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AB229491

Anti-PSMB1 antibody

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(1 Publication)

Rabbit Polyclonal PSB1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PSMB1.

View Alternative Names

PSC5, PSMB1, Proteasome subunit beta type-1, Macropain subunit C5, Multicatalytic endopeptidase complex subunit C5, Proteasome component C5, Proteasome gamma chain, Proteasome subunit beta-6, beta-6

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB1 antibody (AB229491)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB1 antibody (AB229491)

Paraffin-embedded rat brain tissue stained for PSMB1 with ab229491 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB1 antibody (AB229491)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB1 antibody (AB229491)

Paraffin-embedded mouse brain tissue stained for PSMB1 with ab229491 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-PSMB1 antibody (AB229491)
  • WB

Supplier Data

Western blot - Anti-PSMB1 antibody (AB229491)

12% SDS-PAGE

All lanes:

Western blot - Anti-PSMB1 antibody (ab229491) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Predicted band size: 26 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PSMB1. The exact immunogen used to generate this antibody is proprietary information.

P20618

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Alternative Products

Primary Antibodies

AB196623

Anti-PSMB1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB1 antibody (AB196623)

  • 0
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Complementary Products

Primary Antibodies

AB22673

Anti-Proteasome 20S alpha + beta antibody

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Primary Antibodies

AB150392

Anti-Proteasome 20S beta 6 antibody [EPR9684]

RabMAb|Recombinant

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Primary Antibodies

AB166628

Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (AB166628)

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Primary Antibodies

AB154745

Anti-Proteasome subunit beta type-7 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Proteasome subunit beta type-7 antibody (AB154745)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSMB1 also known as proteasome subunit beta type-1 or XAPC7 plays an important role in the proteasome a multicatalytic proteinase complex. The molecular weight of PSMB1 is approximately 26 kDa. PSMB1 expression occurs in various tissues suggesting its wide involvement in cellular processes. This subunit contributes to the proteolytic activity of the 20S core within the proteasome facilitating protein degradation which is essential for maintaining cellular homeostasis.
Biological function summary

PSMB1 functions as part of the proteasome's 20S core particle. This complex is responsible for most of the protein degradation in cells particularly targeting misfolded or damaged proteins for ubiquitin-mediated proteolysis. The breakdown of proteins is essential for cellular processes like cell cycle regulation and response to oxidative stress. PSMB1 also helps in the processing of antigens for presentation on MHC class I molecules influencing immune surveillance.

Pathways

PSMB1 involvement is significant in the ubiquitin-proteasome pathway a central pathway in protein catabolism. This pathway enables the cell to control protein levels and quality interacting closely with proteins such as ubiquitin and other proteasome subunits like PSMC2 or PSMA3. Additionally PSMB1 contributes to the regulation of the NF-kB signaling pathway which is important for immune and inflammatory responses by degrading inhibitors that keep NF-kB inactive.

PSMB1 has connections with cancer and neurodegenerative diseases like Alzheimer’s. Its role in protein degradation impacts the accumulation of dysfunctional proteins that characterize these diseases. Overexpression or mutation of PSMB1 may affect its interaction with other proteins like beta-amyloid in Alzheimer's or oncogenes in cancer which can lead to pathological states. Understanding PSMB1's function can aid in developing therapeutic strategies targeting proteostasis-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
See full target information PSMB1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 11:692265 PubMed35083134

2022

LINC00883 Promotes Drug Resistance of Glioma Through a microRNA-136/NEK1-Dependent Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Yongzhe Li,Xin Gao
View all publications
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Product promise

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