamala,Kwok Keung Chow,Nguyen T H Pham,Madduri Srinivasarao,Lisa M Ebert,Timothy Stait-Gardner,Hien Le,Anil Shetty,Melanie Nelson,Philip S Low,Benjamin Thierry

Cancers 14: PubMed35884424

2022

Isolation of Circulating Tumor Cells from Seminal Fluid of Patients with Prostate Cancer Using Inertial Microfluidics.

Applications

Unspecified application

Species

Unspecified reactive species

Alexey S Rzhevskiy,Alina Y Kapitannikova,Steven A Vasilescu,Tamilla A Karashaeva,Sajad Razavi Bazaz,Mark S Taratkin,Dmitry V Enikeev,Vladimir Y Lekarev,Evgeniy V Shpot,Denis V Butnaru,Sergey M Deyev,Jean Paul Thiery,Andrei V Zvyagin,Majid Ebrahimi Warkiani

Gastro hep advances 1:631-639 PubMed35844243

2022

Imaging of Tumor-Associated Vascular Prostate-Specific Membrane Antigen in Woodchuck Model of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Olga Sergeeva,Yifan Zhang,Willian Julian,Arun Sasikumar,Amad Awadallah,Jonathan Kenyon,Wuxian Shi,Maxim Sergeev,Steve Huang,Sandra Sexton,Renuka Iyer,Wei Xin,Norbert Avril,Ernest Ricky Chan,Zhenghong Lee

Frontiers in oncology 11:771036 PubMed34869004

2021

Prognostic Value of Vascular-Expressed PSMA and CD248 in Urothelial Carcinoma of the Bladder.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Li,Keying Zhang,Fa Yang,Dian Jiao,Mingyang Li,Xiaolong Zhao,Chao Xu,Shaojie Liu,Hongji Li,Shengjia Shi,Bo Yang,Lijun Yang,Donghui Han,Weihong Wen,Weijun Qin
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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