AB226371

Anti-PSMD5 antibody

Name: Anti-PSMD5 antibody (ab226371) | Abcam
Brand: Abcam Limited
SKU: AB226371

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(1 Publication)

Rabbit Polyclonal PSMD5 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PSMD5 aa 300-400.

View Alternative Names

KIAA0072, PSMD5, 26S proteasome non-ATPase regulatory subunit 5, 26S protease subunit S5 basic, 26S proteasome subunit S5B

2 Images

Supplier Data

Immunoprecipitation - Anti-PSMD5 antibody (AB226371)

PSMD5 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226371 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab226371 at 1 μg/ml.

Lane 1 : ab226371 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-PSMD5 antibody (ab226371)

Predicted band size: 56 kDa

false

Supplier Data

Western blot - Anti-PSMD5 antibody (AB226371)

Cell lysates were prepared with NETN lysis buffer.

All lanes:

Western blot - Anti-PSMD5 antibody (ab226371) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 56 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human PSMD5 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

Q16401

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Giant Panda": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab226371 was affinity purified using an epitope specific to PSMD5 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSMD5 also known as S13 or 26S proteasome non-ATPase regulatory subunit 5 plays an essential role in the ubiquitin-proteasome system. It has a molecular mass of approximately 39 kDa. PSMD5 is a component of the 26S proteasome complex a structure important for protein degradation in cells. This protein locates in various tissues especially in high-protein turnover areas such as the liver skeletal muscle and heart.

Biological function summary

The protein influences the breakdown of ubiquitinated proteins. PSMD5 forms part of the 19S regulatory subunit of the 26S proteasome complex. The 19S regulatory complex is significant for recognizing and unfolding ubiquitinated substrates guiding their entry into the proteolytic 20S core. By participating in this process PSMD5 aids in maintaining protein quality control and regulating the cell cycle.

Pathways

PSMD5 affects the ubiquitin-proteasome degradation pathway an important regulator of protein turnover. This pathway is essential for numerous cellular processes including cell cycle progression and signal transduction. PSMD5 interacts with proteins such as ubiquitin and other subunits of the proteasome complex to facilitate the degradation of proteins tagged for destruction. It also plays a part in pathways linked to NF-kB signaling where it may interface with proteins like IκB a known inhibitor of the NF-kB pathway.

PSMD5 connects to conditions such as cancer and neurodegenerative diseases. The protein's role in the regulation of protein degradation implicates it in diseases where protein homeostasis goes awry. For example alterations in proteasome activity can lead to the accumulation of damaged proteins a hallmark of neurodegenerative disorders. In cancer dysregulation of protein degradation may contribute to uncontrolled cell growth and survival. Elevated levels of ubiquitin-like proteins often accompany such pathological conditions highlighting PSMD5's involvement.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD5 : PSMC2 : PSMC1 : PSMD2 module which probably assembles with a PSMD10 : PSMC4 : PSMC5 : PAAF1 module followed by dissociation of PSMD5.

See full target information PSMD5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:1172 PubMed33608523

2021

20S proteasomes secreted by the malaria parasite promote its growth.

Applications

Unspecified application

Species

Unspecified reactive species

Elya Dekel,Dana Yaffe,Irit Rosenhek-Goldian,Gili Ben-Nissan,Yifat Ofir-Birin,Mattia I Morandi,Tamar Ziv,Xavier Sisquella,Matthew A Pimentel,Thomas Nebl,Eugene Kapp,Yael Ohana Daniel,Paula Abou Karam,Daniel Alfandari,Ron Rotkopf,Shimrit Malihi,Tal Block Temin,Debakshi Mullick,Or-Yam Revach,Ariel Rudik,Nir S Gov,Ido Azuri,Ziv Porat,Giulia Bergamaschi,Raya Sorkin,Gijs J L Wuite,Ori Avinoam,Teresa G Carvalho,Sidney R Cohen,Michal Sharon,Neta Regev-Rudzki

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PSMD5 antibody

Immunoprecipitation - Anti-PSMD5 antibody (AB226371)

Western blot - Anti-PSMD5 antibody (AB226371)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise