Rabbit Polyclonal PSB1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PSMB1.
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Publications
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- Journal of virology 97:e01660222023PSMB1 Inhibits the Replication of Porcine Reproductive and Respiratory Syndrome Virus by Recruiting NBR1 To Degrade Nonstructural Protein 12 by Autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiwei Li,Yuanzhe Bai,Yanjun Zhou,Yifeng Jiang,Wu Tong,Guoxin Li,Haihong Zheng,Fei Gao,Guangzhi TongPubMed 36602366
- American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 20:399-4102019Proteasomal adaptations underlying carfilzomib-resistance in human bone marrow plasma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesE Steve Woodle,Simon Tremblay,Paul Brailey,Alin Girnita,Rita R Alloway,Bruce Aronow,Nupur Dasgupta,Frederic Ebstein,Peter-Michael Kloetzel,Min Jae Lee,Kyung B Kim,Harinder Singh,James J DriscollPubMed 31595669
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human PSMB1. Database link P20618
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
Alternative names
PSC5, PSMB1, Proteasome subunit beta type-1, Macropain subunit C5, Multicatalytic endopeptidase complex subunit C5, Proteasome component C5, Proteasome gamma chain, Proteasome subunit beta-6, beta-6
Recommended products
Rabbit Polyclonal PSB1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PSMB1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human PSMB1. Database link P20618
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PSMB1 also known as proteasome subunit beta type-1 or XAPC7 plays an important role in the proteasome a multicatalytic proteinase complex. The molecular weight of PSMB1 is approximately 26 kDa. PSMB1 expression occurs in various tissues suggesting its wide involvement in cellular processes. This subunit contributes to the proteolytic activity of the 20S core within the proteasome facilitating protein degradation which is essential for maintaining cellular homeostasis.
- Biological function summary
PSMB1 functions as part of the proteasome's 20S core particle. This complex is responsible for most of the protein degradation in cells particularly targeting misfolded or damaged proteins for ubiquitin-mediated proteolysis. The breakdown of proteins is essential for cellular processes like cell cycle regulation and response to oxidative stress. PSMB1 also helps in the processing of antigens for presentation on MHC class I molecules influencing immune surveillance.
- Pathways
PSMB1 involvement is significant in the ubiquitin-proteasome pathway a central pathway in protein catabolism. This pathway enables the cell to control protein levels and quality interacting closely with proteins such as ubiquitin and other proteasome subunits like PSMC2 or PSMA3. Additionally PSMB1 contributes to the regulation of the NF-kB signaling pathway which is important for immune and inflammatory responses by degrading inhibitors that keep NF-kB inactive.
- Associated diseases and disorders
PSMB1 has connections with cancer and neurodegenerative diseases like Alzheimer’s. Its role in protein degradation impacts the accumulation of dysfunctional proteins that characterize these diseases. Overexpression or mutation of PSMB1 may affect its interaction with other proteins like beta-amyloid in Alzheimer's or oncogenes in cancer which can lead to pathological states. Understanding PSMB1's function can aid in developing therapeutic strategies targeting proteostasis-related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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4 product images
Western blot - Anti-PSMB1 antibody (ab196623)
All lanes: Western blot - Anti-PSMB1 antibody (ab196623) at 1/500 dilution
Lane 1: MCF7 cell extract
Lane 2: HL60 cell extract
Lane 3: SW480 cell extract
Lane 4: HepG2 cell extract
Lane 5: SW620 cell extract
Lane 6: K562 cell extract
Lane 7: PC3 cell extract
Lane 8: Mouse skeletal muscle cell extract
Lane 9: Mouse liver cell extract
Developed using the ECL technique.
Predicted band size: 26 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PSMB1 antibody (ab196623)
Immunofluorscence analysis of U20S cells labeling PSMB1 with ab196623 at 1/50 dilution (top panel).
Blue: DAPI for nuclear staining (bottom panel).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB1 antibody (ab196623)
Immunohistochemical analysis of formalin fixed and paraffin embedded rat testis labeling PSMB1 with ab196623 at a 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB1 antibody (ab196623)
Immunohistochemical analysis of formalin fixed and paraffin embedded human breast cancer tissue labeling PSMB1 with ab196623 at a 1/200 dilution.
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