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AB133502

Anti-PSMA7/HSPC antibody [EPR5836]

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(4 Publications)

Rabbit Recombinant Monoclonal PSA7 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

HSPC, PSMA7, Proteasome subunit alpha type-7, Proteasome subunit RC6-1, Proteasome subunit XAPC7, Proteasome subunit alpha-4, alpha-4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA7/HSPC antibody [EPR5836] (AB133502)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA7/HSPC antibody [EPR5836] (AB133502)

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling PSMA7/HSPC using ab133502 antibody at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-PSMA7/HSPC antibody [EPR5836] (AB133502)
  • WB

Unknown

Western blot - Anti-PSMA7/HSPC antibody [EPR5836] (AB133502)

All lanes:

Western blot - Anti-PSMA7/HSPC antibody [EPR5836] (ab133502) at 1/1000 dilution

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 28 kDa

Observed band size: 27 kDa

false

OI-RD Scanning - Anti-PSMA7/HSPC antibody [EPR5836] (AB133502)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-PSMA7/HSPC antibody [EPR5836] (AB133502)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5836

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Inhibits the transactivation function of HIF-1A under both normoxic and hypoxia-mimicking conditions. The interaction with EMAP2 increases the proteasome-mediated HIF-1A degradation under the hypoxic conditions. Plays a role in hepatitis C virus internal ribosome entry site-mediated translation. Mediates nuclear translocation of the androgen receptor (AR) and thereby enhances androgen-mediated transactivation. Promotes MAVS degradation and thereby negatively regulates MAVS-mediated innate immune response.
See full target information PSMA7
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed40091835

2025

Targeting ubiquitin-independent proteasome with small molecule increases susceptibility in pan-KRAS-mutant cancer models.

Applications

Unspecified application

Species

Unspecified reactive species

Shihui Shen,Qiansen Zhang,Yuhan Wang,Hui Chen,Shuangming Gong,Yun Liu,Conghao Gai,Hansen Chen,Enhao Zhu,Bo Yang,Lin Liu,Siyuan Cao,Mengting Zhao,Wenjie Ren,Mengjuan Li,Zhuoya Peng,Lu Zhang,Shaoying Zhang,Juwen Shen,Bianhong Zhang,Patrick Kh Lee,Kun Li,Lei Li,Huaiyu Yang

In vivo (Athens, Greece) 37:1584-1592 PubMed37369480

2023

Proteasome Subunit Alpha Type-7 Expression Suppresses Cutaneous Squamous Cell Carcinoma Progression by Inhibiting Cancer-associated Cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangshu Xu,Meiling Pei,Ki-Yeol Kim,Haoran Xi,Sang Gyun Lee,Kee Yang Chung,Mi Ryung Roh,Zhehu Jin

The Journal of clinical investigation 128:3630-3641 PubMed29809168

2018

Androgen receptor polyglutamine expansion drives age-dependent quality control defects and muscle dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Samir R Nath,Zhigang Yu,Theresa A Gipson,Gregory B Marsh,Eriko Yoshidome,Diane M Robins,Sokol V Todi,David E Housman,Andrew P Lieberman

Journal of cellular physiology 234:5851-5862 PubMed29247526

2017

Effects of shRNA-mediated silencing of PSMA7 on cell proliferation and vascular endothelial growth factor expression via the ubiquitin-proteasome pathway in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chen-Chen Ren,Li Yang,Ling Liu,Yan-Nan Chen,Guo-Mei Cheng,Xiao-An Zhang,Hui Liu
View all publications
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Product promise

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