Anti-PSME2 antibody [EPR14931] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (AB250700)

This data was developed using ab183727, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling PSME2 with ab183727 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (AB250700)

This data was developed using ab183727, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling PSME2 with ab183727 at 1/210 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (AB250700)

This data was developed using ab183727, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labelling PSME2 with ab183727 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (AB250700)

This data was developed using ab183727, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling PSME2 with ab183727 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (AB250700)

This data was developed using ab183727, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling PSME2 with ab183727 at 1/500 dilution, followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (AB250700)

This data was developed using ab183727, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling PSME2 with ab183727 at 1/500 dilution, followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

• I-ELISA

Lab

Indirect ELISA - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (AB250700)

This data was developed using ab183727, the same antibody clone in a different buffer formulation.ELISA analysis of Human PSME2 recombinant protein at 250 ng/mL with ab183727. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (AB250700)

This data was developed using ab183727, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling PSME2 with ab183727 at 1/500 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• WB

Supplier Data

Western blot - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (AB250700)

This data was developed using ab183727, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PSME2 antibody [EPR14931] (<a href='/en-us/products/primary-antibodies/psme2-antibody-epr14931-ab183727'>ab183727</a>) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

PC3 cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 27 kDa

false

• WB

Supplier Data

Western blot - Anti-PSME2 antibody [EPR14931] - BSA and Azide free (AB250700)

This data was developed using ab183727, the same antibody clone in a different buffer formulation.

Exposure times :

Lane 1 : 3.25 seconds

Lane 2 : 3 minutes

Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-PSME2 antibody [EPR14931] (<a href='/en-us/products/primary-antibodies/psme2-antibody-epr14931-ab183727'>ab183727</a>) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 2:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 3:

4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

false