Rabbit Recombinant Monoclonal PSMB10/MECL1 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 12 publications.
Images
![Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506), expandable thumbnail](https://content.abcam.com/products/images/psmb10-mecl1-antibody-epr14902-ab183506--western-blot-img5033.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506), expandable thumbnail](https://content.abcam.com/products/images/psmb10-mecl1-antibody-epr14902-ab183506--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img32215.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506)")
![Flow Cytometry (Intracellular) - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506), expandable thumbnail](https://content.abcam.com/products/images/psmb10-mecl1-antibody-epr14902-ab183506--flow-cytometry-intracellular-img59837.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506)")
![Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506), expandable thumbnail](https://content.abcam.com/products/images/psmb10-mecl1-antibody-epr14902-ab183506--western-blot-img53899.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506)")
![Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506), expandable thumbnail](https://content.abcam.com/products/images/psmb10-mecl1-antibody-epr14902-ab183506--western-blot-img122900.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506)")
Publications
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- Scientific reports 12:140872022STUB1 is an intracellular checkpoint for interferon gamma sensing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimon Ng,Shuhui Lim,Adrian Chong Nyi Sim,Ruban Mangadu,Ally Lau,Chunsheng Zhang,Sarah Bollinger Martinez,Arun Chandramohan,U-Ming Lim,Samantha Shu Wen Ho,Shih Chieh Chang,Pooja Gopal,Lewis Z Hong,Adam Schwaid,Aaron Zefrin Fernandis,Andrey Loboda,Cai Li,Uyen Phan,Brian Henry,Anthony W PartridgePubMed 35982220
- Immune network 22:e282022Evaluation of Immunoproteasome-Specific Proteolytic Activity Using Fluorogenic Peptide Substrates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSumin Kim,Seo Hyeong Park,Won Hoon Choi,Min Jae LeePubMed 35799704
- Cell reports. Medicine 2:1004552021Engineering immunoproteasome-expressing mesenchymal stromal cells: A potent cellular vaccine for lymphoma and melanoma in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJamilah Abusarah,Fatemeh Khodayarian,Nehme El-Hachem,Natasha Salame,Martin Olivier,Mohammad Balood,Katiane Roversi,Sebastien Talbot,Jean-Pierre Bikorimana,Jingkui Chen,Mario Jolicoeur,Louis-Eric Trudeau,Samaneh Kamyabiazar,Borhane Annabi,Francis Robert,Jerry Pelletier,Abed-El-Hakim El-Kadiry,Riam Shammaa,Moutih RafeiPubMed 35028603
- Neuropathology and applied neurobiology 47:1019-10322021TDP-43 proteinopathy occurs independently of autophagic substrate accumulation and underlies nuclear defects in Niemann-Pick C disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElaine A Liu,Erika Mori,Fuko Hamasaki,Andrew P LiebermanPubMed 34048071
- Frontiers in immunology 11:6032782021Co-Inhibition of the Immunoproteasome Subunits LMP2 and LMP7 Ameliorates Immune Thrombocytopenia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSheng-Hong Du,Yu-Jiao Xiang,Lu Liu,Mu Nie,Yu Hou,Ling Wang,Ban-Ban Li,Miao Xu,Qing-Liang Teng,Jun Peng,Ming Hou,Yan ShiPubMed 33552061
- Frontiers in cell and developmental biology 8:5920482020Deficiency of LMP10 Attenuates Diet-Induced Atherosclerosis by Inhibiting Macrophage Polarization and Inflammation in Apolipoprotein E Deficient Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiawei Liao et. al.PubMed 33195259
- American journal of physiology. Endocrinology and 318:E892-E9002020The inducible β5i proteasome subunit contributes to proinsulin degradation in GRP94-deficient β-cells and is overexpressed in type 2 diabetes pancreatic islets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuhammad Saad Khilji et. al.PubMed 32255680
- PloS one 15:e02224322020The intermediate proteasome is constitutively expressed in pancreatic beta cells and upregulated by stimulatory, low concentrations of interleukin 1 β.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuhammad Saad Khilji et. al.PubMed 32053590
- Frontiers in oncology 9:11472019Clinical and Expression Significance of AKT1 by Co-expression Network Analysis in Endometrial Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao Huo et. al.PubMed 31781484
- Virology 537:74-832019Host cell protein PSMB10 interacts with viral NS3 protein and inhibits the growth of classical swine fever virus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaofeng Deng et. al.PubMed 31493657
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/90 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.
Alternative names
LMP10, MECL1, PSMB10, Proteasome subunit beta type-10, Low molecular mass protein 10, Macropain subunit MECl-1, Multicatalytic endopeptidase complex subunit MECl-1, Proteasome MECl-1, Proteasome subunit beta-2i
Recommended products
Rabbit Recombinant Monoclonal PSMB10/MECL1 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 12 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR14902
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PSMB10 also known as MECL1 is a proteasome component involved in protein degradation. This protein has a molecular mass of approximately 29 kDa. PSMB10 is expressed in various tissues with notable expression in the immune system. As a beta subunit of the 20S proteasome MECL1 has a role in cleaving peptides at specific sites contributing to the breakdown of ubiquitinated proteins.
- Biological function summary
PSMB10/MECL1 plays a significant role in immune responses. It forms part of the immunoproteasome a variant of the proteasome complex which enhances the generation of antigenic peptides presented on MHC class I molecules. This function is essential for effective immune surveillance and initializing adaptive immunity. In particular PSMB10's influence on antigen processing positions it as an important player in modulating immune system activity.
- Pathways
PSMB10/MECL1 participates in pathways associated with protein catabolism and immune signaling. It is involved in the ubiquitin-proteasome pathway which is critical for maintaining cellular protein balance. The function of PSMB10 within the immunoproteasome links it to immune signaling pathways. It has associations with other 20S proteasome subunits such as PSMB8 and PSMB9 which also contribute to the formation of the immunoproteasome complex.
- Associated diseases and disorders
PSMB10/MECL1 has connections to autoimmune diseases and cancer. In autoimmune diseases the altered function of PSMB10 within the immunoproteasome can lead to improper antigen presentation potentially triggering excessive immune responses. In oncology aberrant expression of immunoproteasome subunits including PSMB10 has implications in tumor immune evasion. The interplay with proteins like PSMB9 in these contexts suggests an intricate network affecting disease progression and immune regulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506), expandable thumbnail](https://content.abcam.com/products/images/psmb10-mecl1-antibody-epr14902-ab183506--western-blot-img5033.jpg "Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)")
Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
Lanes 1-4: Merged signal (red and green). Green - ab183506 observed at 29 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.ab183506 Anti-PSMB10/MECL1 antibody [EPR14902] was shown to specifically react with PSMB10/MECL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human PSMB10 (MECL1) knockout HeLa cell line ab264923 (knockout cell lysate Human PSMB10 (MECL1) knockout HeLa cell lysate ab258145) was used. Wild-type and PSMB10/MECL1 knockout samples were subjected to SDS-PAGE. ab183506 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: PSMB10 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human PSMB10 (MECL1) knockout HeLa cell line (Human PSMB10 (MECL1) knockout HeLa cell line ab264923)
Lane 3: Daudi cell lysate at 20 µg
Lane 4: Raji cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506), expandable thumbnail](https://content.abcam.com/products/images/psmb10-mecl1-antibody-epr14902-ab183506--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img32215.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
Immunohistochemical analysis of paraffin-embedded Human Kidney adenocarcinoma tissue labeling PSMB10/MECL1 with ab183506 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506), expandable thumbnail](https://content.abcam.com/products/images/psmb10-mecl1-antibody-epr14902-ab183506--flow-cytometry-intracellular-img59837.jpg "Flow Cytometry (Intracellular) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)")
Flow Cytometry (Intracellular) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
Intracellular flow cytometric analysis of Daudi cells (2% paraformaldehyde-fixed) labeling PSMB10/MECL1 with ab183506 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
![Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506), expandable thumbnail](https://content.abcam.com/products/images/psmb10-mecl1-antibody-epr14902-ab183506--western-blot-img53899.jpg "Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)")
Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
All lanes: Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/1000 dilution
Lane 1: Human fetal spleen tissue lysate at 10 µg
Lane 2: Human fetal thymus tissue lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 29 kDa
![Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506), expandable thumbnail](https://content.abcam.com/products/images/psmb10-mecl1-antibody-epr14902-ab183506--western-blot-img122900.jpg "Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)")
Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
All lanes: Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/5000 dilution
Lane 1: HuT 78 cell line lysate at 10 µg
Lane 2: Raji cell line lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 29 kDa
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