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AB155985

Anti-PSME1 antibody [EPR10968(B)]

5

(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal PSME1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

IFI5111, PSME1, Proteasome activator complex subunit 1, 11S regulator complex subunit alpha, Activator of multicatalytic protease subunit 1, Interferon gamma up-regulated I-5111 protein, Proteasome activator 28 subunit alpha, REG-alpha, IGUP I-5111, PA28a, PA28alpha

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME1 antibody [EPR10968(B)] (AB155985)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME1 antibody [EPR10968(B)] (AB155985)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PSME1 with ab155985 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME1 antibody [EPR10968(B)] (AB155985)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME1 antibody [EPR10968(B)] (AB155985)

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling PSME1 with ab155985 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-PSME1 antibody [EPR10968(B)] (AB155985)
  • WB

Unknown

Western blot - Anti-PSME1 antibody [EPR10968(B)] (AB155985)

All lanes:

Western blot - Anti-PSME1 antibody [EPR10968(B)] (ab155985) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

MCF-7 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 29 kDa

false

Western blot - Anti-PSME1 antibody [EPR10968(B)] (AB155985)
  • WB

Lab

Western blot - Anti-PSME1 antibody [EPR10968(B)] (AB155985)

Blocking and diluting buffer : Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS

Lanes 1-4 : Merged signal (red and green). Green - ab155985 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.

All lanes:

Western blot - Anti-PSME1 antibody [EPR10968(B)] (ab155985) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

PSME1 knockout HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/10000 dilution

Predicted band size: 29 kDa

false

  • Carrier free

    Anti-PSME1 antibody [EPR10968(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10968(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSME1 also known as PA28α (proteasome activator complex subunit 1) is a protein that functions as a regulatory component of the 20S proteasome. It has a molecular weight of approximately 28 kDa. This protein is expressed in various tissues with noticeable expression in immune-related tissues such as the spleen and thymus. PSME1 binds to proteasome core particles and modifies their activity by opening access to their catalytic core honing the degradation of ubiquitinated proteins.
Biological function summary

PSME1 modulates protein degradation processes and participates as a component of the immunoproteasome. The immunoproteasome is a complex that processes peptides for presentation on MHC class I molecules vital for immune response. This involvement transforms PSME1 into an important player in antigen processing and presentation influencing T-cell activation and immune surveillance. PSME1 affects immune system adaptability and efficiency impacting infection response and immune regulation.

Pathways

PSME1 is a significant participant in the ubiquitin-proteasome system and the MHC class I antigen processing pathway. It directly interacts with other proteasome components like the catalytic 20S core and the 11S regulator. The interplay with these proteins steers the precision and output of antigenic peptides essential for T-cell mediated immune recognition. By influencing these pathways PSME1 links the protein degradation machinery to adaptive immunity.

PSME1 has connections to autoimmune diseases and cancers. Alterations in its function or expression can disrupt regular proteasome activity leading to improper immune responses such as in autoimmune pathologies like systemic lupus erythematosus. Furthermore PSME1's role in antigen processing intersects with other proteins such as TAP1 affecting immune evasion strategies seen in certain cancers. These interactions suggest that PSME1 is a contributor in both immune-related health issues and cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.
See full target information PSME1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular biosciences 8:806650 PubMed35096971

2022

Accumulated ROS Activates HIF-1α-Induced Glycolysis and Exerts a Protective Effect on Sensory Hair Cells Against Noise-Induced Damage.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Liang,Shuohui Dong,Wenwen Liu,Man Wang,Shanshan Tian,Yu Ai,Haibo Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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