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AB157157

Anti-PSME3 antibody

5

(2 Reviews)

|

(11 Publications)

Rabbit Polyclonal PSME3 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human PSME3 aa 200 to C-terminus.

View Alternative Names

Proteasome activator complex subunit 3, 11S regulator complex subunit gamma, Activator of multicatalytic protease subunit 3, Ki nuclear autoantigen, Proteasome activator 28 subunit gamma, REG-gamma, PA28g, PA28gamma, PSME3

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME3 antibody (AB157157)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME3 antibody (AB157157)

Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections using ab117809 at 1/1000 dilution.

Immunoprecipitation - Anti-PSME3 antibody (AB157157)
  • IP

Unknown

Immunoprecipitation - Anti-PSME3 antibody (AB157157)

Detection of PMSE3 by Western Blot of Immunprecipitate.
ab157157 at 1µg/ml labeling PMSE3 in 293T whole cell lysate immunoprecipitated using ab157157 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-PSME3 antibody (ab157157)

Predicted band size: 29 kDa

false

Immunoprecipitation - Anti-PSME3 antibody (AB157157)
  • IP

AbReview42190****

Immunoprecipitation - Anti-PSME3 antibody (AB157157)

ab157157 Immunoprecipitating PSME3 in human HEK293 whole cell lysate. 250μg of cell lysate was incubated with the undiluted primary antibody and matrix (Protein G) for 18 hours at 4°C. For western blotting ab157157 (1/1000) was used to confirm successful immunoprecipation.

All lanes:

Immunoprecipitation - Anti-PSME3 antibody (ab157157)

Predicted band size: 29 kDa

false

This image is courtesy of an anonymous Abreview

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME3 antibody (AB157157)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSME3 antibody (AB157157)

Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal carcinoma tissue sections using ab117809 at 1/1000 dilution.

Western blot - Anti-PSME3 antibody (AB157157)
  • WB

Unknown

Western blot - Anti-PSME3 antibody (AB157157)

All lanes:

Western blot - Anti-PSME3 antibody (ab157157) at 1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 29 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human PSME3 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P61289

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Reactivity data

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"IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebra finch": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab157157 was affinity purified using an epitope specific to PSME3 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PSME3 protein also known as PA28γ or REGγ is mechanically an essential proteasome activator that facilitates the degradation of ubiquitin-independent substrates. It has a molecular weight of approximately 29 kDa. PSME3 is mainly found in the nucleus and shows strong expression in various tissues including liver kidney and heart. The protein operates by binding to the 20S proteasome core and enhances the specificity of proteolytic activity.
Biological function summary

PSME3 regulates cell cycle progression and apoptosis. It is part of a larger multiprotein complex with the 20S proteasome core forming an important component in protein homeostasis. PSME3’s interaction with the proteasome core significantly reduces the accumulation of certain regulatory proteins by promoting their degradation. This function affects several cellular processes such as cell proliferation and DNA repair aiding the maintenance of cellular function and integrity.

Pathways

PSME3 plays a critical role in mediating the ubiquitin-proteasome pathway and the MDM2-p53 pathway. In the ubiquitin-proteasome pathway it assists in the controlled turnover of intracellular proteins which is pivotal for maintaining cellular protein quality. In the MDM2-p53 pathway it affects the stability of the p53 protein by enhancing the degradation of both MDM2 and p53 therefore modulating apoptosis and growth arrest in cells under stress or DNA damage conditions.

Disruptions in PSME3 function correlate with cancer and neurodegenerative disorders. Overexpression of PSME3 is often linked to oncogenesis due to its role in controlling the degradation of tumor suppressor proteins like p53. This protein is also implicated in Parkinson’s disease where its interaction with pathogenic proteins suggests it might contribute to the accumulation of misfolded proteins. PSME3 along with its pathways and related proteins offers potential as a target for therapeutic intervention in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition (PubMed : 25361978).
See full target information PSME3
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed40091835

2025

Targeting ubiquitin-independent proteasome with small molecule increases susceptibility in pan-KRAS-mutant cancer models.

Applications

Unspecified application

Species

Unspecified reactive species

Shihui Shen,Qiansen Zhang,Yuhan Wang,Hui Chen,Shuangming Gong,Yun Liu,Conghao Gai,Hansen Chen,Enhao Zhu,Bo Yang,Lin Liu,Siyuan Cao,Mengting Zhao,Wenjie Ren,Mengjuan Li,Zhuoya Peng,Lu Zhang,Shaoying Zhang,Juwen Shen,Bianhong Zhang,Patrick Kh Lee,Kun Li,Lei Li,Huaiyu Yang

Cell death & disease 15:703 PubMed39349939

2024

Ubiquitin-independent degradation of Bim blocks macrophage pyroptosis in sepsis-related tissue injury.

Applications

Unspecified application

Species

Unspecified reactive species

Peilin Shi,Yingying Du,Yunyan Zhang,Bo Yang,Qiujing Guan,Yiming Jing,Hao Tang,Jianguo Tang,Chunhui Yang,Xiaoli Ge,Shihui Shen,Lei Li,Chunrong Wu

Molecular cancer therapeutics 23:1743-1760 PubMed39210605

2024

Immunoproteasome Activation Expands the MHC Class I Immunopeptidome, Unmasks Neoantigens, and Enhances T-cell Anti-Myeloma Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Priyanka S Rana,James J Ignatz-Hoover,Chunna Guo,Amber L Mosley,Ehsan Malek,Yuriy Federov,Drew J Adams,James J Driscoll

Molecular carcinogenesis 63:1874-1891 PubMed38888205

2024

ATP citrate lyase promotes the progression of hepatocellular carcinoma by activating the REGγ-proteasome pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qihong Cai,Honghua Zhu,Yile Dai,Qingqing Zhou,Qiyu Zhang,Qiandong Zhu

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed38471782

2024

PTPN11/Corkscrew Activates Local Presynaptic Mapk Signaling to Regulate Synapsin, Synaptic Vesicle Pools, and Neurotransmission Strength, with a Dual Requirement in Neurons and Glia.

Applications

Unspecified application

Species

Unspecified reactive species

Shannon N Leahy,Dominic J Vita,Kendal Broadie

PLoS biology 21:e3001969 PubMed36701299

2023

FMRP activity and control of Csw/SHP2 translation regulate MAPK-dependent synaptic transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Shannon N Leahy,Chunzhu Song,Dominic J Vita,Kendal Broadie

Food & function 13:10923-10936 PubMed36205648

2022

Isoquercitrin from L. produces an anti-obesity effect on obese mice by targeting C-1-tetrahydrofolate synthase, carbonyl reductase, and glutathione -transferase P and modification of the AMPK/SREBP-1c/FAS/CD36 signaling pathway in mice .

Applications

Unspecified application

Species

Unspecified reactive species

Majid Manzoor,Makoto Muroi,Naoko Ogawa,Hiroki Kobayashi,Haruna Nishimura,Danni Chen,Opeyemi B Fasina,Jianyu Wang,Hiroyuki Osada,Minoru Yoshida,Lan Xiang,Jianhua Qi

Cell death and differentiation 27:2952-2972 PubMed32424140

2020

The proteasome activator REGγ accelerates cardiac hypertrophy by declining PP2Acα-SOD2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Xie,Yang Gao,Rifeng Gao,Wenlong Yang,Zheng Dong,Robb E Moses,Aijun Sun,Xiaotao Li,Junbo Ge

European journal of pharmacology 865:172748 PubMed31655030

2019

Fisetin, via CKIP-1/REGγ, limits oxidized LDL-induced lipid accumulation and senescence in RAW264.7 macrophage-derived foam cells.

Applications

Unspecified application

Species

Unspecified reactive species

QingLing Jia,Hui Cao,DingZhu Shen,Li Yan,Chuan Chen,Sanli Xing

Molecular medicine reports 19:4561-4568 PubMed30942447

2019

MicroRNA‑195‑5p inhibitor prevents the development of osteoarthritis by targeting REGγ.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Shu,Junpeng Long,Weixiong Guo,Wei Ye
View all publications
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