Anti-PSMD1 antibody

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-PSMD1 antibody (AB140682)

Immunohistochemical analysis of frozen section of Human breast carcinoma tissue with ab140682 at 1 : 5,000 (0.2μg/ml) dilution.

Detection : DAB

• IP

Unknown

Immunoprecipitation - Anti-PSMD1 antibody (AB140682)

ab140682 at 1 µg/ml detecting PSMD1 in 293T whole cell lysate by WB following IP.

Lane 1 : IP with an antibody which recognizes a different epitope of PSMD1.
Lane 2 : ab140682 at 6 µg/mg of lysate.
Lane 3 : Control IgG.

In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

Detection : Chemiluminescence with an exposure time of 3 seconds

All lanes:

Immunoprecipitation - Anti-PSMD1 antibody (ab140682)

Predicted band size: 105 kDa

false

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-PSMD1 antibody (AB140682)

Immunohistochemical analysis of frozen section of Mouse renal cell carcinoma tissue with ab140682 at 1 : 5,000 (0.2μg/ml) dilution.

Detection : DAB

• WB

Unknown

Western blot - Anti-PSMD1 antibody (AB140682)

All lanes:

Western blot - Anti-PSMD1 antibody (ab140682) at 0.1 µg/mL

Lane 1:

Whole cell lysate prepared from 293T cells at 50 µg

Lane 2:

Whole cell lysate prepared from 293T cells at 15 µg

Lane 3:

Whole cell lysate prepared from HeLa cells at 50 µg

Lane 4:

Whole cell lysate prepared from Jurkat cells at 50 µg

Predicted band size: 105 kDa

true

Exposure time: 10s

• WB

Unknown

Western blot - Anti-PSMD1 antibody (AB140682)

All lanes:

Western blot - Anti-PSMD1 antibody (ab140682) at 0.1 µg/mL

All lanes:

whole cell lysate prepared from NIH3T3 cells at 50 µg

Predicted band size: 105 kDa

true

Exposure time: 30s

• WB

CiteAb

Western blot - Anti-PSMD1 antibody (AB140682)

Western Blotting using Anti-PSMD1 antibody, ab140682. Publication image from Lobanova, E. S. et al., 2018, Nat Commun, 29712894. Legend direct from paper.

Proteasome composition of the mouse retina. a The molar ratio among 20S, 19S, and 11S proteasomal components determined by quantitative mass spectrometry. Data are shown as mean ± SEM; n = 3. b Fractionation of proteasome components in retinal extracts from 1-month-old mice (200 µg total protein) by size-exclusion chromatography on a Superose-6 column. Proteins in 0.5 ml fractions were probed by western blotting using antibodies against β1 subunit of the 20S proteasome core, PSMD11 subunit of the 19S proteasome cap, and PA28α subunit of the 11S cap. Data are taken from one of the four similar experiments. c The distribution of β1, PSMD11, and PA28α in 20 µm serial tangential sections throughout the entire WT mouse retina. Each section was solubilized in 30 µl SDS-PAGE sample buffer for analysis. Proteins were visualized by western blotting using the ECL technique. Rhodopsin was used as a photoreceptor outer segment marker; phosducin was used as a marker of the entire photoreceptor layer. Data are taken from one of two similar experiments. A representative retinal cross-section is shown below western blot panes; the corresponding position of the photoreceptor cells is illustrated by a cartoon

false

• WB

CiteAb

Western blot - Anti-PSMD1 antibody (AB140682)

Western Blotting using Anti-PSMD1 antibody, ab140682. Publication image from Lobanova, E. S. et al., 2018, Nat Commun, 29712894. Legend direct from paper.

Characterization of PA28α and PSMD11 overexpressing (OE) mice. a Western blots of proteasomal subunits in retinal lysates containing 30 µg total protein. Bands were visualized using the LiCor Odyssey imaging system. Each protein was analyzed in at least 3 pairs of 1-month-old WT and overexpressing animals. b Retinal morphology of 3-month-old overexpressing and WT mice. Retinas were embedded in plastic, 1 µm cross-sections were stained by toluidine blue and analyzed by light microscopy. Data are taken from one of the five similar experiments; scale bar : 20 µm. c Chymotrypsin-like proteasomal activity in retinal extracts from 1-month-old overexpressing and WT mice; measurements were performed in the presence or absence of ATP, as indicated. The number of measurements was 10, 7, and 5 for WT, PA28α overexpressing, and PSMD11 overexpressing mice, respectively. The data are shown as mean ± SEM; p values determined across individual preparations are indicated in the text. d Fractionation of proteasomal components in retinal extracts from 2-month-old overexpressing and WT mice by size-exclusion chromatography on a Superose-6 Increase column. Proteins in 0.5 ml fractions were probed by western blotting using antibodies against the β1 subunit of the 20S proteasome core, PSMD11 subunit of the 19S proteasome cap, and PA28α subunit of the11S cap. Data are taken from one of the three similar experiments

false

• WB

CiteAb

Western blot - Anti-PSMD1 antibody (AB140682)

Western Blotting using Anti-PSMD1 antibody, ab140682. Publication image from Lobanova, E. S. et al., 2018, Nat Commun, 29712894. Legend direct from paper.

Overexpression of PA28α or PSMD11 does not affect accumulation of the UbG76V-GFP reporter. The UbG76V-GFP reporter was detected in retinal lysates from 1-month-old mice of indicated genotypes (30 µg total protein/lane) using an anti-GFP antibody; Hsc-70 was used as a loading control. The band representing the non-proteolyzed non-fluorescent GFP product co-accumulating with this reporter in cells suffering from proteasomal insufficiency10,58 is labeled as xGFP. Data are taken from one of the four similar experiments

false

• WB

CiteAb

Western blot - Anti-PSMD1 antibody (AB140682)

Western Blotting using Anti-PSMD1 antibody, ab140682. Publication image from Lobanova, E. S. et al., 2018, Nat Commun, 29712894. Legend direct from paper.

Proteasome composition of the mouse retina. a The molar ratio among 20S, 19S, and 11S proteasomal components determined by quantitative mass spectrometry. Data are shown as mean ± SEM; n = 3. b Fractionation of proteasome components in retinal extracts from 1-month-old mice (200 µg total protein) by size-exclusion chromatography on a Superose-6 column. Proteins in 0.5 ml fractions were probed by western blotting using antibodies against β1 subunit of the 20S proteasome core, PSMD11 subunit of the 19S proteasome cap, and PA28α subunit of the 11S cap. Data are taken from one of the four similar experiments. c The distribution of β1, PSMD11, and PA28α in 20 µm serial tangential sections throughout the entire WT mouse retina. Each section was solubilized in 30 µl SDS-PAGE sample buffer for analysis. Proteins were visualized by western blotting using the ECL technique. Rhodopsin was used as a photoreceptor outer segment marker; phosducin was used as a marker of the entire photoreceptor layer. Data are taken from one of two similar experiments. A representative retinal cross-section is shown below western blot panes; the corresponding position of the photoreceptor cells is illustrated by a cartoon

false

• WB

CiteAb

Western blot - Anti-PSMD1 antibody (AB140682)

Western Blotting using Anti-PSMD1 antibody, ab140682. Publication image from Lobanova, E. S. et al., 2018, Nat Commun, 29712894. Legend direct from paper.

Characterization of PA28α and PSMD11 overexpressing (OE) mice. a Western blots of proteasomal subunits in retinal lysates containing 30 µg total protein. Bands were visualized using the LiCor Odyssey imaging system. Each protein was analyzed in at least 3 pairs of 1-month-old WT and overexpressing animals. b Retinal morphology of 3-month-old overexpressing and WT mice. Retinas were embedded in plastic, 1 µm cross-sections were stained by toluidine blue and analyzed by light microscopy. Data are taken from one of the five similar experiments; scale bar : 20 µm. c Chymotrypsin-like proteasomal activity in retinal extracts from 1-month-old overexpressing and WT mice; measurements were performed in the presence or absence of ATP, as indicated. The number of measurements was 10, 7, and 5 for WT, PA28α overexpressing, and PSMD11 overexpressing mice, respectively. The data are shown as mean ± SEM; p values determined across individual preparations are indicated in the text. d Fractionation of proteasomal components in retinal extracts from 2-month-old overexpressing and WT mice by size-exclusion chromatography on a Superose-6 Increase column. Proteins in 0.5 ml fractions were probed by western blotting using antibodies against the β1 subunit of the 20S proteasome core, PSMD11 subunit of the 19S proteasome cap, and PA28α subunit of the11S cap. Data are taken from one of the three similar experiments

false