AB180784

Anti-PSMA3 antibody

Name: Anti-PSMA3 antibody (ab180784) | Abcam
Brand: Abcam Limited
SKU: AB180784

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(1 Publication)

Rabbit Polyclonal PSMA3 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PSMA3.

View Alternative Names

HC8, PSC8, PSMA3, Proteasome subunit alpha type-3, Macropain subunit C8, Multicatalytic endopeptidase complex subunit C8, Proteasome component C8, Proteasome subunit alpha-7, alpha-7

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-PSMA3 antibody (AB180784)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PSMA3 antibody (AB180784)

Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab180784. Blue DAPI for nuclear staining.

Supplier Data

Immunoprecipitation - Anti-PSMA3 antibody (AB180784)

Immunoprecipitation analysis of 200 μg extracts of HL-60 cells, using 3 μg of ab180784. Western blot was performed from the immunoprecipitate using ab180784 at a dilition of 1 : 1000.

All lanes:

Immunoprecipitation - Anti-PSMA3 antibody (ab180784)

Predicted band size: 28 kDa

false

Supplier Data

Western blot - Anti-PSMA3 antibody (AB180784)

All lanes:

Western blot - Anti-PSMA3 antibody (ab180784) at 1/500 dilution

Lane 1:

JAR cell extracts

Lane 2:

A549 cell extracts

Lane 3:

PC12 cell extracts

Predicted band size: 28 kDa

false

Supplier Data

Western blot - Anti-PSMA3 antibody (AB180784)

All lanes:

Western blot - Anti-PSMA3 antibody (ab180784) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 25 µg

Lane 2:

DU145 cell lysate at 25 µg

Lane 3:

HL-60 cell lysate at 25 µg

Lane 4:

PC-3 cell lysate at 25 µg

Lane 5:

Mouse liver lysate at 25 µg

Lane 6:

Mouse spleen lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PSMA3.

P25788

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/1000", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.

See full target information PSMA3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Arteriosclerosis, thrombosis, and vascular biology 41:668-682 PubMed33297751

2020

Targeting Thymidine Phosphorylase With Tipiracil Hydrochloride Attenuates Thrombosis Without Increasing Risk of Bleeding in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Belcher,Abu Hasanat Md Zulfiker,Oliver Qiyue Li,Hong Yue,Anirban Sen Gupta,Wei Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PSMA3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PSMA3 antibody (AB180784)

Immunoprecipitation - Anti-PSMA3 antibody (AB180784)

Western blot - Anti-PSMA3 antibody (AB180784)

Western blot - Anti-PSMA3 antibody (AB180784)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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