AB246883

Anti-PSMD8 antibody

Name: Anti-PSMD8 antibody (ab246883) | Abcam
Brand: Abcam Limited
SKU: AB246883
Price: 655 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal PSMD8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human 26S proteasome non-ATPase regulatory subunit 8 aa 200 to C-terminus.

View Alternative Names

26S proteasome non-ATPase regulatory subunit 8, 26S proteasome regulatory subunit RPN12, 26S proteasome regulatory subunit S14, p31, PSMD8

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD8 antibody (AB246883)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD8 antibody (AB246883)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum tissue labelling PSMD8 with ab246883 at 1/20 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD8 antibody (AB246883)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling PSMD8 with ab246883 at 1/20 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD8 antibody (AB246883)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling PSMD8 with ab246883 at 1/20 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD8 antibody (AB246883)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling PSMD8 with ab246883 at 1/20 dilution.

Unknown

Western blot - Anti-PSMD8 antibody (AB246883)

All lanes:

Western blot - Anti-PSMD8 antibody (ab246883) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Predicted band size: 40 kDa

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Unknown

Western blot - Anti-PSMD8 antibody (AB246883)

All lanes:

Western blot - Anti-PSMD8 antibody (ab246883) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 40 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human 26S proteasome non-ATPase regulatory subunit 8 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P48556

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSMD8 also known as proteasome 26S subunit non-ATPase 8 is a component of the 19S regulatory particle of the proteasome complex. This protein has a molecular mass of approximately 37 kDa. It facilitates protein degradation by recognizing ubiquitin-tagged substrates important for maintaining protein homeostasis. Researchers have identified its expression in various tissues including liver kidney and heart and it plays a significant role in cellular processes that require regulated protein turnover.

Biological function summary

PSMD8 contributes to the assembly and function of the 26S proteasome which is a multi-subunit complex responsible for degrading ubiquitinated proteins. It acts as a part of the regulatory particle enabling substrate recognition and unfolding. Through its function PSMD8 is involved in the ubiquitin-proteasome system which is key in cellular regulation mechanisms such as cell cycle control apoptosis and DNA repair. This highlights its importance in maintaining cell function and integrity.

Pathways

Protein homeostasis and degradation represent important pathways in which PSMD8 participates. It plays a role in the ubiquitin-proteasome pathway interacting with proteins within this system like Rpn11 and Rpt5 which are also parts of the proteasome complex. Additionally the involvement of PSMD8 in the protein degradation pathway links it to the regulation of transcription factors influencing various signaling pathways that control cell proliferation and differentiation.

PSMD8 has implications in neurodegenerative diseases and cancer as disruptions in proteasome functions are often related to these conditions. Altered proteasome activity can lead to the accumulation of misfolded or damaged proteins which is a hallmark of neurodegenerative diseases such as Parkinson's disease. Moreover in cancer PSMD8's role in protein degradation can affect cell cycle regulation working in connection with proteins like p53. These relationships make PSMD8 an interesting target for therapeutic interventions in proteasome-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.

See full target information 26S proteasome non-ATPase regulatory subunit 8

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 23:586 PubMed37365518

2023

Expression profiles and functional prediction of histone acetyltransferases of the MYST family in kidney renal clear cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Liang,Xiangke Li,Xiaoman Shen,Runlei Yang,Chuan Chen

BMC cancer 23:573 PubMed37349676

2023

PSMD8 can serve as potential biomarker and therapeutic target of the PSMD family in ovarian cancer: based on bioinformatics analysis and in vitro validation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Li,Xinru Li,Yuexin Hu,Ouxuan Liu,Yuxuan Wang,Siting Li,Qing Yang,Bei Lin

Autophagy 18:1763-1784 PubMed34836490

2021

HYPK coordinates degradation of polyneddylated proteins by autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Debasish Kumar Ghosh,Akash Ranjan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PSMD8 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD8 antibody (AB246883)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD8 antibody (AB246883)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD8 antibody (AB246883)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD8 antibody (AB246883)

Western blot - Anti-PSMD8 antibody (AB246883)

Western blot - Anti-PSMD8 antibody (AB246883)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise