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AB246940

Anti-PSMD12 antibody

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(2 Publications)

Rabbit Polyclonal PSMD12 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PSMD12 aa 250 to C-terminus.

View Alternative Names

26S proteasome non-ATPase regulatory subunit 12, 26S proteasome regulatory subunit RPN5, 26S proteasome regulatory subunit p55, PSMD12

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD12 antibody (AB246940)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD12 antibody (AB246940)

Paraffin-embedded human testis tissue stained for PSMD12 ab246940 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-PSMD12 antibody (AB246940)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PSMD12 antibody (AB246940)

PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling PSMD12 using ab246940 at 4 μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD12 antibody (AB246940)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD12 antibody (AB246940)

Paraffin-embedded human salivary gland tissue stained for PSMD12 using ab246940 at 1/200 dilution in immunohistochemical analysis. Low expression as expected.

Western blot - Anti-PSMD12 antibody (AB246940)
  • WB

Unknown

Western blot - Anti-PSMD12 antibody (AB246940)

All lanes:

Western blot - Anti-PSMD12 antibody (ab246940) at 0.4 µg/mL

Lane 1:

RT4 (Human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (Human brain glioma cell line) cell lysate

Lane 3:

Human plasma (IgG/HSA depleted)

Lane 4:

Human liver tissue lysate

Lane 5:

Human tonsil tissue lysate

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PSMD12 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O00232

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSMD12 also known as proteasome 26S subunit non-ATPase 12 is a component of the 19S proteasome regulatory particle which is involved in the degradation of ubiquitinated proteins. The protein has a calculated mass of approximately 39 kDa. Researchers have found PSMD12 in a wide range of tissues indicating its broad expression across different cell types. It plays a significant role in the ubiquitin-proteasome system influencing several cellular processes.
Biological function summary

PSMD12 functions as an integral part of the proteasome complex. The proteasome which includes PSMD12 plays a critical role in the regulated degradation of damaged or unnecessary proteins thereby maintaining protein homeostasis within cells. This process is essential for regulating various cellular functions such as cell cycle apoptosis and DNA repair. Through its involvement in protein degradation PSMD12 indirectly influences transcriptional regulation and signal transduction.

Pathways

PSMD12 synthesis participates actively in the ubiquitin-proteasome pathway and is associated with cell cycle regulation. The protein's involvement in these pathways underlines its important interaction with other proteasome subunits like PSMD1 and PSMD2. Additionally PSMD12's contribution to the NF-kB signaling pathway suggests its influence on genes responsible for immune and inflammatory responses.

PSMD12 takes part in processes relevant to cancer development and neurodegenerative diseases such as Alzheimer's disease. Altered regulation of the proteasome function involving PSMD12 and related proteins like PSMD7 has been implicated in tumorigenesis where protein degradation pathways become disrupted. Furthermore in Alzheimer’s impaired proteasome activity including the function of components like PSMD12 has been linked to the accumulation of protein aggregates commonly observed in these neurodegenerative disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.
See full target information PSMD12
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & therapy 23:1-11 PubMed36137220

2022

PSMD12 promotes the activation of the MEK-ERK pathway by upregulating KIF15 to promote the malignant progression of liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hanpu Zhang,Chenyuan Li,Shichong Liao,Yi Tu,Shengrong Sun,Feng Yao,Zhiyu Li,Zhong Wang

Histology and histopathology 36:183-194 PubMed33438176

2021

Aldehyde dehydrogenase 1B1 is a potential marker of colorectal tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Hejing Wang,Yanmeng Li,Donghu Zhou,Xiaojin Li,Siyu Jia,Saiping Qi,Jian Huang
View all publications
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Product promise

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