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AB137476

Anti-PSMC5 antibody

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(1 Publication)

Rabbit Polyclonal PSMC5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PSMC5 aa 50 to C-terminus.

View Alternative Names

SUG1, PSMC5, 26S proteasome regulatory subunit 8, 26S proteasome AAA-ATPase subunit RPT6, Proteasome 26S subunit ATPase 5, Proteasome subunit p45, Thyroid hormone receptor-interacting protein 1, p45/SUG, TRIP1

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-PSMC5 antibody (AB137476)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PSMC5 antibody (AB137476)

Immunofluorescent analysis of paraformaldehyde-fixed A549 cells labelling PSMC5 with ab137476 at 1/200 dilution. Lower image merged with DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody (AB137476)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody (AB137476)

Immunohistochemical analysis of paraffin embedded Cal27 xenograft labelling PSMC5 with ab137476 at a dilution of 1/500.

Western blot - Anti-PSMC5 antibody (AB137476)
  • WB

Unknown

Western blot - Anti-PSMC5 antibody (AB137476)

10% SDS PAGE

All lanes:

Western blot - Anti-PSMC5 antibody (ab137476) at 1/1000 dilution

Lane 1:

A431 whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Predicted band size: 46 kDa

false

Western blot - Anti-PSMC5 antibody (AB137476)
  • WB

Unknown

Western blot - Anti-PSMC5 antibody (AB137476)

10% SDS PAGE

All lanes:

Western blot - Anti-PSMC5 antibody (ab137476) at 1/1000 dilution

All lanes:

PC12 whole cell lysate at 30 µg

Predicted band size: 46 kDa

false

Western blot - Anti-PSMC5 antibody (AB137476)
  • WB

Unknown

Western blot - Anti-PSMC5 antibody (AB137476)

10% SDS PAGE

All lanes:

Western blot - Anti-PSMC5 antibody (ab137476) at 1/1000 dilution

Lane 1:

Neuro2A whole cell lysate at 30 µg

Lane 2:

GL261 whole cell lysate at 30 µg

Lane 3:

C8D30 whole cell lysate at 30 µg

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human PSMC5 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P62195

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSMC5 also known as Tbp1 or Rpt6 is a component of the 26S proteasome complex that functions in the degradation of ubiquitinated proteins. This protein has a molecular mass of approximately 44 kDa. It shows a widespread pattern of expression being present in various tissues across the human body particularly in tissues with high metabolic activity. As an ATPase associated with diverse cellular activities the main mechanical function of PSMC5 is driving protein unfolding which is essential for proteasome activity.
Biological function summary

PSMC5 is integral to maintaining protein homeostasis and regulating protein turnover within cells. It forms part of the regulatory particle of the proteasome complex engaging in the recognition and unfolding of polyubiquitinated substrates. This action facilitates the degradation process important for numerous cellular processes including cell cycle apoptosis and DNA damage response. The presence of PSMC5 in these pathways indicates its involvement in pivotal cellular functions that regulate cell health and survival.

Pathways

PSMC5 plays a fundamental role in the ubiquitin-proteasome pathway a critical system for protein degradation and turnover. This pathway regulates the concentration of several key regulatory proteins therefore maintaining cellular homeostasis. PSMC5 interacts with other core proteasome components including PSMC1 and PSMC2. Additionally it contributes to the DNA repair pathways by interacting with proteins involved in genotoxic stress response underlining its significance in maintaining genomic stability.

PSMC5 activity has associations with disorders such as cancer and neurodegenerative diseases. Dysregulation of the proteasome function may lead to aberrant protein accumulation which constitutes a factor in the development of these diseases. In cancer PSMC5 can interact with oncogenic pathways such as those involving proteins like MDM2 a ubiquitin ligase influencing tumorigenesis. Similarly in neurodegenerative disorders PSMC5's role in proteostasis connects it to proteins such as ubiquitin impacting disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC5 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.
See full target information PSMC5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 233:6578-6588 PubMed29150931

2018

MicroRNA-497 accelerates apoptosis while inhibiting proliferation, migration, and invasion through negative regulation of the MAPK/ERK signaling pathway via RAF-1.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Tao,Chun-Yu Zhang,Lei Guo,Xia Li,Na-Na Han,Qi Zhou,Zhi-Le Liu
View all publications
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Product promise

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