Rabbit Recombinant Monoclonal PSMA antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.
Images
![Immunoprecipitation - Anti-PSMA antibody [EPR6253] - BSA and Azide free (AB226141), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-epr6253-bsa-and-azide-free-ab226141--immunoprecipitation-img32369.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PSMA antibody [EPR6253] - BSA and Azide free (AB226141)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (AB226141), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-epr6253-bsa-and-azide-free-ab226141--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img27675.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (AB226141)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (AB226141), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-epr6253-bsa-and-azide-free-ab226141--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103738.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (AB226141)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (AB226141), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-epr6253-bsa-and-azide-free-ab226141--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111703.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (AB226141)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (AB226141), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-epr6253-bsa-and-azide-free-ab226141--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57116.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (AB226141)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Expected | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
10 products for Alternative Product
2 products for Alternative Version
Target data
Function
Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression.Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
Alternative names
Glutamate carboxypeptidase 2, Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I, Prostate-specific membrane antigen, Pteroylpoly-gamma-glutamate carboxypeptidase, FGCP, GCPII, mGCP, NAALADase I, PSM, PSMA, FOLH1, FOLH, NAALAD1, PSM, PSMA, GIG27
Recommended products
Rabbit Recombinant Monoclonal PSMA antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR6253
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab226141 is the carrier-free version of Anti-PSMA antibody [EPR6253] ab133579.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunoprecipitation - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-epr6253-bsa-and-azide-free-ab226141--immunoprecipitation-img32369.jpg "Immunoprecipitation - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)")
Immunoprecipitation - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
Primary: Anti-PSMA antibody [EPR6253] ab133579, 1/50 dilution
Dilution buffer: NFDM/TBST, 5%
Sample: LNCaP
Secondary: HRP goat anti-rabbit (H+L), 1/1000 dilutionThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PSMA antibody [EPR6253] ab133579).
All lanes: Immunoprecipitation - Anti-PSMA antibody [EPR6253] (Anti-PSMA antibody [EPR6253] ab133579) at 1/50 dilution
All lanes: LNCaP
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 84 kDa
Observed band size: 110 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-epr6253-bsa-and-azide-free-ab226141--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img27675.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
This IHC data was generated using the same anti-PSMA antibody clone, EPR6253, in a different buffer formulation (cat# Anti-PSMA antibody [EPR6253] ab133579).
Immunohistochemical staining of paraffin embedded human prostate cancer with purified Anti-PSMA antibody [EPR6253] ab133579 at a working dilution of 1 in 300. HRP polymer for rabbit IgG was used as the secondary antibody, and the sample was counter stained with hematoxylin.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-epr6253-bsa-and-azide-free-ab226141--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103738.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
Unpurified Anti-PSMA antibody [EPR6253] ab133579 showing positive staining in Prostatic carcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PSMA antibody [EPR6253] ab133579).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-epr6253-bsa-and-azide-free-ab226141--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111703.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
Unpurified Anti-PSMA antibody [EPR6253] ab133579 showing positive staining in Normal prostate tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PSMA antibody [EPR6253] ab133579).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-epr6253-bsa-and-azide-free-ab226141--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57116.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
Unpurified Anti-PSMA antibody [EPR6253] ab133579 showing negative staining in Thyroid gland carcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PSMA antibody [EPR6253] ab133579).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-epr6253-bsa-and-azide-free-ab226141--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119817.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
Unpurified Anti-PSMA antibody [EPR6253] ab133579 showing negative staining in Normal colon tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PSMA antibody [EPR6253] ab133579).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-epr6253-bsa-and-azide-free-ab226141--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130567.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
Unpurified Anti-PSMA antibody [EPR6253] ab133579 showing negative staining in Breast carcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PSMA antibody [EPR6253] ab133579).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-epr6253-bsa-and-azide-free-ab226141--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134598.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
Unpurified Anti-PSMA antibody [EPR6253] ab133579 showing negative staining in Normal spleen tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PSMA antibody [EPR6253] ab133579).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-epr6253-bsa-and-azide-free-ab226141--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119817.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-epr6253-bsa-and-azide-free-ab226141--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130567.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-epr6253-bsa-and-azide-free-ab226141--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134598.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)")
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