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AB190790

Anti-PSMB10/MECL1 antibody [EPR14903]

1

(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal PSMB10/MECL1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

LMP10, MECL1, PSMB10, Proteasome subunit beta type-10, Low molecular mass protein 10, Macropain subunit MECl-1, Multicatalytic endopeptidase complex subunit MECl-1, Proteasome MECl-1, Proteasome subunit beta-2i

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PSMB10/MECL1 antibody [EPR14903] (AB190790)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PSMB10/MECL1 antibody [EPR14903] (AB190790)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PSMB10/MECL1 with ab190790 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor555) secondary antibody at 1/200 dilution. Counter stained with DAPI.

Western blot - Anti-PSMB10/MECL1 antibody [EPR14903] (AB190790)
  • WB

Supplier Data

Western blot - Anti-PSMB10/MECL1 antibody [EPR14903] (AB190790)

All lanes:

Western blot - Anti-PSMB10/MECL1 antibody [EPR14903] (ab190790) at 1/1000 dilution

Lane 1:

Human fetal thymus lysate at 20 µg

Lane 2:

Daudi cell lysate at 20 µg

Lane 3:

Raji cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

  • Carrier free

    Anti-PSMB10/MECL1 antibody [EPR14903] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14903

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB246782

Alexa Fluor® 488 Anti-PSMB10/MECL1 antibody [EPR14902]

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Primary Antibodies

AB240272

Anti-PSMB10/MECL1 antibody [EPR14902] - BSA and Azide free

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] - BSA and Azide free (AB240272)

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  • 0
Primary Antibodies

AB183506

Anti-PSMB10/MECL1 antibody [EPR14902]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506)

  • 0
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Primary Antibodies

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Primary Antibodies

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Anti-Proteasome 20S C2/HC2 antibody [EPR5451]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSMB10 also known as MECL1 is a proteasome component involved in protein degradation. This protein has a molecular mass of approximately 29 kDa. PSMB10 is expressed in various tissues with notable expression in the immune system. As a beta subunit of the 20S proteasome MECL1 has a role in cleaving peptides at specific sites contributing to the breakdown of ubiquitinated proteins.
Biological function summary

PSMB10/MECL1 plays a significant role in immune responses. It forms part of the immunoproteasome a variant of the proteasome complex which enhances the generation of antigenic peptides presented on MHC class I molecules. This function is essential for effective immune surveillance and initializing adaptive immunity. In particular PSMB10's influence on antigen processing positions it as an important player in modulating immune system activity.

Pathways

PSMB10/MECL1 participates in pathways associated with protein catabolism and immune signaling. It is involved in the ubiquitin-proteasome pathway which is critical for maintaining cellular protein balance. The function of PSMB10 within the immunoproteasome links it to immune signaling pathways. It has associations with other 20S proteasome subunits such as PSMB8 and PSMB9 which also contribute to the formation of the immunoproteasome complex.

PSMB10/MECL1 has connections to autoimmune diseases and cancer. In autoimmune diseases the altered function of PSMB10 within the immunoproteasome can lead to improper antigen presentation potentially triggering excessive immune responses. In oncology aberrant expression of immunoproteasome subunits including PSMB10 has implications in tumor immune evasion. The interplay with proteins like PSMB9 in these contexts suggests an intricate network affecting disease progression and immune regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.
See full target information PSMB10
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular & cellular proteomics : MCP 15:3297-3320 PubMed27451424

2016

Proteoform-Specific Insights into Cellular Proteome Regulation.

Applications

WB

Species

Human

Emma L Norris,Madeleine J Headlam,Keyur A Dave,David D Smith,Alexander Bukreyev,Toshna Singh,Buddhika A Jayakody,Keith J Chappell,Peter L Collins,Jeffrey J Gorman
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com