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AB247774

Anti-PSMD14 antibody [EPR4258] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal PSMD14 antibody. Carrier free. Suitable for WB, IP and reacts with Human samples. Cited in 1 publication.

View Alternative Names

POH1, PSMD14, Ubiquitin C-terminal hydrolase PSMD14, 26S proteasome non-ATPase regulatory subunit 14, 26S proteasome regulatory subunit RPN11, 26S proteasome-associated PAD1 homolog 1

2 Images
Immunoprecipitation - Anti-PSMD14 antibody [EPR4258] - BSA and Azide free (AB247774)
  • IP

Lab

Immunoprecipitation - Anti-PSMD14 antibody [EPR4258] - BSA and Azide free (AB247774)

This data was developed using ab109130, the same antibody clone in a different buffer formulation. PSMD14 was immunoprecipitated from 0.35 mg K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 μg with 109130 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 μg

Lane 2 : ab109130 IP in K-562 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab109130 in K-562 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-PSMD14 antibody [EPR4258] (<a href='/en-us/products/primary-antibodies/psmd14-antibody-epr4258-ab109130'>ab109130</a>)

Predicted band size: 35 kDa

Observed band size: 35 kDa

false

Western blot - Anti-PSMD14 antibody [EPR4258] - BSA and Azide free (AB247774)
  • WB

Unknown

Western blot - Anti-PSMD14 antibody [EPR4258] - BSA and Azide free (AB247774)

This data was developed using ab109130, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PSMD14 antibody [EPR4258] (<a href='/en-us/products/primary-antibodies/psmd14-antibody-epr4258-ab109130'>ab109130</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

Namalwa cell lysate at 10 µg

Lane 5:

HeLa cell lysate at 10 µg

Lane 6:

MCF7 cell lysate at 10 µg

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4258

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

ab247774 is the carrier-free version of ab109130.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSMD14 also known as POH1 or RPN11 is a proteasome subunit involved in the regulation of protein degradation. This protein has a molecular mass of approximately 34 kDa and forms an essential part of the 19S regulatory particle of the proteasome complex. PSMD14 is expressed in various tissues but shows significant expression levels in the brain heart and skeletal muscles. By deubiquitinating substrates PSMD14 contributes to the recycling of ubiquitin for further use in the cell’s ubiquitin-proteasome pathway.
Biological function summary

PSMD14 functions as a vital component of the 19S proteasome which is integrated into the proteolytic 26S proteasome complex. The protein's deubiquitinating activity plays a role in maintaining proteostasis by removing ubiquitin chains from substrates destined for degradation. This process is important for regulating protein quality control cell cycle progression and response to oxidative stress among other cellular processes. As part of the proteasome complex PSMD14’s activity impacts cellular homeostasis and contributes to cellular responses to various stimuli.

Pathways

PSMD14 is instrumental in the ubiquitin-proteasome system which is essential for targeted protein degradation modulation of the cell cycle and regulation of transcription factors. It interacts with other proteasome components like PSMD12 and PSMD3 to form a functional proteasome unit. This protein is significant in the NF-kB signaling pathway where it influences the degradation of inhibitor proteins allowing for the activation of NF-kB a critical transcription factor in immune response and cell survival.

PSMD14’s function connects it to cancer and neurodegenerative conditions. Deregulation or mutations in PSMD14 have been implicated in various cancers possibly through disturbed protein degradation which leads to aberrant cell cycle progression and survival. It also relates to neurodegenerative diseases like Alzheimer's as impaired proteasome function can lead to the accumulation of misfolded proteins. PSMD14 interacts with proteins like UCH37 which is also linked to the pathogenesis of these diseases highlighting the significance of controlled proteolysis in maintaining cellular health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair (PubMed : 9374539, PubMed : 1317798). The PSMD14 subunit is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains within the complex (PubMed : 22909820). Plays a role in response to double-strand breaks (DSBs) : acts as a regulator of non-homologous end joining (NHEJ) by cleaving 'Lys-63'-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation (PubMed : 22909820). Also involved in homologous recombination repair by promoting RAD51 loading (PubMed : 22909820). Regulates macroautophagy by ensuring Golgi-to-ER retrograde transport through its deubiquitinating activity on K63-linked ubiquitin chains. This activity prevents the retention of essential autophagy proteins at the Golgi, enabling their trafficking to autophagosome formation sites and supporting Golgi-ER membrane recycling critical for effective autophagy (PubMed : 32210007).
See full target information PSMD14
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:409 PubMed37600337

2023

Renal epithelioid angiomyolipoma: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyun Li,Xuebei Zhang,Qingli Zhao,Jin Wang,Xiaoqing Yang,Shengliang Huang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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