Anti-PSME1 antibody [EPR10967(B)] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] - BSA and Azide free (AB249185)

This data was developed using ab155091, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of MCF7 cells labeling PSME1 with ab155091 at 1/100 dilution.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] - BSA and Azide free (AB249185)

This data was developed using ab155091, the same antibody clone in a different buffer formulation.

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling PSME1 with purified ab155091 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor^®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

• WB

Lab

Western blot - Anti-PSME1 antibody [EPR10967(B)] - BSA and Azide free (AB249185)

This data was developed using ab155091, the same antibody clone in a different buffer formulation.

Lanes 1-4 : Merged signal (red and green). Green - ab155091 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.

ab155091 Anti-PSME1 antibody [EPR10967(B)] was shown to specifically react with PSME1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266192 (knockout cell lysate ab257613) was used. Wild-type and PSME1 knockout samples were subjected to SDS-PAGE. ab155091 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PSME1 antibody [EPR10967(B)] (<a href='/en-us/products/primary-antibodies/psme1-antibody-epr10967b-ab155091'>ab155091</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

PSME1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

Western blot - Human PSME1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-psme1-knockout-hek-293t-cell-line-ab266192'>ab266192</a>)

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 4:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

• WB

Unknown

Western blot - Anti-PSME1 antibody [EPR10967(B)] - BSA and Azide free (AB249185)

This data was developed using ab155091, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PSME1 antibody [EPR10967(B)] (<a href='/en-us/products/primary-antibodies/psme1-antibody-epr10967b-ab155091'>ab155091</a>) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 29 kDa

false