AB137067

Anti-PSMB4 antibody [EPR9396]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal PSMB4 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

PROS26, PSMB4, Proteasome subunit beta type-4, 26 kDa prosomal protein, Macropain beta chain, Multicatalytic endopeptidase complex beta chain, Proteasome beta chain, Proteasome chain 3, Proteasome subunit beta-7, HsBPROS26, PROS-26, HsN3, beta-7

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB4 antibody [EPR9396] (AB137067)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB4 antibody [EPR9396] (AB137067)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling PSMB4 with ab137067 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-PSMB4 antibody [EPR9396] (AB137067)

All lanes:

Western blot - Anti-PSMB4 antibody [EPR9396] (ab137067) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Predicted band size: 29 kDa

false

Carrier free

Anti-PSMB4 antibody [EPR9396] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9396

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSMB4 also known as proteasome subunit beta type-4 is a critical component of the 20S proteasome complex involved in protein degradation. Its molecular mass is approximately 29 kDa. PSMB4 expression occurs widely including in the cytoplasm and nucleus of eukaryotic cells. It plays an important role in regulating protein turnover by participating in the breakdown of ubiquitinated proteins essential for maintaining cellular protein homeostasis.

Biological function summary

The 20S proteasome where PSMB4 belongs acts as the proteolytic core of the 26S proteasome complex. This complex degrades unwanted or damaged proteins thereby regulating various cellular processes such as the cell cycle apoptosis and DNA repair. PSMB4's activity ensures the removal of potentially toxic proteins which is important for cell function and survival.

Pathways

PSMB4 is involved in key pathways such as the ubiquitin-proteasome pathway and antigen processing. These pathways regulate protein metabolism and assist in immune surveillance by presenting endogenous antigens through MHC class I molecules. PSMB4 interacts closely with other proteasome subunits like PSMB5 and PSMB6 which coordinate to cleave peptide bonds in substrate proteins effectively.

Mutations or dysregulation of PSMB4 link to multiple myeloma and neurodegenerative diseases. In multiple myeloma changes in PSMB4 may affect proteasome inhibitor effectiveness while in neurodegenerative disorders impaired protein degradation can lead to protein aggregation. PSMB4 has potential interactions with proteins such as ubiquitin and PSMC3 which further influence disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.

See full target information PSMB4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 53:1092-1108 PubMed34188179

2021

Disruption of nucleocytoplasmic trafficking as a cellular senescence driver.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Hwan Park,Sung Jin Ryu,Byung Ju Kim,Hyun-Ji Cho,Chi Hyun Park,Hyo Jei Claudia Choi,Eun-Jin Jang,Eun Jae Yang,Jeong-A Hwang,Seung-Hwa Woo,Jun Hyung Lee,Ji Hwan Park,Kyung-Mi Choi,Young-Yon Kwon,Cheol-Koo Lee,Joon Tae Park,Sung Chun Cho,Yun-Il Lee,Sung Bae Lee,Jeong A Han,Kyung A Cho,Min-Sik Kim,Daehee Hwang,Young-Sam Lee,Sang Chul Park

Cellular physiology and biochemistry : internation 45:819-831 PubMed29414809

2018

Interference with PSMB4 Expression Exerts an Anti-Tumor Effect by Decreasing the Invasion and Proliferation of Human Glioblastoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Chen Cheng,Wen-Chiuan Tsai,Yu-Chi Sung,Hsin-Han Chang,Ying Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PSMB4 antibody [EPR9396]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB4 antibody [EPR9396] (AB137067)

Western blot - Anti-PSMB4 antibody [EPR9396] (AB137067)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise