Anti-PSMA4 antibody [EPR5831(2)]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA4 antibody [EPR5831(2)] (AB191403)

Immunohistochemical analysis of paraffin-embedded, Human papillary adenocarcinoma of thyroid tissue labeling PSMA4 with ab191403 at a 1/2000 dilution (1μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PSMA4 antibody [EPR5831(2)] (AB191403)

Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling PSMA4 with ab191403 at a 1/500 dilution ( 4 ug/ml). As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/200 dilution. In blue DAPI staining. In the negative controls cells were treated with anti-PSMA4 at a 1/500 dilution as primary antibody and goat anti-mouse IgG (Alexa Fluor®594) at a 1/400 dilution as secondary antibody.

• IP

Lab

Immunoprecipitation - Anti-PSMA4 antibody [EPR5831(2)] (AB191403)

PSMA4 was immunoprecipitated from 0.35mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab191403 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab191403 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate, 10µg Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab191403 in K-562 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Observed MV : 29kDa. Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-PSMA4 antibody [EPR5831(2)] (ab191403) at 1/1000 dilution

Lane 1:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 10 µg

Lane 2:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab191403 in K-562 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Exposure time: 180s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA4 antibody [EPR5831(2)] (AB191403)

Immunohistochemical analysis of paraffin-embedded, mouse liver tissue labeling PSMA4 with ab191403 at a 1/2000 dilution (1μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-PSMA4 antibody [EPR5831(2)] (AB191403)

All lanes:

Western blot - Anti-PSMA4 antibody [EPR5831(2)] (ab191403) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

JAR cell lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

true

• WB

Supplier Data

Western blot - Anti-PSMA4 antibody [EPR5831(2)] (AB191403)

All lanes:

Western blot - Anti-PSMA4 antibody [EPR5831(2)] (ab191403) at 1/1000 dilution

Lane 1:

rat C6 cell lysate at 10 µg

Lane 2:

mouse Raw 264.7 cell lysate at 10 µg

Lane 3:

rat PC12 cell lysate at 10 µg

Lane 4:

mouse NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

true