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AB183506

Anti-PSMB10/MECL1 antibody [EPR14902]

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(16 Publications)

Rabbit Recombinant Monoclonal PSMB10/MECL1 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 16 publications.

View Alternative Names

LMP10, MECL1, PSMB10, Proteasome subunit beta type-10, Low molecular mass protein 10, Macropain subunit MECl-1, Multicatalytic endopeptidase complex subunit MECl-1, Proteasome MECl-1, Proteasome subunit beta-2i

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506)

Immunohistochemical analysis of paraffin-embedded Human Kidney adenocarcinoma tissue labeling PSMB10/MECL1 with ab183506 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506)

Intracellular flow cytometric analysis of Daudi cells (2% paraformaldehyde-fixed) labeling PSMB10/MECL1 with ab183506 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506)
  • WB

Lab

Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506)

Lanes 1-4 : Merged signal (red and green). Green - ab183506 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.

ab183506 Anti-PSMB10/MECL1 antibody [EPR14902] was shown to specifically react with PSMB10/MECL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264923 (knockout cell lysate ab258145) was used. Wild-type and PSMB10/MECL1 knockout samples were subjected to SDS-PAGE. ab183506 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PSMB10 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PSMB10 (MECL1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-psmb10-mecl1-knockout-hela-cell-line-ab264923'>ab264923</a>)

Lane 3:

Daudi cell lysate at 20 µg

Lane 4:

Raji cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506)
  • WB

Supplier Data

Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506)

All lanes:

Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/1000 dilution

Lane 1:

Human fetal spleen tissue lysate at 10 µg

Lane 2:

Human fetal thymus tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 29 kDa

false

Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506)
  • WB

Supplier Data

Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506)

All lanes:

Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/5000 dilution

Lane 1:

HuT 78 cell line lysate at 10 µg

Lane 2:

Raji cell line lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 29 kDa

false

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PSMB10/MECL1 antibody [EPR14902]

  • Carrier free

    Anti-PSMB10/MECL1 antibody [EPR14902] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14902

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/90", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSMB10 also known as MECL1 is a proteasome component involved in protein degradation. This protein has a molecular mass of approximately 29 kDa. PSMB10 is expressed in various tissues with notable expression in the immune system. As a beta subunit of the 20S proteasome MECL1 has a role in cleaving peptides at specific sites contributing to the breakdown of ubiquitinated proteins.
Biological function summary

PSMB10/MECL1 plays a significant role in immune responses. It forms part of the immunoproteasome a variant of the proteasome complex which enhances the generation of antigenic peptides presented on MHC class I molecules. This function is essential for effective immune surveillance and initializing adaptive immunity. In particular PSMB10's influence on antigen processing positions it as an important player in modulating immune system activity.

Pathways

PSMB10/MECL1 participates in pathways associated with protein catabolism and immune signaling. It is involved in the ubiquitin-proteasome pathway which is critical for maintaining cellular protein balance. The function of PSMB10 within the immunoproteasome links it to immune signaling pathways. It has associations with other 20S proteasome subunits such as PSMB8 and PSMB9 which also contribute to the formation of the immunoproteasome complex.

PSMB10/MECL1 has connections to autoimmune diseases and cancer. In autoimmune diseases the altered function of PSMB10 within the immunoproteasome can lead to improper antigen presentation potentially triggering excessive immune responses. In oncology aberrant expression of immunoproteasome subunits including PSMB10 has implications in tumor immune evasion. The interplay with proteins like PSMB9 in these contexts suggests an intricate network affecting disease progression and immune regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.
See full target information PSMB10
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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 44:170 PubMed40462177

2025

PSMB10 maintains the stemness of chemotherapeutic drug-resistant leukemia cells by inhibiting senescence and cytotoxic T lymphocyte-mediated killing in a ubiquitinated degradation manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Ma,Yingnan Li,Di Wang,Jialan Niu,Qian Li,Ying Chen,Mengyuan Wang,Jin Wen,Chenxi Liao,Nan Wang,Xiaolan Zhang,Jiwei Chang,Jiayi Yang,Lei Li,Jing Zou,Danyue Peng,Lingbo Liu

iScience 28:111873 PubMed40060909

2025

Mutational pressure promotes release of public CD8 T cell epitopes by proteasome from SARS-CoV-2 RBD of Omicron and its current lineages.

Applications

Unspecified application

Species

Unspecified reactive species

Anna A Kudriaeva,Ivan O Butenko,George A Saratov,Maxim Т Ri,Yuliana A Mokrushina,Alexey A Bondarev,Alena S Evpak,Ivan V Smirnov,Daria S Matyushkina,Alexander G Gabibov,Vadim M Govorun,Alexey A Belogurov

Proceedings of the National Academy of Sciences of the United States of America 122:e2419607122 PubMed39964708

2025

Tracking proteasome degradation: A cross-organ analysis via intact degradomics mass spectrometry.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina I Zittlau,Daniel Zachor-Movshovitz,Yegor Leushkin,Roy Schimmel Brener,David Morgenstern,Gili Ben-Nissan,Michal Sharon

Cell biology and toxicology 40:82 PubMed39320524

2024

ACE2 alleviates sepsis-induced cardiomyopathy through inhibiting M1 macrophage via NF-κB/STAT1 signals.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Xiao,Jia-Xin Li,Hui-Hua Li,Fei Teng

Scientific reports 12:14087 PubMed35982220

2022

STUB1 is an intracellular checkpoint for interferon gamma sensing.

Applications

Unspecified application

Species

Unspecified reactive species

Simon Ng,Shuhui Lim,Adrian Chong Nyi Sim,Ruban Mangadu,Ally Lau,Chunsheng Zhang,Sarah Bollinger Martinez,Arun Chandramohan,U-Ming Lim,Samantha Shu Wen Ho,Shih Chieh Chang,Pooja Gopal,Lewis Z Hong,Adam Schwaid,Aaron Zefrin Fernandis,Andrey Loboda,Cai Li,Uyen Phan,Brian Henry,Anthony W Partridge

Immune network 22:e28 PubMed35799704

2022

Evaluation of Immunoproteasome-Specific Proteolytic Activity Using Fluorogenic Peptide Substrates.

Applications

Unspecified application

Species

Unspecified reactive species

Sumin Kim,Seo Hyeong Park,Won Hoon Choi,Min Jae Lee

Cell reports. Medicine 2:100455 PubMed35028603

2021

Engineering immunoproteasome-expressing mesenchymal stromal cells: A potent cellular vaccine for lymphoma and melanoma in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jamilah Abusarah,Fatemeh Khodayarian,Nehme El-Hachem,Natasha Salame,Martin Olivier,Mohammad Balood,Katiane Roversi,Sebastien Talbot,Jean-Pierre Bikorimana,Jingkui Chen,Mario Jolicoeur,Louis-Eric Trudeau,Samaneh Kamyabiazar,Borhane Annabi,Francis Robert,Jerry Pelletier,Abed-El-Hakim El-Kadiry,Riam Shammaa,Moutih Rafei

Neuropathology and applied neurobiology 47:1019-1032 PubMed34048071

2021

TDP-43 proteinopathy occurs independently of autophagic substrate accumulation and underlies nuclear defects in Niemann-Pick C disease.

Applications

Unspecified application

Species

Unspecified reactive species

Elaine A Liu,Erika Mori,Fuko Hamasaki,Andrew P Lieberman

Frontiers in immunology 11:603278 PubMed33552061

2021

Co-Inhibition of the Immunoproteasome Subunits LMP2 and LMP7 Ameliorates Immune Thrombocytopenia.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng-Hong Du,Yu-Jiao Xiang,Lu Liu,Mu Nie,Yu Hou,Ling Wang,Ban-Ban Li,Miao Xu,Qing-Liang Teng,Jun Peng,Ming Hou,Yan Shi

Frontiers in cell and developmental biology 8:592048 PubMed33195259

2020

Deficiency of LMP10 Attenuates Diet-Induced Atherosclerosis by Inhibiting Macrophage Polarization and Inflammation in Apolipoprotein E Deficient Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jiawei Liao,Xiangbo An,Xiaolei Yang,Qiu-Yue Lin,Shuang Liu,Yunpeng Xie,Jie Bai,Yun-Long Xia,Hui-Hua Li
View all publications
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