Anti-PSMC6 antibody [p42-23]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC6 antibody [p42-23] (AB22639)

IHC image of ab22639 staining in human normal pancrease formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22639, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• Flow Cyt

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Flow Cytometry - Anti-PSMC6 antibody [p42-23] (AB22639)

Overlay histogram showing HeLa cells stained with ab22639 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22639, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

• WB

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Western blot - Anti-PSMC6 antibody [p42-23] (AB22639)

All lanes:

Western blot - Anti-PSMC6 antibody [p42-23] (ab22639) at 1/5000 dilution

Lane 1:

Rpt4 in HeLa S3 cytosolic preparation

Lane 2:

Purified protein

Lane 3:

Proteasome in human placenta

Predicted band size: 44 kDa

Observed band size: 44 kDa

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Exposure time: 1min