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AB155091

Anti-PSME1 antibody [EPR10967(B)]

5

(4 Reviews)

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(7 Publications)

Rabbit Recombinant Monoclonal PSME1 antibody. Suitable for WB, I-ELISA, ICC/IF and reacts with Human samples. Cited in 7 publications.

View Alternative Names

IFI5111, PSME1, Proteasome activator complex subunit 1, 11S regulator complex subunit alpha, Activator of multicatalytic protease subunit 1, Interferon gamma up-regulated I-5111 protein, Proteasome activator 28 subunit alpha, REG-alpha, IGUP I-5111, PA28a, PA28alpha

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (AB155091)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (AB155091)

Immunofluorescent analysis of MCF7 cells labeling PSME1 with ab155091 at 1/100 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (AB155091)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (AB155091)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling PSME1 with purified ab155091 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

Indirect ELISA - Anti-PSME1 antibody [EPR10967(B)] (AB155091)
  • I-ELISA

Lab

Indirect ELISA - Anti-PSME1 antibody [EPR10967(B)] (AB155091)

Indirect ELISA analysis of Human Proteasome activator complex subunit 1 using ab155091 at 1000 ng/mL dilution and secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 2500 ng/mL dilution

Western blot - Anti-PSME1 antibody [EPR10967(B)] (AB155091)
  • WB

Lab

Western blot - Anti-PSME1 antibody [EPR10967(B)] (AB155091)

Lanes 1-4 : Merged signal (red and green). Green - ab155091 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.

ab155091 Anti-PSME1 antibody [EPR10967(B)] was shown to specifically react with PSME1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266192 (knockout cell lysate ab257613) was used. Wild-type and PSME1 knockout samples were subjected to SDS-PAGE. ab155091 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

PSME1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

Western blot - Human PSME1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-psme1-knockout-hek-293t-cell-line-ab266192'>ab266192</a>)

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 4:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Western blot - Anti-PSME1 antibody [EPR10967(B)] (AB155091)
  • WB

Unknown

Western blot - Anti-PSME1 antibody [EPR10967(B)] (AB155091)

All lanes:

Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 29 kDa

false

Western blot - Anti-PSME1 antibody [EPR10967(B)] (AB155091)
  • WB

AbReview40987****

Western blot - Anti-PSME1 antibody [EPR10967(B)] (AB155091)

All lanes:

Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution

All lanes:

Human prostate cancer whole tissue lysate at 20 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/5000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

true

Exposure time: 2min

This image is courtesy of an anonymous Abreview

  • Carrier free

    Anti-PSME1 antibody [EPR10967(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10967(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, I-ELISA, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p>Use at 31.25 ng /ml</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/100 - 1/250. </strong></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSME1 also known as PA28α (proteasome activator complex subunit 1) is a protein that functions as a regulatory component of the 20S proteasome. It has a molecular weight of approximately 28 kDa. This protein is expressed in various tissues with noticeable expression in immune-related tissues such as the spleen and thymus. PSME1 binds to proteasome core particles and modifies their activity by opening access to their catalytic core honing the degradation of ubiquitinated proteins.
Biological function summary

PSME1 modulates protein degradation processes and participates as a component of the immunoproteasome. The immunoproteasome is a complex that processes peptides for presentation on MHC class I molecules vital for immune response. This involvement transforms PSME1 into an important player in antigen processing and presentation influencing T-cell activation and immune surveillance. PSME1 affects immune system adaptability and efficiency impacting infection response and immune regulation.

Pathways

PSME1 is a significant participant in the ubiquitin-proteasome system and the MHC class I antigen processing pathway. It directly interacts with other proteasome components like the catalytic 20S core and the 11S regulator. The interplay with these proteins steers the precision and output of antigenic peptides essential for T-cell mediated immune recognition. By influencing these pathways PSME1 links the protein degradation machinery to adaptive immunity.

PSME1 has connections to autoimmune diseases and cancers. Alterations in its function or expression can disrupt regular proteasome activity leading to improper immune responses such as in autoimmune pathologies like systemic lupus erythematosus. Furthermore PSME1's role in antigen processing intersects with other proteins such as TAP1 affecting immune evasion strategies seen in certain cancers. These interactions suggest that PSME1 is a contributor in both immune-related health issues and cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.
See full target information PSME1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer therapeutics 23:1743-1760 PubMed39210605

2024

Immunoproteasome Activation Expands the MHC Class I Immunopeptidome, Unmasks Neoantigens, and Enhances T-cell Anti-Myeloma Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Priyanka S Rana,James J Ignatz-Hoover,Chunna Guo,Amber L Mosley,Ehsan Malek,Yuriy Federov,Drew J Adams,James J Driscoll

Cell 186:5411-5427.e23 PubMed37918396

2023

The proteomic landscape of synaptic diversity across brain regions and cell types.

Applications

Unspecified application

Species

Unspecified reactive species

Marc van Oostrum,Thomas M Blok,Stefano L Giandomenico,Susanne Tom Dieck,Georgi Tushev,Nicole Fürst,Julian D Langer,Erin M Schuman

STAR protocols 2:100526 PubMed34027484

2021

In-gel proteasome assay to determine the activity, amount, and composition of proteasome complexes from mammalian cells or tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Ayse Seda Yazgili,Thomas Meul,Vanessa Welk,Nora Semren,Ilona E Kammerl,Silke Meiners

Journal of lipid and atherosclerosis 10:223-239 PubMed34095014

2021

Phosphorylation in Novel Mitochondrial Creatine Kinase Tyrosine Residues Render Cardioprotection against Hypoxia/Reoxygenation Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Nammi Park,Jubert Marquez,Maria Victoria Faith Garcia,Ippei Shimizu,Sung Ryul Lee,Hyoung Kyu Kim,Jin Han

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 20:399-410 PubMed31595669

2019

Proteasomal adaptations underlying carfilzomib-resistance in human bone marrow plasma cells.

Applications

Unspecified application

Species

Unspecified reactive species

E Steve Woodle,Simon Tremblay,Paul Brailey,Alin Girnita,Rita R Alloway,Bruce Aronow,Nupur Dasgupta,Frederic Ebstein,Peter-Michael Kloetzel,Min Jae Lee,Kyung B Kim,Harinder Singh,James J Driscoll

Nature microbiology 4:933-940 PubMed30886358

2019

Immunoproteasome activation enables human TRIM5α restriction of HIV-1.

Applications

Unspecified application

Species

Unspecified reactive species

Jose M Jimenez-Guardeño,Luis Apolonia,Gilberto Betancor,Michael H Malim

The Journal of biological chemistry 291:13147-59 PubMed27129254

2016

Inhibition of Proteasome Activity Induces Formation of Alternative Proteasome Complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa Welk,Olivier Coux,Vera Kleene,Claire Abeza,Dietrich Trümbach,Oliver Eickelberg,Silke Meiners
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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