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AB140428

Anti-PSMD7/Mov34 antibody

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(4 Publications)

Rabbit Polyclonal PSMD7/Mov34 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PSMD7 aa 150-200.

View Alternative Names

MOV34L, PSMD7, 26S proteasome non-ATPase regulatory subunit 7, 26S proteasome regulatory subunit RPN8, 26S proteasome regulatory subunit S12, Mov34 protein homolog, Proteasome subunit p40

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD7/Mov34 antibody (AB140428)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD7/Mov34 antibody (AB140428)

Immunohistochemistry of human ovarian carcinoma using ab140428 at 1/1000. Detected using DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD7/Mov34 antibody (AB140428)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD7/Mov34 antibody (AB140428)

Immunohistochemistry of human lung carcinoma using ab140428 at 1/1000. Counterstained with hematoxylin, detected using DAB.

Immunoprecipitation - Anti-PSMD7/Mov34 antibody (AB140428)
  • IP

Unknown

Immunoprecipitation - Anti-PSMD7/Mov34 antibody (AB140428)

Detection of PSMD7/Mov34 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab140428 at 6 μg/mg lysate for IP and 1μg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-PSMD7/Mov34 antibody (ab140428)

Predicted band size: 37 kDa

false

Western blot - Anti-PSMD7/Mov34 antibody (AB140428)
  • WB

Unknown

Western blot - Anti-PSMD7/Mov34 antibody (AB140428)

All lanes:

Western blot - Anti-PSMD7/Mov34 antibody (ab140428) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 37 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human PSMD7 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

P51665

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Drosophila melanogaster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab140428 was affinity purified using an epitope specific to PSMD7/Mov34 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PSMD7 protein also known as Mov34 is a subunit of the 26S proteasome and plays an important role in protein degradation. The proteasome itself is a massive complex and PSMD7 is a 37 kDa protein component. Its primary site of expression is within the cytoplasm and nucleus of cells where it facilitates the breakdown of ubiquitinated proteins. This activity is essential for maintaining cellular protein homeostasis.
Biological function summary

The proper function of PSMD7 supports cell cycle regulation apoptosis and DNA damage repair. It exists as part of the 26S proteasome complex which is responsible for the regulated degradation of ubiquitin-conjugated proteins. This process controls various cellular mechanisms by ensuring that proteins are broken down after they have executed their function or if they become misfolded or damaged.

Pathways

The involvement of PSMD7 in proteolysis is central for processes such as the ubiquitin-proteasome pathway. This pathway is critical for protein turnover and the regulation of cellular processes including the cell cycle. PSMD7 works in tandem with other proteasomal subunits like PSMD4 and PSMD14 which help recruit and process ubiquitinated substrates.

The malfunction of PSMD7 associates with certain cancers and neurodegenerative diseases. Aberrations in proteasome function involving PSMD7 may lead to the accumulation of damaged proteins which contributes to cancer development through unchecked cell proliferation. In neurodegenerative disorders like Alzheimer's disease proteins like tau and beta-amyloid which should normally be degraded accumulate due to proteasomal dysfunction involving PSMD7.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.
See full target information PSMD7
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 14:35 PubMed38494478

2024

Deubiquitinase PSMD7 facilitates pancreatic cancer progression through activating Nocth1 pathway via modifying SOX2 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Luo,Yi Yu,Jinfeng Zhu,Leifeng Chen,Dan Li,Xingyu Peng,Zitao Liu,Qing Li,Qing Cao,Kai Huang,Rongfa Yuan

Molecular cell 84:522-537.e8 PubMed38151017

2023

hRpn13 shapes the proteome and transcriptome through epigenetic factors HDAC8, PADI4, and transcription factor NF-κB p50.

Applications

Unspecified application

Species

Unspecified reactive species

Vasty Osei-Amponsa,Monika Chandravanshi,Xiuxiu Lu,Valentin Magidson,Sudipto Das,Thorkell Andresson,Marzena Dyba,Venkata R Sabbasani,Rolf E Swenson,Caroline Fromont,Biraj Shrestha,Yongmei Zhao,Michelle E Clapp,Raj Chari,Kylie J Walters

Pharmacology 108:47-60 PubMed36423586

2022

Luteolin Attenuates Diabetic Myocardial Hypertrophy by Inhibiting Proteasome Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Bing Li,Mubarak Rekep,Jia-Hui Tian,Qian Wu,Mei Chen,Shuo Yang,Lu-Xuan Zhang,Gui-Ping Zhang,Yuan Qin,Xi-Yong Yu,Qin Xue,Ying-Hua Liu

Nature communications 11:1291 PubMed32157086

2020

Structure of E3 ligase E6AP with a proteasome-binding site provided by substrate receptor hRpn10.

Applications

Unspecified application

Species

Unspecified reactive species

Gwen R Buel,Xiang Chen,Raj Chari,Maura J O'Neill,Danielle L Ebelle,Conor Jenkins,Vinidhra Sridharan,Sergey G Tarasov,Nadya I Tarasova,Thorkell Andresson,Kylie J Walters
View all publications
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