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AB247004

Anti-PSMD6 antibody

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(1 Publication)

Rabbit Polyclonal PSMD6 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PSMD6 aa 1-150.

View Alternative Names

KIAA0107, PFAAP4, PSMD6, 26S proteasome non-ATPase regulatory subunit 6, 26S proteasome regulatory subunit RPN7, 26S proteasome regulatory subunit S10, Breast cancer-associated protein SGA-113M, Phosphonoformate immuno-associated protein 4, Proteasome regulatory particle subunit p44S10, p42A

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-PSMD6 antibody (AB247004)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PSMD6 antibody (AB247004)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for PSMD6 (green) using ab247004 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD6 antibody (AB247004)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD6 antibody (AB247004)

Paraffin-embedded human cerebral cortex tissue stained for PSMD6 using ab247004 at 1/2500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD6 antibody (AB247004)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD6 antibody (AB247004)

Paraffin-embedded human testis tissue stained for PSMD6 using ab247004 at 1/2500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD6 antibody (AB247004)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD6 antibody (AB247004)

Paraffin-embedded human placenta tissue stained for PSMD6 using ab247004 at 1/2500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD6 antibody (AB247004)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD6 antibody (AB247004)

Paraffin-embedded human skin tissue stained for PSMD6 using ab247004 at 1/2500 dilution in immunohistochemical analysis.

Western blot - Anti-PSMD6 antibody (AB247004)
  • WB

Unknown

Western blot - Anti-PSMD6 antibody (AB247004)

All lanes:

Western blot - Anti-PSMD6 antibody (ab247004) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) whole cell lysate

Predicted band size: 46 kDa

false

Western blot - Anti-PSMD6 antibody (AB247004)
  • WB

Unknown

Western blot - Anti-PSMD6 antibody (AB247004)

All lanes:

Western blot - Anti-PSMD6 antibody (ab247004) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lane 2:

NBT-II (rat Wistar bladder tumor cell line) whole cell lysate

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PSMD6 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q15008

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Primary Antibodies

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2500 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSMD6 also known as Proteasome 26S Subunit Non-ATPase 6 is an essential protein component of the 26S proteasome. Its molecular weight is approximately 37 kDa. The proteasome system where PSMD6 plays a critical role is responsible for the regulated degradation of ubiquitinated proteins which affects various cellular processes. This subunit predominantly locates in the cytoplasm and the nucleus where high proteasome activity is required such as in the heart liver and skeletal muscle cells.
Biological function summary

PSMD6 functions in the context of the proteasome complex's regulatory particle as it assists in maintaining protein homeostasis by recognizing and processing ubiquitin-tagged proteins. This process is fundamental for the proteins' degradation influencing cell cycle progression apoptosis and DNA repair. It ensures cellular quality control and adaptation to changing conditions. PSMD6 by participating in this mechanism indirectly supports the regulation of numerous cellular activities important for maintaining specific cellular functions.

Pathways

PSMD6 integrates into ubiquitin-proteasome pathways important for protein turnover and signaling transduction. It interacts with other proteasome subunits and related proteins like PSMD1 and PSMD2 in these pathways. The ubiquitin-proteasome system is pivotal for controlling protein levels involved in cell cycle regulation and signal transduction pathways such as the NF-kB signaling pathway. Alterations in these pathways might lead to disrupted cellular regulation causing various pathophysiological outcomes.

PSMD6 links to certain pathological conditions including cancer and neurodegenerative disorders. Abnormal proteasome activity where PSMD6 contributes often correlates with tumorigenesis as improper protein degradation can lead to uncontrolled cell proliferation. Furthermore changes in PSMD6 expression or function can associate with Alzheimer's disease where proteasome dysfunction results in the accumulation of misfolded or damaged proteins contributing to neurodegeneration. PSMD6 interacts with proteins such as ubiquitin and other proteasome subunits influencing these disease processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.
See full target information PSMD6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

FEBS open bio 12:1857-1874 PubMed36052535

2022

A cohesin-associated gene score may predict immune checkpoint blockade in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Cui-Zhen Liu,Jian-Di Li,Gang Chen,Rong-Quan He,Rui Lin,Zhi-Guang Huang,Jian-Jun Li,Xiu-Fang Du,Xiao-Ping Lv
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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