Anti-PSMC5 antibody [EPR13565(B)] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13565(B)] - BSA and Azide free (AB240208)

Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling PSMC5 with unpurified ab178681 at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178681).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13565(B)] - BSA and Azide free (AB240208)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling PSMC5 with Purified ab178681 at 1/2000 dilution (0.43 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178681)

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PSMC5 antibody [EPR13565(B)] - BSA and Azide free (AB240208)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PSMC5 with Purifiedab178681 at 1/500 dilution (1.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (anti psmc5 antibody epr13565 b immunocytochemistry hela human)

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PSMC5 antibody [EPR13565(B)] - BSA and Azide free (AB240208)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PSMC5 with purified ab178681 at 1/90 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178681)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13565(B)] - BSA and Azide free (AB240208)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling PSMC5 with Purified ab178681 at 1/2000 dilution (0.43 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178681)

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PSMC5 antibody [EPR13565(B)] - BSA and Azide free (AB240208)

Intracellular flow cytometric analysis of permeabilized Hela cellslabeling PSMC5 withunpurified ab178681 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178681).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PSMC5 antibody [EPR13565(B)] - BSA and Azide free (AB240208)

Immunofluorescent analysis of HeLa cells labeling PSMC5 with unpurified ab178681 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178681).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13565(B)] - BSA and Azide free (AB240208)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling PSMC5 with Purified ab178681 at 1/2000 dilution (0.43 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178681)

• IP

Supplier Data

Immunoprecipitation - Anti-PSMC5 antibody [EPR13565(B)] - BSA and Azide free (AB240208)

Western blot analysis on immunoprecipitation pellet from (Lane 1) 293T cell lysate or (Lane 2) 1XPBS (negative control) using unpurified ab178681 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178681).

All lanes:

Immunoprecipitation - Anti-PSMC5 antibody [EPR13565(B)] (<a href='/en-us/products/primary-antibodies/psmc5-antibody-epr13565b-ab178681'>ab178681</a>)

Predicted band size: 44 kDa,46 kDa

Observed band size: 75 kDa

false

• IP

Unknown

Immunoprecipitation - Anti-PSMC5 antibody [EPR13565(B)] - BSA and Azide free (AB240208)

ab178681 (Purified) at 1 : 40 dilution (2µg) immunoprecipitating PSMC5 in HepG2 whole cell lysate.
Lane 1 (input) : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate (10µg)
Lane 2 (+) : ab178681 & HepG2 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab178681 in HepG2 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (anti psmc5 antibody epr13565 b immunoprecipitation hepg2 human)

All lanes:

Immunoprecipitation - Anti-PSMC5 antibody [EPR13565(B)] (<a href='/en-us/products/primary-antibodies/psmc5-antibody-epr13565b-ab178681'>ab178681</a>)

Predicted band size: 46 kDa

false