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AB178681

Anti-PSMC5 antibody [EPR13565(B)]

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(10 Publications)

Rabbit Recombinant Monoclonal PSMC5 antibody. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 10 publications.

View Alternative Names

SUG1, PSMC5, 26S proteasome regulatory subunit 8, 26S proteasome AAA-ATPase subunit RPT6, Proteasome 26S subunit ATPase 5, Proteasome subunit p45, Thyroid hormone receptor-interacting protein 1, p45/SUG, TRIP1

12 Images
Immunocytochemistry/ Immunofluorescence - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)

Immunofluorescent analysis of HeLa cells labeling PSMC5 with unpurified ab178681 at 1/100 dilution.

Flow Cytometry (Intracellular) - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PSMC5 with purified ab178681 at 1/90 dilution (10μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)

Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling PSMC5 with unpurified ab178681 at 1/50 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PSMC5 with Purifiedab178681 at 1/500 dilution (1.7 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling PSMC5 with Purified ab178681 at 1/2000 dilution (0.43 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Flow Cytometry (Intracellular) - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)

Intracellular flow cytometric analysis of permeabilized Hela cellslabeling PSMC5 with unpurified ab178681 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

Immunoprecipitation - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)
  • IP

Unknown

Immunoprecipitation - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)

ab178681 (Purified) at 1/40 dilution (2μg) immunoprecipitating PSMC5 in HepG2 whole cell lysate.
Lane 1 (input) : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate (10μg)
Lane 2 (+) : ab178681 & HepG2 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab178681 in HepG2 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)

Predicted band size: 46 kDa

false

Immunoprecipitation - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)
  • IP

Supplier Data

Immunoprecipitation - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)

Western blot analysis on immunoprecipitation pellet from (Lane 1) 293T cell lysate or (Lane 2) 1XPBS (negative control) using unpurified ab178681 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)

Predicted band size: 44 kDa,46 kDa

Observed band size: 75 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling PSMC5 with Purified ab178681 at 1/2000 dilution (0.43 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling PSMC5 with Purified ab178681 at 1/2000 dilution (0.43 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Western blot - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)
  • WB

Supplier Data

Western blot - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)

All lanes:

Western blot - Anti-PSMC5 antibody [EPR13565(B)] (ab178681) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Predicted band size: 46 kDa

false

Western blot - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)
  • WB

Lab

Western blot - Anti-PSMC5 antibody [EPR13565(B)] (AB178681)

All lanes:

Western blot - Anti-PSMC5 antibody [EPR13565(B)] (ab178681) at 1/1000 dilution

Lane 1:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 3:

Mouse heart lysates at 20 µg

Lane 4:

Mouse brain lysates at 20 µg

Lane 5:

Rat brain lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 46 kDa

Observed band size: 46 kDa

false

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-PSMC5 antibody [EPR13565(B)]

  • Carrier free

    Anti-PSMC5 antibody [EPR13565(B)] - BSA and Azide free

  • 578 PE

    PE Anti-PSMC5 antibody [EPR13565(B)]

  • 660 APC

    APC Anti-PSMC5 antibody [EPR13565(B)]

  • HRP

    HRP Anti-PSMC5 antibody [EPR13565(B)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-PSMC5 antibody [EPR13565(B)]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-PSMC5 antibody [EPR13565(B)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-PSMC5 antibody [EPR13565(B)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PSMC5 antibody [EPR13565(B)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PSMC5 antibody [EPR13565(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13565(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, Flow Cyt (Intra), ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/10000 - 1/50000.</strong></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p><strong>For unpurified use at 1/50 - 1/100</strong><strong>.</strong></p><p>Perform heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/90 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p><strong>For unpurified use at 1/50 - 1/100.</strong></p><p>Perform heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p><strong>For unpurified use at 1/50 - 1/100.</strong></p><p>Perform heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSMC5 also known as Tbp1 or Rpt6 is a component of the 26S proteasome complex that functions in the degradation of ubiquitinated proteins. This protein has a molecular mass of approximately 44 kDa. It shows a widespread pattern of expression being present in various tissues across the human body particularly in tissues with high metabolic activity. As an ATPase associated with diverse cellular activities the main mechanical function of PSMC5 is driving protein unfolding which is essential for proteasome activity.
Biological function summary

PSMC5 is integral to maintaining protein homeostasis and regulating protein turnover within cells. It forms part of the regulatory particle of the proteasome complex engaging in the recognition and unfolding of polyubiquitinated substrates. This action facilitates the degradation process important for numerous cellular processes including cell cycle apoptosis and DNA damage response. The presence of PSMC5 in these pathways indicates its involvement in pivotal cellular functions that regulate cell health and survival.

Pathways

PSMC5 plays a fundamental role in the ubiquitin-proteasome pathway a critical system for protein degradation and turnover. This pathway regulates the concentration of several key regulatory proteins therefore maintaining cellular homeostasis. PSMC5 interacts with other core proteasome components including PSMC1 and PSMC2. Additionally it contributes to the DNA repair pathways by interacting with proteins involved in genotoxic stress response underlining its significance in maintaining genomic stability.

PSMC5 activity has associations with disorders such as cancer and neurodegenerative diseases. Dysregulation of the proteasome function may lead to aberrant protein accumulation which constitutes a factor in the development of these diseases. In cancer PSMC5 can interact with oncogenic pathways such as those involving proteins like MDM2 a ubiquitin ligase influencing tumorigenesis. Similarly in neurodegenerative disorders PSMC5's role in proteostasis connects it to proteins such as ubiquitin impacting disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC5 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.
See full target information PSMC5
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Human molecular genetics 33:1506-1523 PubMed38776958

2024

PSMC5 insufficiency and P320R mutation impair proteasome function.

Applications

Unspecified application

Species

Unspecified reactive species

Zhong-Qiu Yu,Jenny Carmichael,Galen A Collins,Maria Daniela D'Agostino,Mathieu Lessard,Helen V Firth,Pooja Harijan,Andrew E Fry,John Dean,Jiuchun Zhang,Usha Kini,Alfred L Goldberg,David C Rubinsztein

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed38124005

2023

Phosphorylation of RPT6 Controls Its Ability to Bind DNA and Regulate Gene Expression in the Hippocampus of Male Rats during Memory Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Kayla Farrell,Aubrey Auerbach,Madeline Musaus,Shaghayegh Navabpour,Catherine Liu,Yu Lin,Hehuang Xie,Timothy J Jarome

Learning & memory (Cold Spring Harbor, N.Y.) 29:256-264 PubMed36206393

2022

The epigenetic role of proteasome subunit RPT6 during memory formation in female rats.

Applications

Unspecified application

Species

Unspecified reactive species

Kayla Farrell,Aubrey Auerbach,Madeline Musaus,Timothy J Jarome

Traffic (Copenhagen, Denmark) 23:287-304 PubMed35466519

2022

Extracellular 20S proteasome secreted via microvesicles can degrade poorly folded proteins and inhibit Galectin-3 agglutination activity.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Bonhoure,Laurent Henry,Claudia Bich,Lionel Blanc,Blanche Bergeret,Marie-Pierre Bousquet,Olivier Coux,Pierre-Emmanuel Stoebner,Michel Vidal

Nutritional neuroscience 26:290-302 PubMed35282800

2022

Short-term exposure to an obesogenic diet causes dynamic dysregulation of proteasome-mediated protein degradation in the hypothalamus of female rats.

Applications

Unspecified application

Species

Unspecified reactive species

Taylor McFadden,Kayla Farrell,Kiley Martin,Madeline Musaus,Timothy J Jarome

Frontiers in aging neuroscience 13:656944 PubMed33897408

2021

Age-Related Memory Impairment and Sex-Specific Alterations in Phosphorylation of the Rpt6 Proteasome Subunit and Polyubiquitination in the Basolateral Amygdala and Medial Prefrontal Cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Brooke N Dulka,Sydney Trask,Fred J Helmstetter

Biological psychiatry 89:1176-1187 PubMed33934885

2021

Ubiquitination of Histone H2B by Proteasome Subunit RPT6 Controls Histone Methylation Chromatin Dynamics During Memory Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy J Jarome,Gabriella A Perez,William M Webb,Katrina M Hatch,Shaghayegh Navabpour,Madeline Musaus,Kayla Farrell,Rebecca M Hauser,Taylor McFadden,Kiley Martin,Anderson A Butler,Jing Wang,Farah D Lubin

Theranostics 10:2522-2537 PubMed32194817

2020

Therapeutic ultrasound combined with microbubbles improves atherosclerotic plaque stability by selectively destroying the intraplaque neovasculature.

Applications

Unspecified application

Species

Unspecified reactive species

Xinzhong Li,Shengcun Guo,Tong Xu,Xiang He,Yili Sun,Xiaoqiang Chen,Shiping Cao,Xiaoyun Si,Wangjun Liao,Yulin Liao,Yuan Han,Jianping Bin

EBioMedicine 39:69-82 PubMed30545799

2018

Inhibition of AZIN2-sv induces neovascularization and improves prognosis after myocardial infarction by blocking ubiquitin-dependent talin1 degradation and activating the Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinzhong Li,Yili Sun,Senlin Huang,Yanmei Chen,Xiaoqiang Chen,Mengsha Li,Xiaoyun Si,Xiang He,Hao Zheng,Lintao Zhong,Yang Yang,Wangjun Liao,Yulin Liao,Guojun Chen,Jianping Bin

PloS one 10:e0116270 PubMed25643152

2015

Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ∆133p53α mimic.

Applications

WB

Species

Mouse

Sonal Sawhney,Kylie Hood,Alisha Shaw,Antony W Braithwaite,Richard Stubbs,Noelyn A Hung,Janice A Royds,Tania L Slatter
View all publications
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