Mouse Monoclonal PSMA antibody. Carrier free. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FOLH1 aa 1-200.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (AB268182), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-folh1-3734-bsa-and-azide-free-ab268182--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110990.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (AB268182)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (AB268182), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-folh1-3734-bsa-and-azide-free-ab268182--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42469.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (AB268182)")
![Western blot - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (AB268182), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-folh1-3734-bsa-and-azide-free-ab268182--western-blot-img27667.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (AB268182)")
![Protein Array - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (AB268182), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-folh1-3734-bsa-and-azide-free-ab268182--protein-array-img1220.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (AB268182)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human FOLH1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q04609
Reactivity data
Select an application| WB | Protein Array | IHC-P | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression. Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
Alternative names
FOLH, NAALAD1, PSM, PSMA, GIG27, FOLH1, Glutamate carboxypeptidase 2, Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I, Prostate-specific membrane antigen, Pteroylpoly-gamma-glutamate carboxypeptidase, FGCP, GCPII, mGCP, NAALADase I
Recommended products
Mouse Monoclonal PSMA antibody. Carrier free. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FOLH1 aa 1-200.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human FOLH1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q04609
- Carrier free
- Yes
- Clone number
- FOLH1/3734
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from bioreactor concentrate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab268182 is the carrier-free version of Anti-PSMA antibody [FOLH1/3734] ab268061.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (ab268182), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-folh1-3734-bsa-and-azide-free-ab268182--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110990.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (ab268182)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (ab268182)
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for PSMA using Anti-PSMA antibody [FOLH1/3734] ab268061 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-PSMA antibody [FOLH1/3734] ab268061)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (ab268182), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-folh1-3734-bsa-and-azide-free-ab268182--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42469.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (ab268182)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (ab268182)
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for PSMA using Anti-PSMA antibody [FOLH1/3734] ab268061 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-PSMA antibody [FOLH1/3734] ab268061)
![Western blot - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (ab268182), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-folh1-3734-bsa-and-azide-free-ab268182--western-blot-img27667.jpg "Western blot - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (ab268182)")
Western blot - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (ab268182)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268182)
All lanes: Western blot - Anti-PSMA antibody [FOLH1/3734] (Anti-PSMA antibody [FOLH1/3734] ab268061) at 2 µg/mL
All lanes: LNCaP (human prostate cancer cell line) cell lysate
Predicted band size: 84 kDa
![Protein Array - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (ab268182), expandable thumbnail](https://content.abcam.com/products/images/psma-antibody-folh1-3734-bsa-and-azide-free-ab268182--protein-array-img1220.jpg "Protein Array - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (ab268182)")
Protein Array - Anti-PSMA antibody [FOLH1/3734] - BSA and Azide free (ab268182)
Protein Array containing >21,000 full-length human proteins using Anti-PSMA antibody [FOLH1/3734] ab268061.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-PSMA antibody [FOLH1/3734] ab268061)
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