JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB268182

Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Monoclonal PSMA antibody. Carrier free. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FOLH1 aa 1-200.

View Alternative Names

FOLH, NAALAD1, PSM, PSMA, GIG27, FOLH1, Glutamate carboxypeptidase 2, Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I, Prostate-specific membrane antigen, Pteroylpoly-gamma-glutamate carboxypeptidase, FGCP, GCPII, mGCP, NAALADase I

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (AB268182)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (AB268182)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for PSMA using ab268061 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268061)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (AB268182)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (AB268182)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for PSMA using ab268061 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268061)

Western blot - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (AB268182)
  • WB

Unknown

Western blot - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (AB268182)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268182)

All lanes:

Western blot - Anti-PSMA antibody [FOLH1/3734] (<a href='/en-us/products/primary-antibodies/psma-antibody-folh1-3734-ab268061'>ab268061</a>) at 2 µg/mL

All lanes:

LNCaP (human prostate cancer cell line) cell lysate

Predicted band size: 84 kDa

false

Protein Array - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (AB268182)
  • Protein Array

Unknown

Protein Array - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (AB268182)

Protein Array containing >21,000 full-length human proteins using ab268061.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268061)

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

FOLH1/3734

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

Protein Array, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human FOLH1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q04609

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

Product details

ab268182 is the carrier-free version of ab268061.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from bioreactor concentrate.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression.. Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
See full target information FOLH1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com