Rabbit Polyclonal PSMD9 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human 26S proteasome non-ATPase regulatory subunit 9.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human 26S proteasome non-ATPase regulatory subunit 9. Database link O00233
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.5-5 µg/mL | Notes - |
Species Human | Dilution info 0.5-5 µg/mL | Notes - |
Species Recombinant fragment - Human | Dilution info 0.5-5 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5-20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5-20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process.
Alternative names
26S proteasome non-ATPase regulatory subunit 9, 26S proteasome regulatory subunit p27, PSMD9
Recommended products
Rabbit Polyclonal PSMD9 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human 26S proteasome non-ATPase regulatory subunit 9.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human 26S proteasome non-ATPase regulatory subunit 9. Database link O00233
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PSMD9 also known as 26S proteasome non-ATPase regulatory subunit 9 or Bridge-1 is a component of the 26S proteasome complex. This protein has a mass of approximately 49 kDa. It plays a role in regulating the degradation of ubiquitinated proteins through its function in the proteasome complex. The protein is expressed in multiple tissues including skeletal muscle and heart.
- Biological function summary
PSMD9 participates in proteasomal degradation of intracellular proteins facilitating protein turnover and maintaining cellular homeostasis. It integrates into the 26S proteasome complex consisting of the 20S core particle and the 19S regulatory particle. Within the proteasome PSMD9 interacts with various subunits to regulate the recognition and unfolding of ubiquitinated substrates.
- Pathways
PSMD9 plays an important role in the ubiquitin-proteasome pathway which is essential for protein regulation and quality control in cells. This pathway involves tagging proteins with ubiquitin for degradation. PSMD9 shows functional relationships with other proteasome subunits like PSMD4 and PSMD11 which participate in similar regulation processes.
- Associated diseases and disorders
Dysfunction of the ubiquitin-proteasome pathway involving PSMD9 contributes to neurodegenerative diseases such as Alzheimer's disease and certain types of cancer. In Alzheimer's improper proteasomal activity can lead to the accumulation of damaged proteins. PSMD9's activity links it to other proteins implicated in these diseases including PSMD1 and the amyloid precursor protein in Alzheimer's disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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9 product images
Western blot - Anti-PSMD9 antibody (ab233154)
All lanes: Western blot - Anti-PSMD9 antibody (ab233154) at 3 µg/mL
Lane 1: Human liver lysate
Lane 2: Mouse spleen lysate
Lane 3: Mouse heart lysate
Lane 4: A549 (Human lung carcinoma cell line) cell lysate
Lane 5: Mouse placenta lysate
SecondaryAll lanes: HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PSMD9 antibody (ab233154)
A549 (Human lung carcinoma cell line) cells stained for PSMD9 using ab233154 (green) at 20 μg/mL in ICC/IF analysis. FITC-labeled secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD9 antibody (ab233154)
Formalin-fixed, paraffin-embedded human prostate tissue stained for PSMD9 using ab233154 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD9 antibody (ab233154)
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for PSMD9 using ab233154 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD9 antibody (ab233154)
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for PSMD9 using ab233154 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD9 antibody (ab233154)
Formalin-fixed, paraffin-embedded human glioma tissue stained for PSMD9 using ab233154 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD9 antibody (ab233154)
Formalin-fixed, paraffin-embedded human liver tissue stained for PSMD9 using ab233154 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD9 antibody (ab233154)
Formalin-fixed, paraffin-embedded human liver cancer tissue stained for PSMD9 using ab233154 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Western blot - Anti-PSMD9 antibody (ab233154)
All lanes: Western blot - Anti-PSMD9 antibody (ab233154) at 2 µg/mL
All lanes: Recombinant human PSMD9 protein
Predicted band size: 13 kDa, 25 kDa
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