AB99413

Anti-PSMD11 antibody

Name: Anti-PSMD11 antibody (ab99413) | Abcam
Brand: Abcam Limited
SKU: AB99413
Price: 535 USD
Availability: InStock
Rating: 4 (5 reviews)

(5 Reviews)

(3 Publications)

Rabbit Polyclonal PSMD11 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PSMD11 aa 150-250.

View Alternative Names

26S proteasome non-ATPase regulatory subunit 11, 26S proteasome regulatory subunit RPN6, 26S proteasome regulatory subunit S9, 26S proteasome regulatory subunit p44.5, PSMD11

2 Images

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Immunoprecipitation - Anti-PSMD11 antibody (AB99413)

Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99413 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane Western Blot : anti-PSMD11 antibody (ab99413) at 0.4 µg/ml

developed using the ECL technique

Exposure time : 10 seconds

Predicted band size : 47 kDa

All lanes:

Immunoprecipitation - Anti-PSMD11 antibody (ab99413)

Predicted band size: 47 kDa

false

Unknown

Western blot - Anti-PSMD11 antibody (AB99413)

All lanes:

Western blot - Anti-PSMD11 antibody (ab99413) at 0.04 µg/mL

Lane 1:

Hela whole cell lysate at 50 µg

Lane 2:

Hela whole cell lysate at 15 µg

Lane 3:

Hela whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 47 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human PSMD11 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

O00231

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSMD11 also known as RPN-6 is a regulatory subunit of the proteasome 26S. It is approximately 50 kDa in mass and plays an essential role in the proteasome's function responsible for protein degradation within cells. PSMD11 is broadly expressed in various tissues including high expression in the brain heart and skeletal muscle. Its mechanical role involves binding to other subunits to stabilize the proteasome structure and facilitate substrate entry into the proteolytic core.

Biological function summary

PSMD11 is essential for the proper assembly and activity of the 26S proteasome complex which is important for ubiquitin-mediated proteolysis. This process controls protein turnover and regulates the concentration of various proteins to maintain cellular homeostasis. PSMD11 enhances the degradation of misfolded or damaged proteins therefore preventing the accumulation of toxic protein aggregates within cells. Its expression levels can modulate the proteasome activity in response to cellular conditions.

Pathways

PSMD11 plays a significant role in the ubiquitin-proteasome pathway which is critical for protein homeostasis and degradation of regulatory proteins. This pathway involves several important proteins including ubiquitin which tags substrates for degradation. PSMD11 also interfaces with signaling pathways that regulate cell cycle progression and apoptosis such as interactions with p53 and c-Myc proteins. These pathways ensure the balance between cell survival and programmed cell death.

PSMD11 links directly to conditions involving proteasome dysfunction such as neurodegenerative diseases including Parkinson's disease and Alzheimer's disease. Dysregulation of PSMD11 can impact proteasome activity leading to the buildup of unwanted proteins that contribute to these conditions. Additionally PSMD11's interaction with proteins like alpha-synuclein and tau in these diseases highlights its relevance in understanding their pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs) : its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.

See full target information PSMD11

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eadm9518 PubMed38478612

2024

Growth factor-induced activation of MSK2 leads to phosphorylation of H3K9me2S10 and corresponding changes in gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Karen G Wong,Yu-Chia F Cheng,Vincent H Wu,Anna A Kiseleva,Jun Li,Andrey Poleshko,Cheryl L Smith,Jonathan A Epstein

Nature aging 3:546-566 PubMed37118550

2023

Cold temperature extends longevity and prevents disease-related protein aggregation through PA28γ-induced proteasomes.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun Ju Lee,Hafiza Alirzayeva,Seda Koyuncu,Amirabbas Rueber,Alireza Noormohammadi,David Vilchez

Communications biology 3:262 PubMed32451438

2020

The ubiquitin-conjugating enzyme UBE2K determines neurogenic potential through histone H3 in human embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Azra Fatima,Dilber Irmak,Alireza Noormohammadi,Markus M Rinschen,Aniruddha Das,Orsolya Leidecker,Christina Schindler,Víctor Sánchez-Gaya,Prerana Wagle,Wojciech Pokrzywa,Thorsten Hoppe,Alvaro Rada-Iglesias,David Vilchez

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PSMD11 antibody

Immunoprecipitation - Anti-PSMD11 antibody (AB99413)

Western blot - Anti-PSMD11 antibody (AB99413)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise